DLD Diagnostika 2017价格表

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世界ding级实验材料供应商BioCheck正式授权上海起发为其ZG代理，BioCheck在一直是行业的标杆,一直为广大科研客户提供Z为优质的产品和服务,上海起发一直秉承为ZG科研客户带来**的产品，**的服务，签约BioCheck就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们Zda的收获BioCheckZG代理,BioCheck上海代理，BioCheck北京代理，BioCheck广东代理，BioCheck江苏代理BioCheck湖北代理,BioCheck天津,BioCheck黑龙江代理,BioCheck内蒙古代理,BioCheck吉林代理,BioCheck福建代理,BioCheck江苏代理,BioCheck浙江代理,BioCheck四川代理BIOCHECK公司由创始人Dr.JohnChen,Medix创立,是一家抓也提供肿瘤标志物，心肌标志物，激素类Z高性价比的抗体的公司。简要原理利用竞争酶联免疫方法，预先在微孔中包被羊抗兔抗体，实验时先后加入氯霉素标准品或待测样本，氯霉素酶标抗原和兔抗氯霉素抗体。经过室温温育，反应液中的兔抗氯霉素抗体与微孔板上的羊抗兔抗体结合，待测样品中的抗原与氯霉素酶标抗原竞争微孔板上的兔抗氯霉素抗体。洗涤后，没有与抗体结合的待测样品中的抗原或酶标抗原被洗去，再加入反应底物，结合的酶标抗原的酶将底物转化为蓝色产物，加入终止液后颜色由蓝色变为黄色。反应完成后，样品中氯霉素含量越多，反应呈色就越浅；反之，样品中氯霉素含量越少，则呈色越深。利用标准曲线可计算出样品中氯霉素含量。技术参数检测蜂蜜、蛋类、牛奶、奶粉、水产品、动物组织(肌肉、肝脏等)、饲料、血清、血浆及尿液样本中存在的氯霉素，定量限可达0.025ppb。No.样品检测下限1蜂蜜0.02ppb(0.02ng/g)2蛋类0.02ppb(0.02ng/g)3牛奶0.002ppb(0.002ng/ml)4奶粉0.012ppb(0.012ng/g)5虾，鱼及肉类0.08ppb(0.08ng/g)6饲料0.08ppb(0.08ng/g)7血清/血浆0.02ppb(0.02ng/ml)8尿液0.04ppb(0.04ng/ml)交叉反应名称百分比氯霉素碱0.4%甲基氯霉素<0.04%回收率No.样品回收率1蜂蜜70%~110%2牛奶90%~130%3蛋，虾，鱼及肉类95%~120%4饲料95%~120%5血清/血浆90%~120%6尿液1**%~130%PD-1ENZYMEIMMUNOASSAYTESTKITCatalogNumber:BC-1301EnzymeImmunoassayfortheQuantitativeDeterminationofPD-1ConcentrationinHumanSerumandPlasmaFORRESEARCHUSEONLYNotforuseindiagnosticproceduresINTRODUCTIONProgrammedcelldeathprotein1,(PD-1,CD279,PDCD1),isacellsurfacereceptorthatiscriticalfortheregulationofTcellinflammatoryactivityandmaintainsperipheraltoleranceintheimmunesystem(1).WithanextracellularIgVdomain,atransmembranedomain,andanintracellulartailwithtwophosphorylationsites(SHP-1andSHP-2),PD-1isabletointeractwithligandsPD-L1andPD-L2todown-regulatetheimmunesystemandsuppressTcellactivity(2).Uponantigenrecognition,anactivatedTcellexpressesPD-1onitssurfaceandproducesinterferons,whichinducesexpressionofPD-L1,therebypromotingapoptosisorcelldeath.(3)Howeverwhenhijackedbytumorcells,aberrantexpressionofPD-1ligandsinhibitsimmune-modulatoryTcellactivation,leadingtodiseaseprogression(4).ElevatedlevelsofPD-1inserumandplasmahavebeenassociatedwithrheumatoidarthritisandskinsclerosis(5,6).Also,PD-1ispredominantlyexpressedbytumorinfiltratingTcells(7).FurtherresearchimpliesthatusingmonoclonalantibodiestotargetthePD-1immunologiccheckpointhascontributedtobreakthroughprogressinunderstandingandtreatingcancerssuchasmelanomaandothervariousnon-smallcelllungcancer(4).PRINCIPLEOFTHEASSAYThePD-1ELISAisbasedontheprincipleofasolidphaseenzyme-linkedimmunosorbentassay.TheassaysystemutilizesauniquemonoclonalantibodypairdirectedagainstadistinctantigenicdeterminantonthePD-1molecule.Onemousemonoclonalanti-PD-1antibodyisusedforsolidphaseimmobilization(onthemicrotiterwells).Anothermousemonoclonalanti-PD-1antibodyconjugatedtohorseradishperoxidase(HRP)isintheenzymeconjugatesolution.Thetestsamplesareallowedtoreactsequentiallywiththetwoantibodies,resultinginthePD-1moleculestobesandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Aftertwoseparate60-minuteincubationstepsatroomtemperature,thewellsarerinsedwithWashBuffertoremoveunboundlabeledantibodies.TMBReagentisaddedandincubatedfor30minutesunderdarkconditions,resultinginthedevelopmentofabluecolor.ThecolordevelopmentisstoppedwiththeadditionofStopSolution,changingthecolortoyellow.TheconcentrationofPD-1isdirectlyproportionaltothecolorintensityofthetestsamples.Absorbanceismeasuredspectrophotometricallyat450nm.REAGENTSANDMATERIALSPROVIDEDAntibody-CoatedWells(1plate,96wells)Microtiterwellscoatedwithmousemonoclonalanti-PD-150ng/mlPD-1Standard(0.5mL/vial)50ng/mLPD-1inphosphatebuffer-BSAsolutionwithpreservatives3.StandardandSampleDiluent(30mL/bottle,1bottle)Containsphosphatebuffer-BSAsolutionwithpreservativesEnzymeConjugateReagent(12mL/vial,1vial)Containsmousemonoclonalanti-PD-1conjugatedtohorseradishperoxidase20XWashBuffer(50mL/bottle,1bottle)PhosphatebufferwithdetergentsTMBReagent(11mL/bottle,1bottle)Containsone-stepTMBsolutionStopSolution(11mL/bottle,1bottle)Containsdilutedhydrochloricacid(1NHCl)STORAGECONDITIONSStoretheunopenedkitat2-8°Cuponreceiptandwhenitisnotinuse,untiltheexpirationshownonthekitlabel.Refertothepackagelabelfortheexpirationdate.Keepmicrotiterplateinasealedbagwithdesiccanttominimizeexposuretodampair.REAGENTPREPARATIONAllreagents首ldbeallowedtoreachroomtemperature(18-25°C)beforeuse.Foreachtestrun,prepareafreshstandardset.Dilute50ng/mLto10ng/mLwithStandard/Samplediluent.Preparetwo-foldserialdilutionsofthe10ng/mLStandardwithStandard/SampleDiluent:10ng/mL:0.12mLof50ng/mL+0.48mLofStandard/SampleDiluent5ng/mL:0.25mLof10ng/mL+0.25mLofStandard/SampleDiluent2.5ng/mL:0.25mLof5ng/mL+0.25mLofStandard/SampleDiluent1.25ng/mL:0.25mLof2.5ng/ml+0.25mLofStandard/SampleDiluent0.625ng/mL:0.25mLof1.25ng/mL+0.25mLofStandard/SampleDiluent0.313ng/mL:0.25mLof0.625ng/mL+0.25mLofStandard/SampleDiluent0.156ng/mL:0.25mLof0.313ng/mL+0.25mLofStandardDiluent0ng/mL:0.25mLofStandardDiluentPatientsamplesneedtobediluted4-foldpriortouse.Prepareaseriesofsmalltubes(i.e.,1.5mLmicrocentrifugetubes)andmix60Lofserumwith180LStandard/SampleDiluent.WorkingWashBuffer:Preparationof1XWashBufferfrom20XStock.Add50mLof20XWashBufferStockto950mLofDIH2O.TheWorkingWashBufferisstableat2-8°Cfor30days.NOTE:Anycrystalsthatmaybepresentduetohighsaltconcentrationmustberedissolvedatroomtemperaturebeforemakingthedilution.ASSAYPROCEDUREPrepareStandards.SeeReagentPreparation.Dilutesamples1:4dilution.SeeReagentPreparation.Securethedesirednumberofcoatedwellsintheholder.Dispense100mLofPD-1standards,andDILUTEDspecimensintotheappropriatewells.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300mLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLofPD-1WorkingEnzymeConjugateReagentintoeachwell.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300uLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLTMBsolutionintoeachwell.Incubatefor30minutesatroomtemperature(18-25°C).Stopthereactionbyadding100mLofStopSolutionintoeachwell.Gentlymixfor30seconds.Itisimportanttomakesurethatallthebluecolorchangestoyellowcolorcompletely.Readabsorbanceat450nmwithamicrotiterwellreaderwithin15minutes.CALCULATIONOFRESULTSCalculatethemeanabsorbancevalue(OD450)foreachsetofreferencestandards,controlsandsamples.Constructastandardcurvebyplottingthemeanabsorbanceobtainedforeachreferencestandardagainstitsconcentrationinng/mlongraphpaper,withabsorbanceonthevertical(y)axisandconcentrationonthehorizontal(x)axis.ThecorrespondingconcentrationofPD-1(ng/mL)canbedeterminedfromthestandardcurveusingthemeanabsorbancevalueforeachsample.Dependingonexperienceand/ortheavailabilityofcomputercapability,othermethodsofdatareductionmaybeemployed.Theobtainedvaluesofthesamples首ldbemultipliedbythedilutionfactorof4toobtainPD-1resultsinng/ml.EXAMPLEOFSTANDARDCURVEResultsofatypicalstandardrunwithabsorbencyreadingsat450nmshownontheYaxisagainstPD-1concentrationsshownontheXaxis.NOTE:Thisstandardcurveisforthepurposeofillustrationonly,and首ldnotbeusedtocalculateunknowns.Eachlaboratorymustgenerateitsowndataandstandardcurveineachexperiment.PD-1Absorbance(ng/ml)(450nm)00.0890.1560.1380.3130.1900.6250.2841.250.4812.50.85151.581102.776nm32.5(4502Absorbance1.510.500246810PD-1Conc.(ng/ml)PERFORMANCECHARACTERISTICSSensitivityTheminimumdetectableconcentrationofthePD-1ELISAassayasmeasuredby2SDfromthemeanofazerostandardisestimatedtobe0.15ng/ml.PrecisionIntra-AssayPrecisionWithin-runprecisionwasdeterminedbyreplicatedeterminationsofthreedifferentsamplesinoneassay.Within-assayvariabilityisshownbelow:Sample123#Replicates242424MeanPD-1(ng/mL)8.413.222.9S.D.0.61.00.9C.V.(%)7.0%7.2%3.8%.2Inter-AssayPrecisionBetween-runprecisionwasdeterminedbyreplicatemeasurementsofthreedifferentsamplesoveraseriesofindividuallycalibratedassays.Between-assayvariabilityisshownbelow:Sample123#Replicates202020MeanPD-1(ng/mL)9.113.525.3S.D.0.81.10.9C.V.(%)9.2%7.93.7RecoveryandLinearityStudiesRecoverySampleswerespikedwithknownPD-1levelsandassayedinduplicate.Themeanrecoverywas105.3%.PAIREXPECTEDOBSERVED%RECOVERYNUMBER[PD-1][PD-1](ng/mL)(ng/mL)188.8110.0%212.513.1104.8%32525.3101.2%LinearityThreesampleswereseriallydilutedtodeterminelinearity.Themeanrecoverywas99.6%.#DilutionExpectedObservedConc.(ng/ml)Conc.(ng/ml)%Expected1.1:210.1114.8%1:48.88.8N/A1:87.888.6%Mean=101.7%2.1:214.5110.6%1:413.113.1N/A1:811.688.5%Mean=99.6%3.1:226.5104.7%1:425.325.3N/A1:823.090.0%Mean=97.4%REFERENCESFife,B.T.,&Pauken,K.E.(2011).TheroleofthePD-1pathwayinautoimmunityandperipheraltolerance.AnnalsoftheNewYorkAcademyofSciences,1217(1),45-59.Riella,L.V.,Paterson,A.M.,Sharpe,A.H.,&Chandraker,A.(2012).RoleofthePD-1PathwayintheImmuneResponse.AmericanJournalofTransplantation:OfficialJournaloftheAmericanSocietyofTransplantationandtheAmericanSocietyofTransplantSurgeons,12(10),25752587.Zak,Krzysztof&Kitel,Radosaw&Przetocka,Sara&Golik,Przemysaw&Guzik,Katarzyna&Musielak,Bogdan&Dm领,Alexander&Dubin,Grzegorz&Holak,Tad.(2015).StructureoftheComplexofHumanProgrammedDeath1,PD-1,andItsLigandPD-L1.Structure.23..10.1016/j.str.2015.09.010.ZoranGatalica,CarrieSnyder,ToddManey,AnatoleGhazalpour,DanielHolterman,NianqingXiao,PeggyOverberg,IngaRose,GargiD.Basu,SemirVranic,HenryT.Lynch,DanielD.VonHoffandOmidHamid.ProgrammedCellDeath1(PD-1)andItsLigand(PD-L1)inCommonCancersandTheirCorrelationwithMolecularCancerType.CancerEpidemiolBiomarkersPrev.December12014(23)(12)2965-2970.Greisen,S.,Rasmussen,T.,Stengaard-Pedersen,K.,Hetland,M.,Hrslev-Petersen,K.,Hvid,M.,&Deleuran,B.