载体质粒BioVector NTCCZG质粒载体菌种细胞基因保藏ZX

NTCC典型培养物保藏中心-BioVector质粒载体菌种细胞基因保藏中心，供应数十万种质粒、载体、菌种、基因、细胞、感受态细胞等产品。NTCC典型培养物保藏中心-BioVector质粒载体菌种细胞基因保藏中心资源种类齐全，涵盖标准菌种、质控菌株、食品检测菌种、药品检测菌种、环境监测菌种、NTCC/DSMZ/ATCC菌种细胞、克隆载体、真核表达载体、原核表达载体、动物细胞表达载体、慢病毒载体、腺病毒逆病毒载体、RNAi基因沉默基因敲除载体、抗体表达载体GS加压筛选悬浮细胞表达系统、信号通路报告载体、亚细胞定位载体、荧光蛋白载体、昆虫细胞/杆状病毒表达载体、植物表达载体RNAi干扰沉默载体、农杆菌菌株与感受态细胞、启动子载体、诱导调控载体、毕赤酵母/酿酒酵母表达载体、酵母表面展示系统、酵母单杂双杂三杂交系统、噬菌体展示抗体系统、枯草芽孢杆菌表达载体系统、乳酸菌表达系统、分支杆菌表达系统、假单胞菌表达载体基因敲除质粒、细胞自噬质粒载体LC3、细胞永生化质粒载体SV40/hTERT、iPS干细胞重编程载体系统、转座子载体、CRISPR/Cas9载体系统TALEN基因编辑系统、慢病毒荧光表达载体、果蝇/线虫表达载体、革兰氏阳性菌/金葡菌/链霉菌链球菌表达载体敲除载体、稀有基因合成克隆等几十种类型数十万个品种。并可提供质粒/菌种保藏鉴定、基因合成、基因克隆、质粒构建、蛋白原核、真核表达及纯化、病毒包装、基因沉默RNAi等实验技术服务。BioVector NTCC Inc.还可为您提供便捷一站式的产品进口服务，到货快捷，步骤简便。
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大肠杆菌DH5A supE44& lacU 196(U80lacZ&M 15) recA endA 本室保存      大肠杆菌DH5A supE44& lacU 196(U80lacZ&M 15) recA endA 本室保存        【Supplier来源】BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net  【Description描述】 大肠杆菌DH5A supE44& lacU 196(U80lacZ&M 15) recA endA 本室保存 BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心      
　成团泛菌308R 抗利福霉素自发突变株, 从番茄上分离 本室保存             
　pCPP9 粘粒载体 文献[5]             
　pExSec1 分泌表达载体, 与蛋白A 的 SZZ 融合表达, 受 T7启动子严谨调控,Km             
r 文献[6]             
　pTR102 含有RK2中3. 2kb 的稳定性片段, parCBA, parDE,Am             
r             
, Tc             
r 文献[4]             
　pZL1 parDE 克隆到pGEM 2T 载体上,Amp             
r 本文构建             
　pZL2 parDE 克隆到pExSec1的B amH I 位点上, 转录方向与 T7 启动子相同 本文构建             
　pZL3 parDE 克隆到pExSec1的B amH I 位点上, 转录方向与 T7 启动子相反 本文构建             
菌株或质粒              
Strains or plasmids              
描述              
Description              
参考文献或来源             
Reference or              
source              
Strains              
P. aeruginosa              
PAO1 Wild type; Apr             
              
PAO6421 The rsmY rsmZ double mutant; Apr             
 [18]              
Agrobacterium tumefaciens              
NTL4(pZLR4)              
A. tumefaciens NT1 derivative carrying a traG-lacZ reporter fusion, AHL              
biosensor; Gmr             
 [19]              
Escherichia coli DH5α supE44 lacU169 (80lacZ M15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 [20]              
Plasmids              
pPROBE-AT Promoter-probe vector; Cbr             
 [21]              
pAT-exoS pPROBE-AT containing a 510-bp fragment containing exoS promoter              
region; Cbr             
 [16]              
pPSV35 Shuttle vector, lacIq, and the lacUV5 promoter and MCS of pUC18; Gmr             
 [22]              
p35-exsA pPSV35 containing the exsA gene; Gmr             
 This study              
Strain/Plasmid Key Properties Reference           
E.Coli Strains             
S17-1 Smr Spr Tra+; pro thi hsdR recA; chromosomal integration of RP4-2-Tc::Mu-Kan::Tn7  λpir+ [21]           
JM109 recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F' [traD36, proAB+, lacIq, lacZΔM15 [20]           
DH5α F- , lacZΔαM15 endA1 recA1 hsdR17(rM- mK-) supE44 thi-1 gyrA96 Δ(lacZYA-argF)U169 [19]           
             
Plasmids              
pCVD442 Apr, R6K Ori and sacB counter selection; suicide vector [22]           
pCVD442-dapAX Used to delete the dapAX promoter and dapA open reading frame This Study           
pACYC177 Cloning vector; Apr Kanr, p15a origin of replication New England Biolabs           
pBR322 Cloning vector Apr Kanr, ColE1 origin of replication New England Biolabs           
pACYC-177dapA Apr, expresses dapA from the endogenous promoter; dapA inserted in place of Kanr gene. This Study           
