pTA-Luc载体说明书
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pIκB-EGFP型号载体名称出品公司载体用途VXC0435pIκB-EGFPClontech信号通路报告载体Description:ThepIκB-EGFPVectorencodestheIκB-EGFPSigna领Probe,whichisafusionofenhancedgreenfluorescentprotein(EGFP)andIκB.IκBisaninhibitorofNFκB,atranscriptionfactorinvolvedintheimmuneresponseandinflammatorydiseases.WhentheNFκBpathwayisinactive,IκBandNFκBexistasaninactivecomplexinthecytosol.Uponstimulation,IκBisdegraded.IncellstransfectedwithpIκB-EGFP,degradationoftheIκB-EGFPfusionproteinisobservedasadecreaseinEGFPfluorescence(1).TheIκB-EGFPSigna领Probeisconstitutivelyexpressedandresidesinthecytosol.EGFPisared-shifted,humancodon-optimizedvariantofGFP(26)thathasbeenengineeredforbrighterfluorescenceandhigherexpressioninmammaliancells.Itsexcitationmaximumis488nmandemissionmaximumis509nm.FormoreinformationonthepropertiesofEGFP,pleaserefertotheBDLivingColorsUserManual(PT2040-1)includedwiththevector.ExpressionoftheIκB-EGFPisdrivenbythehumanCMVimmediate-earlypromoter.TheSV40poly-Asequencedirectsproperprocessingofthe3'endofthefusionconstruct.ThevectorbackbonealsocontainsanSV40originforreplicationinmammaliancellsexpressingtheSV40T-antigen.Aneomycinresistancecassette(Neor)allowskanamycinselectioninE.coliandneomycin(G418)selectionineukaryoticcells.ThiscassetteconsistsoftheSV40earlypromoter,theneomycin/kanamycinresistancegeneofTn5,andpoly-AsignalsfromtheHerpessimplexvirusthymidinekinase(HSVTK)gene.ThevectorbackbonealsoprovidesapUCoriginofreplicationforpropagationinE.coliandanf1originforsingle-strandedDNAproduction.质粒图谱:上海起福生物科技有限公司联系人:杨建辉400电话:4006551678办公电话:021-50724187传真:021-50724961*8006手机:15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址:上海市浦东新区绣川路561号1102室
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