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仪器网/ 行业应用/ 解决方案/ 结合TPEF二倍频和三倍频的飞秒振荡器显微术对秀丽杆线虫

结合TPEF二倍频和三倍频的飞秒振荡器显微术对秀丽杆线虫

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In this study, we use combined two-photon excitation
fluorescence (TPEF), second-harmonic generation (SHG) and
third-harmonic generation (THG) measurements to image
cellular structures of the nematode Caenorhabditis elegans, in
vivo.Toourknowledge, this is the first time thataTHGmodality
is employed to image live C. elegans specimens. Femtosecond
laser pulses (1028 nm) were utilized for excitation. Detailed
and specific structural and anatomical features can be
visualized, by recording THG signals. Thus, the combination
of three image-contrast modes (TPEF-SHG-THG) in a single
instrument has the potential to provide unique and
complementary information about the structure and function
of tissues and individual cells of live biological specimens. 飞秒激光器
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