pcDNA3.1/Zeo(+)

质粒类型：哺乳动物表达载体

启动子：CMV

表达水平：高

克隆方法：多克隆位点，限制性内切酶

载体大小：5015 bp (查看载体序列)

5' 测序引物:  pcDNA3.1-F:CTAGAGAACCCACTGCTTAC

3' 测序引物:  pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Zeocin 博来霉素

备注：

Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori.

This pcDNA™3.1/Zeo(+)vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Zeocin™ selectable marker and a forward-orientation multiple cloning site.

The pcDNA™3.1 Expression Vector Family

Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:

• Cytomegalovirus (CMV) enhancer-promoter for high-level expression

• Large multiple cloning site in either forward (+) or reverse (-) orientations

• Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability

• SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e.,COS-1 and COS-7)

• Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli