NTCC典型培养物保藏ZX-BioVector质粒载体菌种细胞基因保藏ZX

NTCC典型培养物保藏中心-BioVector质粒载体菌种细胞基因保藏中心，供应数十万种质粒、载体、菌种、基因、细胞、感受态细胞等产品。NTCC典型培养物保藏中心-BioVector质粒载体菌种细胞基因保藏中心资源种类齐全，涵盖标准菌种、质控菌株、食品检测菌种、药品检测菌种、环境监测菌种、NTCC/DSMZ/ATCC菌种细胞、克隆载体、真核表达载体、原核表达载体、动物细胞表达载体、慢病毒载体、腺病毒逆病毒载体、RNAi基因沉默基因敲除载体、抗体表达载体GS加压筛选悬浮细胞表达系统、信号通路报告载体、亚细胞定位载体、荧光蛋白载体、昆虫细胞/杆状病毒表达载体、植物表达载体RNAi干扰沉默载体、农杆菌菌株与感受态细胞、启动子载体、诱导调控载体、毕赤酵母/酿酒酵母表达载体、酵母表面展示系统、酵母单杂双杂三杂交系统、噬菌体展示抗体系统、枯草芽孢杆菌表达载体系统、乳酸菌表达系统、分支杆菌表达系统、假单胞菌表达载体基因敲除质粒、细胞自噬质粒载体LC3、细胞永生化质粒载体SV40/hTERT、iPS干细胞重编程载体系统、转座子载体、CRISPR/Cas9载体系统TALEN基因编辑系统、慢病毒荧光表达载体、果蝇/线虫表达载体、革兰氏阳性菌/金葡菌/链霉菌链球菌表达载体敲除载体、稀有基因合成克隆等几十种类型数十万个品种。并可提供质粒/菌种保藏鉴定、基因合成、基因克隆、质粒构建、蛋白原核、真核表达及纯化、病毒包装、基因沉默RNAi等实验技术服务。BioVector NTCC Inc.还可为您提供便捷一站式的产品进口服务，到货快捷，步骤简便。

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Tissue: Clivus Tissue: Clivus<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Colon Tissue: Colon<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Colon; Derived From Metastatic Site: Ascites Tissue: Colon; Derived From Metastatic Site: Ascites<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Colon; Derived From Metastatic Site: Left Supraclavicular Region Tissue: Colon; Derived From Metastatic Site: Left Supraclavicular Region<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Colon; Derived From Metastatic Site: Lung Tissue: Colon; Derived From Metastatic Site: Lung<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Colon; Derived From Metastatic Site: Lymph Node Tissue: Colon; Derived From Metastatic Site: Lymph Node<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Colon; Derived From Metastatic Site: Peritoneum Tissue: Colon; Derived From Metastatic Site: Peritoneum<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Colon; Derived From Metastatic Site: Connective Tissue Tissue: Colon; Derived From Metastatic Site: Connective Tissue<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Connective And Soft Tissue Tissue: Connective And Soft Tissue<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Connective Tissue Tissue: Connective Tissue<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Cord Blood Tissue: Cord Blood<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Derived From Metastatic Site: Bone (Sacrum) Tissue: Derived From Metastatic Site: Bone (Sacrum)<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Derived From Metastatic Site: Bone Marrow Tissue: Derived From Metastatic Site: Bone Marrow<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Derived From Metastatic Site: Lung Tissue: Derived From Metastatic Site: Lung<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Derived From Metastatic Site: Lung Or Bronchus Tissue: Derived From Metastatic Site: Lung Or Bronchus<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Derived From Metastatic Site: Lymph Node Tissue: Derived From Metastatic Site: Lymph Node<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Derived From Metastatic Site: Pelvis Tissue: Derived From Metastatic Site: Pelvis<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Derived From Metastatic Site: Pleural Effusion Tissue: Derived From Metastatic Site: Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Derived From Metastatic Site: Tongue Tissue: Derived From Metastatic Site: Tongue<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Derived From Metastatic Site:&Nbsp;Pleural Effusion Tissue: Derived From Metastatic Site:&Nbsp;Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Derived From: Hypopharynx Tissue: Derived From: Hypopharynx<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Dermal Endothelium Tissue: Dermal Endothelium<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Ectocervix; Cervix Tissue: Ectocervix; Cervix<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Embryo Tissue: Embryo<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Embryo, Placenta Tissue: Embryo, Placenta<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Embryonic Kidney Tissue: Embryonic Kidney<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Endocervix; Cervix Tissue: Endocervix; Cervix<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Esophagus Tissue: Esophagus<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Eye, Cornea Tissue: Eye, Cornea<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Eye, Retina Tissue: Eye, Retina<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Eye; Lens Tissue: Eye; Lens<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Eye; Retinal Pigmented Epithelium; Retina Tissue: Eye; Retinal Pigmented Epithelium; Retina<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Fetus, Whole Tissue: Fetus, Whole<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Foreskin Tissue: Foreskin<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Gingival Biopsy Tissue: Gingival Biopsy<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Hela Contaminant Tissue: Hela Contaminant<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Heteromyeloma Tissue: Heteromyeloma<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Histiocytic Lymphoma Tissue: Histiocytic Lymphoma<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Intestine Tissue: Intestine<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Kidney Tissue: Kidney<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Kidney, Cortex, Proximal Tubule, Cyst Tissue: Kidney, Cortex, Proximal Tubule, Cyst<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Kidney, Cortex/Proximal Tubule Tissue: Kidney, Cortex/Proximal Tubule<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Kidney; Cyst From A Distal And Proximal Cortical Tubule Tissue: Kidney; Cyst From A Distal And Proximal Cortical Tubule<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Kidney; Derived From Metastatic Site: Pleural Effusion Tissue: Kidney; Derived From Metastatic