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美国戴安Dionex ProPac SAX and WAX Protein Column
- 品牌:美国戴安
- 货号:
- 产地:美国
- 供应商报价: 面议
- 上海希言科学仪器有限公司 更新时间:2024-03-27 13:33:47
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- 同类产品生物耗材(5878件)
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- 详细介绍
美国戴安Dionex ProPac SAX and WAX Protein Column 生物色谱柱
High-Resolution, Strong and Weak Anion-Exchange Columns for the Separation of Proteins
The ProPac® SAX-10 is a nonporous, strong anion-exchange column (quaternary amine functional group). The ProPac WAX-10 column is a weak anion exchanger (tertiary amine functional group). Both are based on a nonporous core particle that provides pressure stability. A hydrophilic layer prevents unwanted secondary interactions and a grafted anion exchange surface provides pH control, selectivity, and fast mass transfer for high efficiency separation, as well as moderate capacity.
- Unequalled resolution
- High-efficiency peaks with excellent recovery
- Higher capacity than other columns based on nonporous particles
- Useful for characterization and quality control assessment of closely-related protein variants
- Supports separation of proteins that differ by as little as one amino acid residue
- Neutral hydrophilic coat that eliminates protein-resin hydrophobic interactions
- Superior lot-to-lot and column-to-column reproducibility
ProPac SAX-10 and WAX-10 columns can resolve protein isoforms that differ by as little as one charged residue. Differences in selectivity between the WAX-10 and SAX-10 provide flexibility in maximizing resolution of closely related species.
All ProPac columns are manufactured and tested under the strictest specifications, resulting in unmatched column-to-column and lot-to-lot reproducibility. Dionex also offers these columns as lot select packages, containing three columns from one lot or three columns from three different lots, to assist quality control scientists in method validation.
ProPac SAX-10 Strong Anion-Exchange Column for the High-Resolution Separation of Protein Isoforms Specifications Maximum operating pressure 3000 psi (21 MPa) Maximum operating conditions 60 °C
Recommended operating temperature: <40 °CTypical operating conditions UV detection at 1 mL/min
Recommended flow rate: 1 mL/minphase compatibility pH 2–12; 80% compatible with acetonitrile, acetone, methanol.
Compatible with nonionic and zwitterionic detergents.
Incompatible with anionic detergents.Resin composition 10-?m-diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the quaternary ammonium functional groups. Anion-exchange capacity < 100 ?g protein on-column, depending upon the protein
(4 × 250 mm analytical column)Column construction PEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.ProPac WAX-10 Weak Anion-Exchange Column for the High-Resolution Analysis of Protein Variants Specifications Maximum operating pressure 3000 psi (21 MPa) Maximum operating temperature 60 °C
Recommended operating temperature: <40 °CTypical operating conditions UV detection at 1 mL/min
Recommended flow rate: 1 mL/minphase compatibility pH 2–12; 80% compatible with acetonitrile, acetone, methanol.
Compatible with nonionic and switterionic detergents.
Incompatible with anionic detergents.Resin composition 10-?m-diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the tertiary amine functional groups. Anion-exchange capacity < 100 ?g protein on-column, depending upon the protein
(4 × 250 mm analytical column)Column construction PEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.SAX Analytical Columns ProPac SAX-10 Analytical Column (2 x 250 mm) 063448 ProPac SAX-10 Analytical Column (4 x 250 mm) 054997 ProPac SAX-10HT Analytical Column (4 x 50 mm) 078990 ProPac SAX-10 Lot Select Column Set (4 x 250 mm) (1 Lot of Resin) SP5729 ProPac SAX-10 Lot Select Column Set (4 x 250 mm) (3 Lots of Resin) SP5730 ProPac SAX-10 Semi-Preparative Column (9 x 250 mm) 063703 ProPac SAX-10 Semi-Preparative Column (22 x 250 mm) SP5594 WAX Analytical Columns ProPac WAX-10 Analytical Column (2 x 250 mm) 063464 ProPac WAX-10 Analytical Column (4 x 250 mm) 054999 ProPac WAX-10 Lot Select Column Set (4 x 250 mm) (1 Lot of Resin) SP5731 ProPac WAX-10 Lot Select Column Set (4 x 250 mm) (3 Lots of Resin) SP5732 ProPac WAX-10 Semi-Preparative Column (9 x 250 mm) 063707 ProPac WAX-10 Semi-Preparative Column (22 x 250 mm) SP5598 WAX Guard Columns ProPac WAX-10G Guard Column (2 x 50 mm) 063470 ProPac WAX-10G Guard Column (4 x 50 mm) 055150 SAX Guard Columns ProPac SAX-10G Guard Column (2 x 50 mm) 063454 ProPac SAX-10G Guard Column (4 x 50 mm) 054998 - 主要用途
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