Peptide substrate identification for yeast Hsp40 Ydj

内容节点: 概述; 实验/设备条件; 样品提取; 实验/操作方法; 实验结果/结论; 仪器/耗材清单

· This paper discusses the identification of a peptide substrate for molecular chaperone Hsp40 Ydj1 using combination of phage display library screening and ITC · Molecular chaperone heat shock protein 40s (Hsp40s) play roles with Hsp70s to promote protein folding, assembly, translocation, degradation, and prion propagation. Hsp40 proteins interact with hydrophobic side chains of non-native polypeptides to prevent the non-native polypeptides from aggregating. Mechanism for binding is not understood 差示扫描式微量量热仪MicroCal , USA

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