pUC18R6KT Apr, R6K Ori ; mini-Tn7 transposition suicide vector, allows for single copy chromosomal insertion at attTn7 sites. Choi, 2005           
Mini-Tn7             
pTNS2 Apr, R6K Ori ;mini-Tn7-T helper plasmid, encodes TnsABC +D transposase complex to catalyze high frequency insertion Choi, 2005           
pDB1 pUC18R6KT; Apr, dapA inserted between att-Tn7 sites   This Study           
pDB2 pUC18R6KT; Apr, dapAX inserted between att-Tn7 sites This Study           
pDsRed-monomer Apr, lac driven DsRed expression Clontech           
ptdTomato Apr, lac driven Tomato expression Clontech           
pRsaI-2.1 Apr, cysZK driven DsRed expression This Study           
pNE160 Apr, cysZK driven Tomato expression This Study           
pLK52 Apr, pDB2 derivative with cysZK DsRed cassette from pRsaI-2.1 and dapAX between att-Tn7 sites This Study           
pNE168 Apr, pDB2 derivative with cysZK Tomato cassette from pNE160 and dapAX between att-Tn7 sites This Study           
pGEM-T easy Apr, blue/white screen cloning vector Promega           
Comamonas testosteroniI2 AniNC 3-CANCRifr, Hgr 4           
 Delftia acidovorans LME1 AniNC3-CANC Rifr This study           
 Delftia acidovorans B8c AniNC3-CAN This study           
 Delftia acidovoransCA28 AniNC 3-CANCRifr 34           
 Delftia acidovorans BN3.1 AniNC 3-CANCRifr 6           
 Ralstonia eutropha JMP228 Rifr 60           
 Ralstonia eutrophaJMP228gfp Rifr Kmr GFP This study           
 Escherichia coliS17-1 λpir  24           
 Escherichia coliS17-1 λpir(pUTgfp) Ampr Kmr GFP This study           
Plasmids             
 pNB2 (from strain I2) AniNCHgr This study           
 pNB1 (from strain LME1) AniN This study           
 pNB8c (from strain B8c) AniN This study           
 pC1-1 (from strain CA28) AniN 3-CAN This study           
 pC1-2 (from strain CA28) AniN This study           
 pC1-3 (from strain CA28) AniN 3-CAN This study           
 pB1 (from strain BN3.1)  This study           
 pUTgfp Ampr Kmr; expresses GFP 58           
 pTDN1-3112 pUC19 + HindIII (2.59 kb)-EcoRI (4.15 kb) fragment containing tdnQ ofP. putida UCC22; Ampr F. Fukumori           
 pBRH4 AmprIS1071 42           
Cumate Inducible Lentivectors Control technology for inducible cDNA or microRNA expression in mammalian cells            
Cumate solution (1000x), 500ul 500ul 115 EUR          
pCDH-EF1-CymR-T2A-Puro (plasmid) 10 ug 455 EUR          
pCDH-EF1-CymR-T2A-Puro (virus) >1 x 10^6 IFUs 485 EUR          
pCDH-EF1-CymR-T2A-RFP (plasmid) 10 ug 455 EUR          
pCDH-EF1-CymR-T2A-RFP (virus) >1 x 10^6 IFUs 485 EUR          
pCDH-Cuo-RFP-T2A-GFP (positive control plasmid) 10 ug 455 EUR          
pCDH-Cuo-RFP-T2A-GFP (positive control virus) >1 x 10^6 IFUs 485 EUR          
pCDH-CuO-MCS empty cloning lentivector 10 ug 455 EUR          
pCDH-CuO-MCS-EF1-GFP dual promoter lentivector 10 ug 515 EUR          
pCDH-CuO-MCS-EF1-RFP dual promoter lentivector 10 ug 515 EUR          
pCDH-CuO-MCS-T2A-GFP co-inducible T2A lentivector 10 ug 515 EUR          
pCDH-CuO-MCS-T2A-RFP co-inducible T2A lentivector 10 ug 515 EUR          
pCDH-CuO-MCS-IRES-GFP co-inducible IRES lentivector 10 ug 515 EUR          
pCDH-CuO-MCS-IRES-RFP co-inducible IRES lentivector 10 ug 515 EUR          
Cumate Starter Pack: Your choice of inducible vector below, + CymR-T2A-Puro (packaged virus, QM200VA-1) and Cumate solution (1000x, QM100A-1). Indicate your inducible lentivector catalog # when placing Order. 1 Kit 930 EUR          
Gene Analysis Complete Transcriptome RNA Amplification Kit and Interferon Response Detection Kit            
Full Spectrum Complete Transcriptome RNA Amplification Kit 10 reactions 460 EUR          
