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Dionex DNAPac PA100 Nucleic Acid Column 生物色谱柱
- 品牌:美国戴安
- 货号:
- 产地:美国
- 供应商报价: 面议
- 上海希言科学仪器有限公司 更新时间:2024-04-23 17:09:46
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- 同类产品生物耗材(5878件)
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- 详细介绍
DNAPac PA100 Nucleic Acid Column
For n, n-1 Resolution of Single-Stranded Oligonucleotides
The DNAPac? PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13 μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized MicroBeads?. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations.
- Capable of n, n-1 resolution for oligonucleotides
- Resolves oligonucleotides with secondary structures
- Compatible with solvent, high pH, and high temperatures
- Analyzes phosphorothioate-based clinical samples
- Provides easy scale-up
The MicroBead resin used in DNAPac columns provides high-resolution separations to ensure maximum peak purity, and makes linear scale-up of your separation easy. For more complex separations, the polymeric properties of the DNAPac PA100 allows great flexibility to select ideal conditions, including high pH, high temperature, or 超tropic agents.
Synthetic oligonucleotides can be screened for production yield and failure sequences using the DNAPac PA100. Unit-base resolution of synthetic oligonucleotides to 60 bases and beyond has been demonstrated. The DNAPac PA100 can even be operated under denaturing conditions. Either high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5) can be used to eliminate hydrogen bonding, allowing resolution of problem sequences such as self-complementary sequences or poly-G stretches.
Separates RNA / Double-Stranded DNA
The DNAPac PA100 is ideally suited for the purification and analysis of synthetic RNA. Failure sequences are easily separated from the full-length product. Double-stranded DNA, such as plasmids, or restriction fragments are also separated.
Analytical separations on the 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. The loading capacity of the DNAPac PA100 (4 × 250 mm) column is from 1 to 100 μg. Sca领 the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.
DNAPac? PA100 for n, n - 1 Resolution of Single-Stranded Oligonucleotides Specifications Temperature range 4–90 °C Typical operating conditions 2400 psi at 1.0 mL/min (analytical and guard columns)
Recommended flow rate: 1.0 mL/minphase compatibility pH 2–12.5; 100% compatible with common organic solvents.
Ionic form eluents: chloride, acetate, or bromide in lithium, sodium, or ammonium forms.Resin composition 13 μm-diameter nonporous substrate (55% cross-linking) agglomerated with a 100 nm MicroBead alkyl quaternary ammonium functionalized latex (5% cross-linked). Anion-exchange capacity ~40 μeq/column (4 × 250 mm analytical column) Column construction PEEK? with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.Dimensions DNAPac PA100 Analytical Column:
2 × 250 mm
DNAPac PA100 Guard Column:
2 × 50 mm
DNAPac PA100 Analytical Column:
4 × 250 mm
DNAPac PA100 Guard Column:
4 × 50 mm
DNAPac PA100 Semipreparative Column:
9 × 250 mm
DNAPac PA100 Semipreparative Column:
22 × 250 mm
DNAPac PA100 Semipreparative Guard Column:
22 × 50 mmMaximum operating pressure 4000 psi (27.9 MPa) Analytical Columns DNAPac PA-100 Analytical Column (4 x 250 mm) 043010 DNAPac PA-100 Semi-Preparative Column (9 x 250 mm) 043011 DNAPac PA-100 Semi-Preparative Column (22 x 250 mm) SP2091 FAST-Cartridge, DNAPac PA-100 (2 x 10 mm), Pkg of 150 SP4008 FAST-Cartridge, DNAPac PA-100 (2 x 10 mm), Pkg of 600 SP3229 DNA Pac PA-100 Analytical Column (2 x 250 mm) SP3686 DNAPac PA-100 Column (22 x 50 mm) SP4513 FAST-Cartridge, DNAPac PA-100 (2 x 10 mm), Pkg of 10 SP4757 Guard Columns DNAPac PA-100 Guard Column (2 x 50 mm) SP4016 DNAPac PA-100 Guard Column (4 x 50 mm) 043018 - 主要用途
- 戴安公司的快速溶剂萃取仪15分钟之内仅用15毫升溶剂既可完成对10g样品的萃取,该技术已在环境、药物、食品和聚合物工业等领域得到广泛应用。