Site: Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Kidney; Derived From Metastatic Site: Skin Tissue: Kidney; Derived From Metastatic Site: Skin<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Kidney; Derived From Metastatic Site: Pleural Effusion Tissue: Kidney; Derived From Metastatic Site: Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Left Bicep, Subcutaneous Tissue: Left Bicep, Subcutaneous<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Liver Tissue: Liver<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Liver / Ascites Tissue: Liver / Ascites<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Liver/Left Lobe Tissue: Liver/Left Lobe<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Liver; Metastasis From Eye Melanoma Tissue: Liver; Metastasis From Eye Melanoma<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung Tissue: Lung<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung Adenocarcinoma; Derived From Metastatic Site: Pleural Effusion Tissue: Lung Adenocarcinoma; Derived From Metastatic Site: Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung, Bronchus Tissue: Lung, Bronchus<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung,&Nbsp;Bronchus Tissue: Lung,&Nbsp;Bronchus<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung/Bronchiole; Derived From Metastatic Site: Alveolus Tissue: Lung/Bronchiole; Derived From Metastatic Site: Alveolus<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung/Bronchus Tissue: Lung/Bronchus<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung/Pleural Fluid Tissue: Lung/Pleural Fluid<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung: Pleural Effusion Tissue: Lung: Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung; Bronchus Tissue: Lung; Bronchus<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung; Derived From Metastatic Site: Bone Marrow Tissue: Lung; Derived From Metastatic Site: Bone Marrow<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung; Derived From Metastatic Site: Cervix Tissue: Lung; Derived From Metastatic Site: Cervix<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung; Derived From Metastatic Site: Liver Tissue: Lung; Derived From Metastatic Site: Liver<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung; Derived From Metastatic Site: Lymph Node Tissue: Lung; Derived From Metastatic Site: Lymph Node<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung; Derived From Metastatic Site: Pleura Tissue: Lung; Derived From Metastatic Site: Pleura<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung; Derived From Metastatic Site: Pleural Effusion Tissue: Lung; Derived From Metastatic Site: Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung; Derived From Metastatic Site: Soft Tissue Tissue: Lung; Derived From Metastatic Site: Soft Tissue<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lung; Derived From Metatstatic Site: Bone Marrow Tissue: Lung; Derived From Metatstatic Site: Bone Marrow<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lymph Node Tissue: Lymph Node<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lymph Node; Derived From Metastatic Site: Ovary Tissue: Lymph Node; Derived From Metastatic Site: Ovary<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lymph Node; Derived From Metastatic Site: Peritoneal Effusion Tissue: Lymph Node; Derived From Metastatic Site: Peritoneal Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lymph Node; Derived From Metastatic Site: Skin Of Trunk Tissue: Lymph Node; Derived From Metastatic Site: Skin Of Trunk<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lymph Node; Derived From Metastatic Site: Peritoneal Effusion Tissue: Lymph Node; Derived From Metastatic Site: Peritoneal Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Lymphoblast Tissue: Lymphoblast<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Malignant Melanoma; Skin; Derived From Metastatic Site: Lymphatic System Tissue: Malignant Melanoma; Skin; Derived From Metastatic Site: Lymphatic System<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Malignant Melanoma; Derived From Metastatic Site: Subcutaneous Tissue Tissue: Malignant Melanoma; Derived From Metastatic Site: Subcutaneous Tissue<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Malignant Melanoma;&Nbsp;Derived From Metastatic Site: Lung Tissue: Malignant Melanoma;&Nbsp;Derived From Metastatic Site: Lung<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland Tissue: Mammary Gland<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland /Breast Tissue: Mammary Gland /Breast<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland, Breast Tissue: Mammary Gland, Breast<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland, Breast; Derived From Metastatic Site: Pleural Effusion Tissue: Mammary Gland, Breast; Derived From Metastatic Site: Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland/Breast Tissue: Mammary Gland/Breast<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland/Breast Duct; Derived From Metastatic Site: Axillary Lymph Node Tissue: Mammary Gland/Breast Duct; Derived From Metastatic Site: Axillary Lymph Node<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland/Breast: Epithelium Tissue: Mammary Gland/Breast: Epithelium<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland/Breast; Derived From Metastatic Site: Adenocarcinoma And Pleural Effusion Tissue: Mammary Gland/Breast; Derived From Metastatic Site: Adenocarcinoma And Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland/Breast; Derived From Metastatic Site: Ascites Tissue: Mammary Gland/Breast; Derived From Metastatic Site: Ascites<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland/Breast; Derived From Metastatic Site: Pleural Effusion Tissue: Mammary Gland/Breast; Derived From Metastatic