Human CYP2C18 ELISA Kit

HumanCytochromeP4502C18(CYP2C18)ELISAKit

DL-CYP2C18-Hu

Overview：

Othernames：CYP2C;CPCI;CYP2C17;CPCJ;P450C2C;limonene6-monooxygenase;Mephenytoin4-hydroxylase;CytochromeP450Family2SubfamilyCPolypeptide18

Function:CytochromesP450areagroupofheme-thiolatemonooxygenases.Inlivermicrosomes,thisenzymeisinvolvedinanNADPH-dependentelectrontransportpathway.Itoxidizesavarietyofstructurallyunrelatedcompounds,includingsteroids,fattyacids,andxenobiotics.

Sequence：

MDPAVALVLCLSCLFLLSLWRQSSGRGRLPSGPTPLPIIGNILQLDVKDM

SKSLTNFSKVYGPVFTVYFGLKPIVVLHGYEAVKEALIDHGEEFSGRGSF

PVAEKVNKGLGILFSNGKRWKEIRRFCLMTLRNFGMGKRSIEDRVQEEAR

CLVEELRKTNASPCDPTFILGCAPCNVICSVIFHDRFDYKDQRFLNLMEK

FNENLRILSSPWIQVCNNFPALIDYLPGSHNKIAENFAYIKSYVLERIKE

HQESLDMNSARDFIDCFLIKMEQEKHNQQSEFTVESLIATVTDMFGAGTE

TTSTTLRYGLLLLLKYPEVTAKVQEEIECVVGRNRSPCMQDRSHMPYTDA

VVHEIQRYIDLLPTNLPHAVTCDVKFKNYLIPKGTTIITSLTSVLHNDKE

FPNPEMFDPGHFLDKSGNFKKSDYFMPFSAGKRMCMGEGLARMELFLFLT

TILQNFNLKSQVDPKDIDITPIANAFGRVPPLYQLCFIPV

2,Features

DL-CYP2C18-Hu

HumanCytochromeP4502C18(CYP2C18)ELISAKit

INTENDEDUSE

ThekitisasandwichenzymeimmunoassayforinvitroquantitativemeasurementofCYP2C18inhumantissuehomogenates,celllysatesandotherbiologicalfluids.

DETECTIONRANGE

0.625-40ng/mL.ThestandardcurveconcentrationsusedfortheELISA’swere40ng/mL,20ng/mL,10ng/mL,5ng/mL,2.5ng/mL,1.25ng/mL,0.625ng/mL.

SENSITIVITY

TheminimumdetectabledoseofCYP2C18istypicallylessthan0.247ng/mL.

Thesensitivityofthisassay,orLowerLimitofDetection(LLD)wasdefinedasthelowestproteinconcentrationthatcouldbedifferentiatedfromzero.Itwasdeterminedbyaddingtwostandarddeviationstothemeanopticaldensityvalueoftwentyzerostandardreplicatesandcalculatingthecorrespondingconcentration.

SPECIFICITY

ThisassayhashighsensitivityandexcellentspecificityfordetectionofCYP2C18.

Nosignificantcross-reactivityorinterferencebetweenCYP2C18andanalogueswasobserved.

Note:

Limitedbycurrentskillsandknowledge,itisimpossibleforustocompletethecross-reactivitydetectionbetweenCYP2C18andalltheanalogues,therefore,crossreactionmaystillexist.

PRECISION

Intra-assayPrecision(Precisionwithinanassay):3sampleswithlow,middleandhighlevelCYP2C18weretested20timesononeplate,respectively.

Inter-assayPrecision(Precisionbetweenassays):3sampleswithlow,middleandhighlevelCYP2C18weretestedon3differentplates,8replicatesineachplate.

CV(%)=SD/meanX100

Intra-Assay:CV<10%

Inter-Assay:CV<12%

STABILITY

ThestabilityofELISAkitisdeterminedbythelossrateofactivity.Thelossrateofthiskitislessthan5%withintheexpirationdateunderappropriatestoragecondition.

Note:

Tominimizeextrainfluenceontheperformance,operationproceduresandlabconditions,especiallyroomtemperature,airhumidity,incubatortemperature首ldbestrictlycontrolled.Itisalsostronglysuggestedthatthewholeassayisperformedbythesameoperatorfromthebeginningtotheend.

WehavethisCYP2C18Elisakitforhuman.

Youcanreferencelinkofthekitasfollowing

仪器网-专业分析仪器服务平台,实验室仪器设备交易网,仪器行业专业网络宣传媒体。

相关热词：

等离子清洗机,反应釜,旋转蒸发仪,高精度温湿度计,露点仪,高效液相色谱仪价格,霉菌试验箱,跌落试验台,离子色谱仪价格,噪声计,高压灭菌器,集菌仪,接地电阻测试仪型号,柱温箱,旋涡混合仪,电热套,场强仪万能材料试验机价格,洗瓶机,匀浆机,耐候试验箱,熔融指数仪,透射电子显微镜。