Anti-P14ARF 抗体说明书

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【导读】Anti-P14ARF抗体说明书 Goat Anti-P14ARF抗体说明书CatalogNumber: RS-1174R Quantitysize: 0....

Anti-P14ARF抗体说明书

Goat Anti-P14ARF抗体说明书

CatalogNumber: RS-1174R Quantitysize: 0.1ml （dilutewithpH7.4 0.01MPBSorantibodydiluent） Anti-P14ARF抗体说明书Background:Thisgenegeneratesseveraltranscriptvariantswhichdifferintheirfirstexons.Atleastthreealternativelysplicedvariantsencodingdistinctproteinshavebeenreported,twoofwhichencodestructurallyrelatedisoformsknowntofunctionasinhibitorsofCDK4kinase.Theremainingtranscriptincludesanalternatefirstexonlocated20Kbupstreamoftheremainderofthegene;thistranscriptcontainsanalternateopenreadingframe（ARF）thatspecifiesaproteinwhichisstructurallyunrelatedtotheproductsoftheothervariants.ThisARFproductfunctionsasastabilizerofthetumorsuppressorproteinp53asitcaninteractwith,andsequester,MDM1,aproteinresponsibleforthedegradationofp53.Inspiteofthestructuralandfunctionaldifferences,theCDKinhibitorisoformsandtheARFproductencodedbythisgene,throughtheregulatoryrolesofCDK4andp53incellcycleG1progression,shareacommonfunctionalityincellcycleG1control.Thisgeneisfrequentlymutatedordeletedinawidevarietyoftumors,andisknowntobeanimportanttumorsuppressorgene.CDKN2AAnti-P14ARF抗体说明书pecificity: – Anti-P14ARFisagoatmonoclonalantibodyunconjugated – specificforP14ARFofrabbit – useforwesternblotting,elisa,immunoprecipitationandimmunohistochemistry– ProteinGaffinitychromatographypurification,purity:>95%– Isotype: IgG– molwt: 12.5kDa Application: – Westernblotting1:100-500 – Immunohistochemistry1:100-500 – ELISA1:500-1000 – Optimalworkingdilutionsmustbedeterminedbytheenduser. Anti-P14ARF抗体说明书Storage: Storeat-20℃foroneyear.Avoidrepeatedfreeze/thawcycles.The lyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreater thanayearwhenkeptat-20℃.WhenreconstitutedinsterilepH7.40.01MPBSordiluent ofantibody,theantibodyisstableforatleastsixweeksat2-4℃ ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforusein human,therapeuticordiagnosticapplications.

2004-07-13 09:31:27

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