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武汉Gαi-GTP 小鼠单抗/费斯德现货
- 品牌：美国NewEast Biosciences
- 货号： 26901
- CAS编号：
- 产地：武汉
- 供应商报价： 面议 （市场参考价：¥ 4800）
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武汉费斯德生物科技有限公司
更新时间：2023-04-16 23:55:39 -
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- 详细介绍
货号: 26901 基因符号: Gnai 描述: Anti-Gαi-GTP Mouse Monoclonal Antibody 背景介绍: Heterotrimeric G proteins are critical cellular signal transducers. Gαi represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gα i proteins are being explored. 免疫原: Recombinant full length protein of active Gα i1 经过测试的应用: IP, IHC, IF 推荐稀释比:
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250浓度: 1 mg/ml 宿主: Mouse 形式: Liquid 克隆性: Monoclonal 亚型: IgG1 纯度: Purified from ascites 防腐剂: No 成分: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol 种属反应性: Anti-active Gα i antibody recognizes active Gα i1, Gα i2, and Gα i3 of vertebrates 储存条件: Store at -20°C. Avoid repeated freezing and thawing 免疫沉淀/蛋白质印迹: 
Gαi activation assay.
A. CHO cells were transfected with wild-type Gα i1 (lanes 1 and 2) or constitutively active Gα i1-Q204L (lane 3). Cell lysates were treated with GDP(lane 1) or GTPγS (lane 3). Lysates were then incubated with an anti-active Gα i monoclonal antibody (Cat. No.26901) (top panel). The precipitated active Gα i was immunoblotted with an anti-Gα i monoclonal antibody (Cat. No. 26003). The bottom panel shows the Western blot with anti-Gα i monoclonal antibody (Cat.No. 26003) of the cell lysates.
B. HEK293 cells stably expressing human m2 mAChR were treated with (lane 2) or without (lane 1)carbachol. Cell lysates were then incubated with an anti-active Gα i monoclonal antibody (Cat. No. 26901) (top panel).The precipitated active Gα i was immunoblotted with an anti-Gα i rabbit polyclonal antibody (Cat. No. 21006). The bottom panel shows the Western blot with anti-tubulin of the cell lysates.- 主要用途
- GTP酶是GTP/GDP结合蛋白。被细胞表面受体(GPCR)激活后, 它能调节下游效应器的功能。它可以结合GTP、水解GTP，因此在激活的GTP结合状态和非激活的GDP结合状态之间切换。它具有内在的GTP酶活性，可将GTP水解为GDP，从而使G蛋白失活。我们精心筛选的小鼠单克隆抗体能捕捉到与GTP结合的具有活性的GTP酶。它们已被用于通过免疫组织化学监测细胞和组织中 G 蛋白的活化和分布。
