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仪器/ 产品中心/ 色谱柱/ 保护柱/ 保护柱套/ Dionex Acclaim PolarAdvantage LC Column 反相液相色谱柱
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Dionex Acclaim PolarAdvantage LC Column 反相液相色谱柱

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Acclaim PolarAdvantage II Reversed-Phase LC Column


Complementary Selectivity and Enhanced Hydrolytic Stability

Acclaim? PolarAdvantage II (PA2) columns feature a patented bonding chemistry that provides enhanced hydrolytic stability from pH 1.5–10 and allows resolution of a wide variety of polar and nonpolar analytes. The Acclaim PA2 is compatible with 100% aqueous phases and exhibits high reversed-phase capacity, with selectivity complementary to conventional C18 columns such as the Acclaim 120 C18. As with all Acclaim columns, the Acclaim PA2 is LC/MS-compatible.

  • Single-run analysis of polar and nonpolar analytes
  • Hydrolytically stable (pH 1.5–10)
  • Rugged column with high efficiency, symmetrical peak shapes even with basic and acidic compounds
  • Compatible with 100% aqueous phases
  • High reversed-phase capacity with selectivity complementary to conventional C18 columns

Acclaim PA2 columns are compatible with 100% aqueous phases, overcoming many of the limitations of conventional C8 and C18 reversed-phase columns. Conventional reversed-phase columns are unstable under these conditions and rapidly lose their ability to retain analytes.

Acclaim PA2 columns are available both in the 3- and 5-μm particle sizes and 4.6-, 3.0, and 2.1 mm- diameters, with and average pore diameter of 120?. Acclaim PA2 is also available in 2.2 ?m RSLC format (see Acclaim Rapid Separation LC (RSLC) Column section for more details). Acclaim PA2 may be used for some LC/MS applications. 

Highly Stable Under Wide pH Range

The proprietary bonding of the Acclaim PA2 column resists hydrolytic attack by protecting the bonded phase at low pH. This column is highly stable with low pH.

In addition to low pH stability, although it is common to analyze basic compounds under high-pH conditions to reduce peak tai领, most polar-embedded phases are even less hydrolytically stable than conventional C18 columns. The Acclaim PA2 is specifically designed to withstand high pH conditions, making it a good choice for the separation of both basic and acidic analytes.

Acclaim? PA2: A Polar-Embedded Reversed-Phase Column with Selectivity Complementary to Conventional C18 Columns  Specifications
pH range1.5–10
End-cappedYes
Metal impurity (Na, Fe, Al)<10.0 ppm Total Metal
<3.0 ppm Individual Metal
Pore volume0.9–1.0 mL/g
Average pore diameter120 ?
Surface area300 m2/g
Temperature< 60 oC
Starting materialUltrapure silica
Particle size3 and 5  ?m
Particle shapeSpherical
Particle size distribution (40/90)<1.3
Total carbon content17%
Carbon chain lengthC18
Analytical Columns
Acclaim 120, PA2, 3?m Analytical (2.1 x 50mm)077999
Acclaim 120, PA2, 3?m Analytical (2.1 x 100mm)077998
Acclaim PA2, 3 ?m Analytical, (2.1 x 150 mm)063187
Acclaim 120, PA2, 3?m Analytical (2.1 x 250mm)077997
Acclaim PA2, 3?m Analytical, (3.0 x 50mm)068973
Acclaim 120, PA2, 3?m Analytical (3.0 x 100mm)078000
Acclaim PA2, 3 ?m Analyitcal (3.0 x 150 mm)063705
Acclaim PA2, 3 ?m, Analytical, (3.0 x 250 mm)070080
Acclaim PA2, 3 ?m Analytical, (4.6 x 50 mm)063189
Acclaim 120, PA2, 3?m Analytical (4.6 x 100mm)078001
Acclaim PA2, 3 ?m Analytical, (4.6 x 150 mm)063191
Acclaim PA2, 5 ?m Analytical, (4.6 x 150 mm)063197
Acclaim PA2, 5 ?m Analytical, (4.6 x 250 mm)063199
Guard Columns
Acclaim PA2, 5 ?m Guard Cartridges, (4.6 x 10 mm), 2 ea (use V-2 Holder)069699
Acclaim PA2, 5?m Guard Cartridges (3 x 10mm), 2ea, requires holder 069580071985
Acclaim PA2, 5?m Guard Cartridges, (2.1 x 10mm), 2 ea; requires holder 069580069692
Acclaim SST Guard Cartridge Holder V-2069580
Acclaim Guard Kit (Holder and coupler) V-2069707
Guard to Analytical Column Coupler V-2074188
Acclaim RSLC PA2 Analytical Columns
Acclaim RSLC PA2, 3 ?m Analytical (3.0 x 33 mm)066276
Acclaim PA2, 3?m Analytical, (3.0 x 50mm)068973
Acclaim RSLC PA2, 3 ?m Analytical (3.0 x 75 mm)066277
Acclaim PA2, 3 ?m Analyitcal (3.0 x 150 mm)063705
Acclaim RSLC PA2, 2.2?m Analytical, (2.1 x 30mm)071402
Acclaim RSLC PA2, 2.2?m Analytical (2.1 x 50 mm)068989
Acclaim RSLC PA2, 2.2?m Analytical (2.1 x 100 mm)068990
Acclaim RSLC PA2, 2.2?m Analytical, (2.1 x 150mm)071401
Acclaim RSLC PA2, 2.2?m Analytical (2.1 x 250mm)074814
Acclaim RSLC PA2, 2.2 ?m Analytical, (3.0 x 100mm)071607
Acclaim RSLC PA2, 2.2 ?m Analytical, (3.0 x 50mm)071608
Acclaim RSLC PA2, 2.2 ?m Analytical, (3.0 x 30mm)071609


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