NTCC典型培养物保藏ZX-BioVector质粒载体菌种细胞基因保藏ZX

​NTCC典型培养物保藏中心-BioVector质粒载体菌种细胞基因保藏中心，供应数十万种质粒、载体、菌种、基因、细胞、感受态细胞等产品。NTCC典型培养物保藏中心-BioVector质粒载体菌种细胞基因保藏中心资源种类齐全，涵盖标准菌种、质控菌株、食品检测菌种、药品检测菌种、环境监测菌种、NTCC/DSMZ/ATCC菌种细胞、克隆载体、真核表达载体、原核表达载体、动物细胞表达载体、慢病毒载体、腺病毒逆病毒载体、RNAi基因沉默基因敲除载体、抗体表达载体GS加压筛选悬浮细胞表达系统、信号通路报告载体、亚细胞定位载体、荧光蛋白载体、昆虫细胞/杆状病毒表达载体、植物表达载体RNAi干扰沉默载体、农杆菌菌株与感受态细胞、启动子载体、诱导调控载体、毕赤酵母/酿酒酵母表达载体、酵母表面展示系统、酵母单杂双杂三杂交系统、噬菌体展示抗体系统、枯草芽孢杆菌表达载体系统、乳酸菌表达系统、分支杆菌表达系统、假单胞菌表达载体基因敲除质粒、细胞自噬质粒载体LC3、细胞永生化质粒载体SV40/hTERT、iPS干细胞重编程载体系统、转座子载体、CRISPR/Cas9载体系统TALEN基因编辑系统、慢病毒荧光表达载体、果蝇/线虫表达载体、革兰氏阳性菌/金葡菌/链霉菌链球菌表达载体敲除载体、稀有基因合成克隆等几十种类型数十万个品种。并可提供质粒/菌种保藏鉴定、基因合成、基因克隆、质粒构建、蛋白原核、真核表达及纯化、病毒包装、基因沉默RNAi等实验技术服务。BioVector NTCC Inc.还可为您提供便捷一站式的产品进口服务，到货快捷，步骤简便。

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HCC2935 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2869 HCC2935 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2869<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HCC38 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2314 HCC38 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2314<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HCC38 BL cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2346 HCC38 BL cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2346<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HCC4006 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2871 HCC4006 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2871<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HCC70 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2315 HCC70 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2315<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HCC827 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2868 HCC827 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2868<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HCE-2 [50.B1] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11135 HCE-2 [50.B1] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11135<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HCN-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-10742 HCN-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-10742<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HCT 116 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-247 HCT 116 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-247<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HCT-15 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-225 HCT-15 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-225<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HCT-8 [HRT-18] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-244 HCT-8 [HRT-18] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-244<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

He We cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1338 He We cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1338<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HEC-1-A cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-112 HEC-1-A cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-112<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HEC-1-B cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-113 HEC-1-B cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-113<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HEK 293 STF cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3249 HEK 293 STF cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3249<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HEK001 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2404 HEK001 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2404<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HEK-293.2sus cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1573.3 HEK-293.2sus cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1573.3<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HEK293S GnTI- cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3022 HEK293S GnTI- cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3022<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HEL 299 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-137 HEL 299 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-137<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HEL 92.1.7 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 TIB-180 HEL 92.1.7 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 TIB-180<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HeLa cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-2 HeLa cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-2<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HeLa [Chang Liver] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-13 HeLa [Chang Liver] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-13<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HeLa 229 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-2.1 HeLa 229 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-2.1<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HeLa NR1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-13011 HeLa NR1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-13011<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HeLa S3 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-2.2 HeLa S3 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-2.2<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HeLaRC32 [HeRC32] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2972 HeLaRC32 [HeRC32] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2972<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hep 3B2.1-7 [Hep 3B, Hep-3B, Hep3B] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心HB-8064 Hep 3B2.1-7 [Hep 3B, Hep-3B, Hep3B] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心HB-8064<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hep G2 [HEPG2] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HB-8065 Hep G2 [HEPG2] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HB-8065<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HEP G2/2.2.1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11997 HEP G2/2.2.1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11997<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HEp-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-23 HEp-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-23<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HEPM cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1486 HEPM cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1486<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Het-1A cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2692 Het-1A cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2692<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HEY-T30 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3252 HEY-T30 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3252<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HF 282.Sp cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7701 HF 282.Sp cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7701<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HFL1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-153 HFL1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-153<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

hFOB 1.19 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11372 hFOB 1.19 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11372<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HG-261 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-122 HG-261 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-122<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HGF-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2014 HGF-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2014<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HH cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2105 HH cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2105<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HIEC-6 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3266 HIEC-6 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3266<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HK-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2190 HK-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2190<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HL-60 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-240 HL-60 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-240<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HL-60/MX1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2258 HL-60/MX1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2258<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HL-60/MX2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2257 HL-60/MX2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2257<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HL-60/S4 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3306 HL-60/S4 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3306<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HLF-a cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-199 HLF-a cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-199<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HM2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HB-8587 HM2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HB-8587<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HMC3 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3304 HMC3 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3304<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HMCB [ Human Melanoma Cell Bowes] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心CRL-9607 HMCB [ Human Melanoma Cell Bowes] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心CRL-9607<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HMEC-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3243 HMEC-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3243<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HMy2.CIR [C1R, HMy2.C1R] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1993 HMy2.CIR [C1R, HMy2.C1R] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1993<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HOS cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1543 HOS cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1543<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HP [HT1080 poly] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-12012 HP [HT1080 poly] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-12012<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HPAC cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2119 HPAC cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2119<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HPAF-II cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1997 HPAF-II cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1997<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HRT-18G cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11663 HRT-18G cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11663<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 1.Int cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7820 Hs 1.Int cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7820<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 1.Lu cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7000 Hs 1.Lu cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7000<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 1.Sk/Mu cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7001 Hs 1.Sk/Mu cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7001<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 1.Tes cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7002 Hs 1.Tes cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7002<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 127.