仪器网

欢迎您: 免费注册 仪器双拼网址:www.yiqi.com
首页-资讯-资料-产品-求购-招标-品牌-展会-行业应用-社区-供应商手机版
官方微信
仪器网-专业分析仪器,检测仪器平台,实验室仪器设备交易网 产品导购
VIP企业会员服务升级
仪器/ 产品中心/ 色谱柱/ 保护柱/ 保护柱套/ Dionex ProPac IMAC Column 生物色谱柱
收藏  

Dionex ProPac IMAC Column 生物色谱柱

联系方式:李经理13916220072

联系我们时请说明在仪器网(www.yiqi.com)上看到的!

为您推荐
详细介绍

Analytical Column for Immobilized Metal Affinity Chromatography

The ProPac? IMAC-10 is an analytical HPLC column packed with 10 ?m, nonporous, polymeric beads that are coated with a hydrophilic layer, then grafted with poly(IDA) chains. The poly(IDA) grafts are converted to metal-containing nanoparticles when the column is charged with metal. It is these nanoparticles that act as IMAC interaction sites for individual proteins and provide the ProPac IMAC-10 with its high resolving power.

  • State-of-the-art technology for reusable columns with metal tailored specificity
  • Resolve target proteins using a single column in a high resolution gradient run
  • Retention control by imidazole concentration or pH gradient
  • High purity separations of metal-binding proteins
  • Wide range of metal-specific applications 

The ProPac IMAC-10 design maximizes the number of sterically accessible ligands for binding to proteins, ensuring that the proteins remain tightly bound during separation. It can be operated between pH 2–12 and is compatible with commonly used protein purification reagents (non-ionic detergents, reducing agents). It can be used under native or denaturing HPLC conditions 

The ProPac IMAC-10 is shipped free from any metal and ready to be charged with the metal of your choice. To avoid contact of the metal solution with your system, charge the IMAC-10 with a loading column (an empty column body you fill with your metal solution, then place between the pump and the IMAC-10 column).


ProPac IMAC Columns Specifications
Maximum Operating Pressure<3000 psi (21 MPa)
Temperature Range<60oC
Recommended Temperature:  Controlled ambient
Typical Operating Conditions<600 psi at 1.0 mL/min (4 × 250mm)
Recommended flow rate: 1.0 mL/min
Phase CompatibilitypH 2–12, Compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Resin Compostion10 ?m diameter nonporous polystyrene divinylbenzene substrate (55% crosslinking) with poly(IDA) grafts.
Metal Ion Capacity (?mol Cu) >80 mmol Cu (4 × 250 mm analytical column)
Chelating MetalsNi, Fe, Cu, others
Analytical Column Dimensions1 × 50 mm, 2 × 50 mm, 4 × 50 mm, 9 × 50 mm
2 × 250 mm, 4 × 250 mm, 9 × 250 mm, 22 × 250 mm
Loading Column Dimensions4 × 50 mm, 9 × 50 mm, 9 × 250 mm



Analytical Columns
ProPac IMAC-10 Column (1 x 50 mm)063617
ProPac IMAC-10 Column (2 x 50 mm)063272
ProPac IMAC-10 Column (4 x 50 mm)063276
ProPac IMAC-10 Column (9 x 50 mm)063615
ProPac IMAC-10 Column (2 x 250 mm)063274
ProPac IMAC-10 Column (4 x 250 mm)063278
ProPac IMAC-10 Column (9 x 250 mm)063280
ProPac IMAC-10 Column (22 x 250 mm)063282
Accessories
IMAC Loading Column (4 x 50 mm)063667
IMAC Loading Column (9 x 50 mm)063710
IMAC Loading Column (9 x 250 mm)063718
主要用途
戴安公司的快速溶剂萃取仪15分钟之内仅用15毫升溶剂既可完成对10g样品的萃取,该技术已在环境、药物、食品和聚合物工业等领域得到广泛应用。
厂商相关其他产品
X您尚未登录
账号登录
X您尚未登录
手机动态密码登录
X您尚未登录
扫码登录
在线留言
官方微信

仪器网微信服务号

扫码获取最新信息


仪器网官方订阅号

扫码获取最新信息

在线客服

咨询客服

在线客服
工作日:  9:00-18:00
联系客服 企业专属客服
电话客服:  400-822-6768
工作日:  9:00-18:00
订阅商机

仪采招微信公众号

采购信息一键获取海量商机轻松掌控