-
-
Anti-Rab35-GTP Monoclonal Antibody/费斯德active Rab35
- 品牌:美国NewEast Biosciences
- 货号: 26922
- CAS编号:
- 产地:美国
- 供应商报价: 面议 (市场参考价:¥ 4800)
- 武汉费斯德生物科技有限公司 更新时间:2023-04-16 23:55:39
-
企业性质生产商
入驻年限第2年
营业执照已审核
- 同类产品活性单抗(23件)
-
为您推荐
- 详细介绍
货号: 26922 规格: 30 µL 基因符号: RAB35 描述: Anti-Rab35-GTP Monoclonal Antibody 背景介绍: The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions. 免疫原: Recombinant full length protein of active Rab35 经过测试的应用: IP, IHC, IF 推荐稀释比:
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250浓度: 1 mg/ml 宿主: mouse 形式: Liquid 克隆性: Monoclonal 亚型: IgG 纯度: Purified from ascites 防腐剂: No 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol 种属反应性: Anti-Rab35-GTP monoclonal antibody recognizes active Rab35 proteins of vertebrates. 储存条件: Store at -20°C. Avoid repeated freezing and thawing 免疫沉淀/蛋白质印迹:
Rab35 Activation Assay.Purified full-length Rab35 proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-Rab35-GTP monoclonal antibody (Cat. No. 26922). Immunoblot was with an anti-Rab35 polyclonal antibody (Cat. No. 21078).- 主要用途
- GTP酶是GTP/GDP结合蛋白。被细胞表面受体(GPCR)激活后, 它能调节下游效应器的功能。它可以结合GTP、水解GTP,因此在激活的GTP结合状态和非激活的GDP结合状态之间切换。它具有内在的GTP酶活性,可将GTP水解为GDP,从而使G蛋白失活。我们精心筛选的小鼠单克隆抗体能捕捉到与GTP结合的具有活性的GTP酶。它们已被用于通过免疫组织化学监测细胞和组织中 G 蛋白的活化和分布。