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7081 Hs 127.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7081<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 132.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7085 Hs 132.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7085<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 14.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7823 Hs 14.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7823<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 142.Sp cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7090 Hs 142.Sp cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7090<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 143.We cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7092 Hs 143.We cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7092<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 144.We cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7093 Hs 144.We cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7093<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 181.Tes cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7131 Hs 181.Tes cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7131<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 184.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7134 Hs 184.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7134<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 188.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7140 Hs 188.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7140<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 190.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7145 Hs 190.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7145<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 198.Ton cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7156 Hs 198.Ton cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7156<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 202.Th cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7163 Hs 202.Th cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7163<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 209.Sp cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7171 Hs 209.Sp cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7171<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 225.Th cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7191 Hs 225.Th cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7191<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 228.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7193 Hs 228.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7193<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 232.Th cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7197 Hs 232.Th cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7197<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 235.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7201 Hs 235.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7201<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 255.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7213 Hs 255.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7213<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 257.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7214 Hs 257.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7214<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 281.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7227 Hs 281.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7227<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 294T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-140 Hs 294T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-140<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 295.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7232 Hs 295.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7232<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 3.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7005 Hs 3.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7005<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 313.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7235 Hs 313.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7235<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 319.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7236 Hs 319.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7236<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 324.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7239 Hs 324.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7239<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 343.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7245 Hs 343.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7245<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 344.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7246 Hs 344.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7246<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 350.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7248 Hs 350.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7248<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 357.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7252 Hs 357.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7252<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 362.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7253 Hs 362.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7253<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 38.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7826 Hs 38.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7826<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 387.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7263 Hs 387.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7263<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 39.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7023 Hs 39.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7023<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 414.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7287 Hs 414.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7287<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 445 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-146 Hs 445 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-146<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 454.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7802 Hs 454.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7802<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 456.Bt cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7805 Hs 456.Bt cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7805<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 456.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7804 Hs 456.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7804<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 46.Fs cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7026 Hs 46.Fs cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7026<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 5.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7822 Hs 5.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7822<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 505.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7306 Hs 505.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7306<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 52.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7031 Hs 52.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7031<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 53.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7033 Hs 53.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7033<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 564(E.Mg cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7329 Hs 564(E.Mg cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7329<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 566(B.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7336 Hs 566(B.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7336<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 57.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7037 Hs 57.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7037<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 573.Lu cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7344 Hs 573.Lu cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7344<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 573.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7343 Hs 573.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7343<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 574.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7345 Hs 574.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7345<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 578Bst cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-125 Hs 578Bst cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-125<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 578T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-126 Hs 578T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-126<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 579.Mg cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7347_FL Hs 579.Mg cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7347_FL<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 588.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7850 Hs 588.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7850<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 590.We cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7353 Hs 590.We cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7353<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 600.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7360 Hs 600.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7360<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 602 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-142 Hs 602 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-142<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 604.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7362 Hs 604.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7362<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 605.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7364 Hs 605.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7364<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【

Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 605.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7365 Hs 605.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7365<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 606.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7368 Hs 606.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7368<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 611.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7373 Hs 611.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7373<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 616.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7378 Hs 616.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7378<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 617.Mg cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7379_FL Hs 617.Mg cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7379_FL<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 618.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7380_FL Hs 618.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7380_FL<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 63.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7043 Hs 63.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7043<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 67 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-163 Hs 67 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-163<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 675.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7400 Hs 675.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7400<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 677.Tg cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7408 Hs 677.Tg cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7408<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 680.Rec cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7418 Hs 680.Rec cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7418<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 680.Tg cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7421 Hs 680.Tg cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7421<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 680.Tr cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7422 Hs 680.Tr cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7422<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 683 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-138 Hs 683 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-138<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 688(A.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7425 Hs 688(A.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7425<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 688(B.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7426 Hs 688(B.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7426<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 692(A.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7428 Hs 692(A.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7428<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 695T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-137 Hs 695T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-137<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 696 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-151 Hs 696 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-151<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 698.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7435 Hs 698.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7435<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 700T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-147 Hs 700T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-147<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 704.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7443 Hs 704.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7443<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 704.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7444 Hs 704.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7444<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 706.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7447 Hs 706.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7447<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 707(A.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7448 Hs 707(A.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7448<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 707(B.Ep cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7449 Hs 707(B.Ep cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7449<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 726.Pl cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7460 Hs 726.Pl cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7460<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 729 [Hs 729T] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-153 Hs 729 [Hs 729T] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-153<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 730.Pl cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7464 Hs 730.Pl cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7464<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 733.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7469 Hs 733.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7469<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 737.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7473 Hs 737.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7473<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 738.St/Int cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7869 Hs 738.St/Int cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7869<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 740.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7478 Hs 740.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7478<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 741.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7480 Hs 741.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7480<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 742.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7481 Hs 742.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7481<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 742.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7482 Hs 742.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7482<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 746T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-135 Hs 746T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-135<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 748.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7486 Hs 748.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7486<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 755(B.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7489_FL Hs 755(B.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7489_FL<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 766T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-134 Hs 766T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-134<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 777.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7507 Hs 777.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7507<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 781.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7511 Hs 781.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7511<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 789.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7518 Hs 789.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7518<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 789.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7886 Hs 789.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7886<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 792(C.M cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7522 Hs 792(C.M cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7522<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 793.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7523 Hs 793.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7523<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 795.Pl cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7526 Hs 795.Pl cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7526<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 798.Pl cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7529 Hs 798.Pl cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7529<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 799.Pl cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7530 Hs 799.Pl cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7530<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 804.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7535 Hs 804.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7535<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 819.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7891 Hs 819.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7891<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 821.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7554 Hs 821.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7554<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 822.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7556 Hs 822.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7556<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 835.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7569 Hs 835.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7569<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 839.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7572 Hs 839.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7572<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 840.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7573 Hs 840.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7573<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 841.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7574_FL Hs 841.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7574_FL<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 849.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7583_FL Hs 849.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7583_FL<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 851.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7584_FL Hs 851.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7584_FL<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 852.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7585 Hs 852.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7585<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 855.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7592 Hs 855.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7592<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 856.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7593 Hs 856.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7593<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 860.