肺炎链球菌脱氧核糖核酸液体室内质控品介绍:
产品中文名称:肺炎链球菌脱氧核糖核酸液体室内质控品
产品英文名称:Streptococcus pneumoniae DNA lab quality control
参考用途:本液体室内质控品为肺炎链球菌灭活菌悬液,通过荧光定量pcr结合数字PCR定值,可用于方法建立、工作标准赋值、方法确认与评价、产品质量控制等。
形态特性:/
培养基:特殊蛋白胨:23.0g,可溶性淀粉:1.0g,氯化钠:5.0g,琼脂:10.0g,脱纤维羊血:5%(50℃添加),pH:7.3±0.2(25℃)
培养条件:37℃,18-24h,5%CO2+95%空气
培养方法:从冰箱取出,完全融化后,涡旋振荡混匀,即可使用。注意:本质控品为灭活颗粒,需提取核酸后使用,提取或纯化试剂需自备,提取前避免反复冻融。最小取样量为200μl。
存储形式:-20℃
提供形式:冻存管;10^2~10^3copies/mL;10管/盒
备注:‖Antigenic Properties│Serotype 19F (Danish), type 19 (U.S.) (J H Jorgensen, personal communication)‖Antigenic Properties│Serotype 19F (Danish), type 19 (U.S.) (J H Jorgensen, personal communication)‖Cross References│Nucleotide (GenBank) Z74778 S.pneumoniae gene for dihydrofolate reductase.‖描述│Respiratory research
quality control strain Ref BD Phoenix Streptococcus SMIC/ID QC Set. BD Becton Dickinson; Phoenix Ref Sensititre™ H. influenzea/S. pneumoniae MIC Susceptibility. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems.
susceptibility disc testing Ref Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9. Ref Methods for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M42-A. Ref Methods for Antimicrobial Dilution and Disk susceptibility Testing of Infrequently Isolated or Fastidious Bacteria: Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M45-A. Ref Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M49-A.
susceptibility testing Ref Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8. Ref Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3. Ref Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007 Ref Evaluation of Lots of Dehydrated Mueller-Hinton Broth for Antimicrobial Susceptibility testing; Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M32-P. Ref Clinical laboratory testing and in vitro diagnostic test systems --- Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices --- Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. London, UK:British Standards Institution;British Standard BS EN ISO 20776-1:2006.;MIC values using HTM medium RefJorgensen JH, et al. Multicenter evaluation of the use of Haemophilus test medium for broth microdilution antimicrobial susceptibility testing of Streptococcus pneumoniae and development of quality control limits. J. Clin. Microbiol. 30: 961-966, 1992. PubMed: 1572984
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