Full Spectrum MultiStart Primers for T7 IVT (10X) 40 reactions 245 EUR          
Differentiation Reporters Monitor stem cells during differentiaion            
Mouse Actc Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Mouse Actc Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Human MLC-2v Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Human MLC-2v Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Human Tnnt2 Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Human Tnnt2 Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Mouse Tnnt2 Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Mouse Tnnt2 Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Mouse SM22a Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Mouse SM22a Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Human GFAP Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Human GFAP Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Mouse GFAP Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Mouse GFAP Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Human CD11b Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Human CD11b Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Mouse EMR1 Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Mouse EMR1 Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Mouse IBA-1 Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Mouse IBA-1 Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Mouse Col2a1 Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Mouse Col2a1 Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Mouse CD44 Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Mouse CD44 Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Human BM88 Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Human BM88 Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Mouse Camk2a Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Mouse Camk2a Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Mouse GAD67 Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Mouse GAD67 Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Rat NSE Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Rat NSE Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Mouse Alpha-Tubulin Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Mouse Alpha-Tubulin Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Mouse MBP Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Mouse MBP Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Human Opsin Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Human Opsin Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Human Insulin Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Human Insulin Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Mouse Oct-4 Differentiation Reporter (pGreenZeo, plasmid) 10 ug 1190 EUR          
Mouse Oct-4 Differentiation Reporter (pGreenZeo, pre-packaged) >2 x 10^6 IFUs 585 EUR          
Human SPP-1 Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Human SPP-1 Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Human Nanog Differentiation Reporter (pGreenZeo, plasmid) 10 ug 1190 EUR          
Human Nanog Differentiation Reporter (pGreenZeo, pre-packaged) >2 x 10^6 IFUs 585 EUR          
Mouse Nanog Stem Cell Differentiation Reporter (pGreenZeo, plasmid) 10 ug 1190 EUR          
Mouse Nanog Stem Cell Differentiation Reporter (pGreenZeo, pre-packaged) >2 x 10^6 IFUs 585 EUR          
Human LCK Differentiation Reporter (pGreenZeo, Plasmid) 10 ug 1190 EUR          
Human LCK Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs 585 EUR          
Human Oct-4 Differentiation Reporter (pGreenZeo, plasmid) 10 ug 1190 EUR