Properties of aqueous solutions of lentinan in the absence andpresence of zwitterionic surfactants
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Properties of aqueous solutions of lentinan in the a
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Morphologicalandconformationaltransitionsoflentinan(LT),aˇ-glucanextractedfromShiitakemush-rooms(Lentinulaedodes),wereinvestigatedatdifferentconcentrationsofaqueousNaOH,usingSmallAngleX-rayScattering(SAXS)technique.AtlowNaOH(aq)concentrationLTchainsareself-associatedandadoptthetriplehelixformwhereasathigherNaOHconcentrationsthepolymerchainsundergoatransitiontorandomcoilchains.Also,thepresenceoffractaldimensionswasobservedthroughanalysisoftheexponentialdecayofthescatteringintensityasafunctionofthescatteringangle.Inaddition,thelateralradiusofgyrationwasdeterminedforLTindifferentconcentrationsofNaOHsolution,indicatingarigidtriplehelixpresentasasmallrod-likestructure.InteractionsofLTwithtwozwitterionicsurfactantswereinvestigatedbysurfacetension,fluorescence,andstaticlightscatteringmeasurements.Experimen-taldatashowedthattheformationofLT(surfactant)complexesoccurredthroughacooperativeprocess.
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