RNAi-Ready pSIREN-Retro Q-ZsGreen
- 上传人: 上海起发实验试剂有限公司 |大小:94.39KB|浏览:1985次|时间:2018-09-29
- 文档简介
- 您可能感兴趣的资料
-
AmbionRNAi载体,RNAi载体,pSilencer5.1-U6Retro载体pSilencer5.1
-
RNAi的生物学功能
-
RNAi技术及其在作物遗传育种中的应用
-
RNAi的特点与生物学功能
-
士锋RNAi的应用
-
pSUPER.Retro-GFP/Neo载体说明书型号载体名称出品公司载体用途VRO0352pSUPER.R
-
pSuperretropuro载体说明书型号载体名称出品公司载体用途VRO0353pSUPER.retro.
-
RNAi载体,pSilencer5.1-H1Retro载体pSilencer5.1-H1Retro型号载体名
-
RNAi载体,RNAi-ReadypSIREN-RetroQ-DsRed-Express载体RNAi-Rea
-
RNAi载体,RNAi-ReadypSIREN-RetroQ载体,ClontechRNAi-ReadypSI
RNAi载体,RNAi-ReadypSIREN-RetroQ-ZsGreen载体RNAi-ReadypSIREN-RetroQ-ZsGreen型号载体名称出品公司载体用途VRC0347RNAi-ReadypSIREN-RetroQ-ZsGreenClontechRNAi载体产品参数:Promoter:HumanU6Reporter:ZsGreenSelection:prokaryoticampicillinReplication:prokaryoticColE1ori(lowcopy),viralSV40ori载体抗性:氨苄青霉素(Ampicillin)载体描述:RNAi-ReadypSIREN-RetroQ-ZsGreenderivesfromouroriginalgenesilencingretroviralvectorRNAi-ReadypSIREN-RetroQ.pSIREN-RetroQ-ZsGreenisaself-inactivatingretroviralexpressionvectordesignedtoexpressasmallhairpinRNA(shRNA)usingthehumanU6promoter.ThisvectorisprovidedasalinearizedvectordigestedwithBamHIandEcoRI.ItisusedfortargetedgenesilencingwhenanannealedDNAoligonucleotideencodinganappropriateshRNAisligatedintothevector.ThepSIREN-RetroQ-ZsGreenconstructcanbetransfectedasaplasmidexpressionvector.Forgenerationofactiveretrovirusforinfectinghard-to-transfectcelllines,transfectthepSIREN-RetroQ-ZsGreenconstructintoaretroviralpackagingcelllineandcollecttheviralsupernatant.质粒图谱:
标签: