Super Script Ⅱ阴性逆转录酶,SuperScript II Reverse Transcriptase
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SuperScriptⅡ阴性逆转录酶,SuperScriptIIReverseTranscriptase英文名称:SuperScriptIIReverseTranscriptase级别:BR来源:PurifiedfromE.coliexpressingthepolgeneofM-MLV(1,2),mutagenizedtoreducetheRNaseHactivity浓度:200u/ul活力定义:OneunitofSuperScriptIIistheamountofenzymerequiredtoincorporate1nmoleofeoxyribonucleotideintoacid-precipitablematerialin10min.at37°Cusingpoly(A)oligo(dT)12-18astemplateprimer(3).UnitReactionConditions:50mMTris-HC1(pH8.3),40mMKC1,6mMMgC12,1mMDTT,0.5mM[3H]dTTP,0.1mMpoly(A),0.1mMoligo(dT)12-18,0.1mg/m1BSA,andenzymein50lfor10min.at37℃PerformanceandQualityTesting:SDS-PAGEpurity;endodeoxyribonuclease,exodeoxyribonuclease,andribonucleaseassays;andyieldandlengthofcDNAproductEnzymeFunction:RNAdependentDNAPolymeraseFinalProductSize:12.3kborlessNumberofReactions:10ReactionsOptimalReactionTemperature:42℃Sensitivity:MediumTemplate:ssRNA;ssDNA;RNA-DNAHybrid产品描述:SuperScriptIIReverseTranscriptase(RT)isanimprovedversionofSuperScriptRT.ItisaDNApolymerasethatsynthesizesacomplementaryDNAstrandfromsingle-strandedRNA,DNA,oranRNA:DNAhybrid.ThisenzymeisgeneticallyengineeredbytheintroductionofpointmutationsratherthanadeletionintheRNaseHactivecenter.LikeSuperScriptRT,SuperScriptIIRThasreducedRNaseHactivity.UnlikeSuperScriptRT,however,theselectivemutationswithintheRNaseHdomainmaintainfullpolymeraseactivity.ThisstructuralmodificationeliminatesdegradationofRNAmoleculesduringfirst-strandcDNAsynthesisandgivesSuperScriptIIRTsuperiorperformancecharacteristics,including:Greaterfirst-strandcDNAyields;Morefull-lengthcDNAsynthesis;Fullactivityat42℃性状:悬浮液用途:生化研究。保存:-20℃SuperScriptⅡ阴性逆转录酶,SuperScriptIIReverseTranscriptaseSuperScriptⅡ阴性逆转录酶,SuperScriptIIReverseTranscriptaseSuperScriptⅡ阴性逆转录酶,SuperScriptIIReverseTranscriptase
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