pcDNA3.1/V5-His B

质粒类型：哺乳动物表达载体

启动子：CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：5506bp

5' 测序引物及序列：T7 Fwd:5'd[TAATACGACTCACTATAGGG]3'

载体标签：His Tag (6x), V5 Epitope Tag

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Neo (新霉素)

All pcDNA™ vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.