(2013).Increasedsolubleprogrammeddeath-1(sPD-1)isassociatedwithdiseaseactivityandradiographicprogressioninearlyrheumatoidarthritis.ScandinavianJournalofRheumatology,43(2),101-108.Yanaba,K.,Hayashi,M.,Yoshihara,Y.,&Nakagawa,H.(2016).Serumlevelsofsolubleprogrammeddeath-1andprogrammeddeathligand-1insystemicsclerosis:Associationwithextentofskinsclerosis.TheJournalofDermatology,43(8),954-957.Ahmadzadeh,M.,Johnson,L.A.,Heemskerk,B.,Wunderlich,J.R.,Dudley,M.E.,White,D.E.,&Rosenberg,S.A.(2009).TumorantigenspecificCD8TcellsinfiltratingthetumorexpresshighlevelsofPD-1andarefunctionallyimpaired.Blood,114(8),15371544.部分产品Zxin报价如下：货号品名规格价格品牌BC-1009AFPEIAKitkit3980BioCheckBC-1011CEAEIAKitkit4016BioCheckBC-1013CA125EIAKitkit4970BioCheckBC-1015CA15-3EIAKitkit4970BioCheckBC-1017CA19-9EIAKitkit4970BioCheckBC-1019PSAEIAKit*kit3962BioCheckBC-1021FreePSAEIAKit*kit4196BioCheckBC-1023Freeb-hCGEIAKitkit4196BioCheckBC-1061b-2MicroglobulinEIAKit§kit4250BioCheckBC-1001TSHEIAKit§kit3800BioCheckBC-1003U-TSH(S-TSH)EIAKit§kit3800BioCheckBC-1005T3EIAKit§kit3656BioCheckBC-1006FreeT3EIAKit§kit3890BioCheckBC-1007T4EIAKit§kit3656BioCheckBC-1008FreeT4EIAKitkit3890BioCheckBC-1081RubellaIgGEIAKit*kit4106BioCheckBC-1083RubellaIgMEIAKit*kit4196BioCheckBC-1085ToxoIgGEIAKit*kit3980BioCheckBC-1087ToxoIgMEIAKit*kit4070BioCheckBC-1089CMVIgGEIAKit*kit3980BioCheckBC-1091CMVIgMEIAKit*kit4070BioCheckBC-1093HSVIIgGEIAKitkit3980BioCheckBC-1095HSVIIgMEIAKitkit4196BioCheckBC-1097HSVIIIgGEIAKitkit3980BioCheckBC-1099HSVIIIgMEIAKitkit4070BioCheckBC-1051H.pyloriIgG(Qualitative)EIAKitkit4016BioCheckBC-1052H.pyloriIgG(Quantitative)EIAKitkit4106BioCheckBC-1053H.pyloriIgM(Qualitative)EIAKitkit4286BioCheckBC-1027hCGEIAKit§kit3800BioCheckBC-1029FSHEIAKit§kit3800BioCheckBC-1031LHEIAKit§kit3800BioCheckBC-1037ProlactinEIAKit§kit3800BioCheckBC-1025HumanFerritinEIAKit§kit3800BioCheckBC-1033HGHEIAKit§kit3800BioCheckBC-1035IgEEIAKit§kit3800BioCheckBC-1203AdiponectinEIAKit*kit7580BioCheckBC-1105TroponinIEIAKit§kit5330BioCheckBC-1117MyoglobinEIAKit§kit4250BioCheckBC-1119HS-CRPEIAKit§kit4250BioCheckBC-1121CK-MBEIAKit*kit6680BioCheckBC-1129MPOEIAKit*kit8480BioCheckBC-1111EstradiolEIAKit§kit4250BioCheckBC-1113ProgesteroneEIAKit§kit4250BioCheckBC-1115TestosteroneEIAKit§kit4250BioCheck我们公司Zda优势是强大的采购，1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，2：货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，3：提供加急服务，一般1-2周到货，超过时限加急费全免4：价格公道，绝大部分价格有优势，当然不能保证1**%产品都有价格优势，5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大交大复旦，中山等100多所大学，ROCHE，阿斯利康，国药，fisher等500多家公司6：我们还是santa,AdvancedBiotechnologiesInc;AthensResearch&Technology,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.FormuMaxScientific,Inc;Genebridege;GlycotopeBiotechnologyGmbH;iduron;InnovativeResearchofAmerica；Ludger；neuroprobe;omicronbio;Polysciences;prospecbi;QA-BIO；quickzyme；RESEARCHDIETS,INC；sterlitech；sysy；TriLinkBioTechnologies,Inc；BIOCHECKINC-biochem;zyagen;......几十家国外公司授权代理7：我们还从事invitrogen，qiagen;abcam;sigma;neb;roche;merck;rnd;BD;GE;pierce;BioLegend....等批发
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