仪器网

欢迎您: 免费注册 仪器双拼网址:www.yiqi.com
首页-资讯-资料-产品-求购-招标-品牌-展会-行业应用-社区-供应商手机版
官方微信
中国仪器网• 行业应用
仪器网/ 行业应用/ 解决方案/ 吡咯里西啶生物碱的极ng确质量 Q-TOF LC/MS 及全离子 MS/M

吡咯里西啶生物碱的极ng确质量 Q-TOF LC/MS 及全离子 MS/M

点击这里给我发消息
内容节点
概述
实验/设备条件
样品提取
实验/操作方法
实验结果/结论
仪器/耗材清单
Pyrrolizidine
alkaloids (PAs) and their N
-
oxides (PANOs) have serious hepatotoxic
effects in both humans and animals, necessitating a method capable of detecting,
characterizing, and quantifying them using a robust assay. While triple quadrupole
LC/MS systems are ideal
for targeted detection, targeted analysis requires
standards that are not always easily obtained. Only a fraction of known naturally
occurring PAs are available as standards. Triple quadrupole analyses are also not
useful for screening unknowns. High
-
res
olution mass spectrometers that provide
accurate
-
mass data enable characterization of unknown PAs/PANOs without the
need for specific standards.
This application note presents a workflow that uses accurate
-
mass Q
-
TOF LC/MS,
with All Ions MS/MS and databas
e/mass spectral library searching, for the
selective and sensitive characterization, screening, tentative identification, and
quantification of PAs and PANOs in botanicals and dietary supplements.
Twenty
-
five PA/PANO standards were characterized using
their high
-
resolution
accurate
-
mass MS/MS spectra. These data were used to create a personal com
-
pound database and library (PCDL) that was then used to screen for PAs/PANOs in
44 botanical and dietary supplement samples. Quantitation was performed using
Q
-
TOF LC/MS response. PAs/PANOs not specifically targeted were tentatively
identified using characteristic fragment ions and fragmentation patterns. The
PAs/PANOs characterized could potentially lead to the discovery of more unique
PAs in additional botan
ical and dietary supplement sample
s. 6500 系列四极杆(Q-TOF) 液质联用系统
相关产品
相关解决方案
其他解决方案
最新解决方案
厂商产品资料
在线留言
官方微信

仪器网微信服务号

扫码获取最新信息


仪器网官方订阅号

扫码获取最新信息

在线客服

咨询客服

在线客服
工作日:  9:00-18:00
联系客服 企业专属客服
电话客服:  400-822-6768
工作日:  9:00-18:00
订阅商机

仪采招微信公众号

采购信息一键获取海量商机轻松掌控