pco.flim相机系统是采用Two-Tap CMOS成像芯片的荧光寿命成像相机。相机的像素和激发光间的同步使100皮秒至100微秒间的荧光衰减时间的分析成为可能。其多种触发选项使相机可以方便的集成于多种实际应用。
德国PCO公司首次发布了这款采用Two-Tap CMOS技术的荧光寿命成像系统Flim，应用范围涵盖分子特性分析和测试，FRET，离子浓度/PH值测量，生物组织学/病理学，压感材料等，可广泛应用于生命科学、物理科学，材料科学领域。
主要特点：
100 ps到100µs的寿命测量
调制频率从5千赫兹到40兆赫
500千赫兹到40兆赫外部调制信号
正弦/矩形的调制信号波形
像素分辨率1008 x 1008
频域FLIM
USB 3.0接口
量子效率39%
动态范围1000:1
读出噪音45 e- rms
90帧每秒 (2 tap读出)
可选曝光时间为10ns到10秒
无振动水冷
特殊的测量与分析软件
技术参数：
应用案例：
Figure 1: The left photo shows the fluorescence intensity of HEK-293 cells which expressed a CFP/DJ-1 protein as control of a FRET experiment. The middle image shows the phase angle derived distribution of fluorescence lifetimes in the range of 0 – 4 ns (imageJ LUT 16 colors, colorbar 0 – 4 ns) which has been masked by an intensity filter. The range of lifetimes around 2 ns was found in all of the 26 cells, which have been measured and showed about 10% FRET efficiency compared to the pure CFP expression. The right image shows the lifetime distribution image weighted by the fluorescence intensity image (the color images are false color coded using the same colorbar and LUT) without mask (courtesy of Prof. Dr. F.S. Wouters and Dr. G. Bunt, University Medicine Göttingen).
Figure 5:The left image shows the fluorescence intensity of a sliced daisy sample (20x air objective). The middle image shows the phase angle derived distribution of fluorescence lifetimes in the range of 0 - 4 ns (imageJ LUT 16 colors, colorbar 0-4 ns). The difference in fluorescence lifetimes clearly allows to differentiate the cells and the pollen grains. The right image shows the lifetime distribution image weighted by the fluorescence intensity image (the color images are false color coded, the modulation frequency was 30 MHz) (courtesy of Prof. Dr. F.S. Wouters and Dr. G. Bunt, University Medicine Göttingen).
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