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7595 Hs 860.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7595<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 861.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7596 Hs 861.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7596<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 863.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7598 Hs 863.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7598<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 865.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7601 Hs 865.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7601<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 867.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7603 Hs 867.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7603<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 870.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7606 Hs 870.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7606<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 875.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7612 Hs 875.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7612<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 88.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7060 Hs 88.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7060<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 888.Lu cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7624 Hs 888.Lu cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7624<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 888.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7623 Hs 888.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7623<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 888.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7622 Hs 888.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7622<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 889.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7625_FL Hs 889.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7625_FL<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 890.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7627_FL Hs 890.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7627_FL<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 890.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7628 Hs 890.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7628<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 891.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7629 Hs 891.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7629<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 894(A.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7631_FL Hs 894(A.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7631_FL<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 895.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7636 Hs 895.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7636<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 895.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7637 Hs 895.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7637<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 898.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7641_FL Hs 898.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7641_FL<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 899(C.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7644_FL Hs 899(C.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7644_FL<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 906(A.M cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7653 Hs 906(A.M cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7653<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 906(B.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7654 Hs 906(B.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7654<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 910.Thm cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7660_FL Hs 910.Thm cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7660_FL<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 913(F.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7668 Hs 913(F.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7668<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 913T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-152 Hs 913T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-152<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 917.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7669 Hs 917.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7669<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 919.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7671 Hs 919.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7671<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 919.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7672 Hs 919.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7672<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 925.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7676 Hs 925.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7676<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 925.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7677 Hs 925.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7677<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 926.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7678 Hs 926.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7678<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 929.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7681 Hs 929.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7681<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 93.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7062 Hs 93.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7062<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 934.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7684 Hs 934.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7684<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 936.T(C1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7687 Hs 936.T(C1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7687<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 939.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7690 Hs 939.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7690<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 94.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7064 Hs 94.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7064<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 940.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7691 Hs 940.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7691<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 944.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7693 Hs 944.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7693<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs 97.Fs cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7065 Hs 97.Fs cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7065<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs27 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1634 Hs27 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1634<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HS-27A cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2496 HS-27A cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2496<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HS-5 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11882 HS-5 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11882<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs68 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1635 Hs68 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1635<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Hs888Lu cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-211 Hs888Lu cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-211<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HS-Sultan cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1484 HS-Sultan cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1484<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HT cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2260 HT cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2260<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HT 297.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7782 HT 297.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7782<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HT-1080 [HT1080] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-121 HT-1080 [HT1080] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-121<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HT-1197 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1473 HT-1197 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1473<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HT-1376 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1472 HT-1376 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1472<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HT-144 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-63 HT-144 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-63<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HT-29 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-38 HT-29 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-38<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HT-3 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-32 HT-3 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-32<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HTR-8/SVneo cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3271 HTR-8/SVneo cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3271<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HTZ17XP cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1361 HTZ17XP cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1361<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HuLa-PC cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3342 HuLa-PC cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3342<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HULEC-5a cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3244 HULEC-5a cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3244<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HuNS1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-8644 HuNS1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-8644<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HuT 102 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 TIB-162 HuT 102 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 TIB-162<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HuT 78 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 TIB-161 HuT 78 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 TIB-161<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

HuTu 80 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-40 HuTu 80 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-40<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agi