Site: Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland; Breast Tissue: Mammary Gland; Breast<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland; Breast/Duct Tissue: Mammary Gland; Breast/Duct<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland; Breast/Duct; Derived From Metastatic Site: Ascites Tissue: Mammary Gland; Breast/Duct; Derived From Metastatic Site: Ascites<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland; Breast/Duct; Derived From Metastatic Site: Pleural Effusion Tissue: Mammary Gland; Breast/Duct; Derived From Metastatic Site: Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland; Breast/Epithelium Tissue: Mammary Gland; Breast/Epithelium<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland; Breast; Derived From Metastatic Site: Pleural Effusion Tissue: Mammary Gland; Breast; Derived From Metastatic Site: Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland; Breast; Derived From Metastatic Site: Pleural Fluid Tissue: Mammary Gland; Breast; Derived From Metastatic Site: Pleural Fluid<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland; Breast; Derived From Metastatic Site: Skin Carcinoma Tissue: Mammary Gland; Breast; Derived From Metastatic Site: Skin Carcinoma<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland; Breast; Duct Tissue: Mammary Gland; Breast; Duct<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mammary Gland; Breast; Pleural Effusion Tissue: Mammary Gland; Breast; Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Melanoma , Skin Tissue: Melanoma , Skin<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Melanoma Metastasis Tissue: Melanoma Metastasis<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Melanoma, Brain Metastasis Tissue: Melanoma, Brain Metastasis<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Melanoma, Lymph Node Metastasis Tissue: Melanoma, Lymph Node Metastasis<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Melanoma, Pelvic Soft Tissue Metastasis Tissue: Melanoma, Pelvic Soft Tissue Metastasis<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mesencephalon Tissue: Mesencephalon<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mesothelium Tissue: Mesothelium<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Metastatic Axillary Lymph Node. Primary Site Unknown Tissue: Metastatic Axillary Lymph Node. Primary Site Unknown<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Metastatic Site: Left Thigh Subcutaneous; Primary Site: Calf Tissue: Metastatic Site: Left Thigh Subcutaneous; Primary Site: Calf<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Metastatic: Left Lower Lung; Primary Site: Scalp Tissue: Metastatic: Left Lower Lung; Primary Site: Scalp<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Metastatic: Primary Site: Unknown; Metastatic Site: Right Inguinal Lymph Node Tissue: Metastatic: Primary Site: Unknown; Metastatic Site: Right Inguinal Lymph Node<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Metastatic: Primary Site: Unknown; Metastatic Site: Right Occipital Brain Tissue: Metastatic: Primary Site: Unknown; Metastatic Site: Right Occipital Brain<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mixed; Skin; Muscle Tissue: Mixed; Skin; Muscle<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Mixed; Spleen; Thymus Tissue: Mixed; Spleen; Thymus<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Muscle Tissue: Muscle<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Muscle; Connective And Soft Tissue Tissue: Muscle; Connective And Soft Tissue<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Muscle; Derived From Metastatic Site: Bone Marrow Tissue: Muscle; Derived From Metastatic Site: Bone Marrow<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Nasal Septum; Derived From Metastatic Site: Pleural Effusion Tissue: Nasal Septum; Derived From Metastatic Site: Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Ovary Tissue: Ovary<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Ovary: Ascites Tissue: Ovary: Ascites<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Ovary; Derived From Metastatic Site: Ascites Tissue: Ovary; Derived From Metastatic Site: Ascites<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Ovary; Derived From Metastatic Site: Subserosa Of Fallopian Tube Tissue: Ovary; Derived From Metastatic Site: Subserosa Of Fallopian Tube<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Palatal Mesenchyme Tissue: Palatal Mesenchyme<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Pancreas Tissue: Pancreas<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Pancreas/Duct Tissue: Pancreas/Duct<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Pancreas; Derived From Metastatic Site: Ascites Tissue: Pancreas; Derived From Metastatic Site: Ascites<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Pancreas; Derived From Metastatic Site: Liver Tissue: Pancreas; Derived From Metastatic Site: Liver<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Pancreas; Derived From Metastatic Site: Lymph Node Tissue: Pancreas; Derived From Metastatic Site: Lymph Node<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Pancreas; Derived From Metastatic Site: Peritoneal Mass Tissue: Pancreas; Derived From Metastatic Site: Peritoneal Mass<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Pancreas; Derived From Metastatic: Liver Tissue: Pancreas; Derived From Metastatic: Liver<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Pancreas; Derived From Metastatic Site:&Nbsp;Spleen Tissue: Pancreas; Derived From Metastatic Site:&Nbsp;Spleen<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Parotid Salivary Gland Tissue: Parotid Salivary Gland<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Peripheral Blood Tissue: Peripheral Blood<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Peripheral Blood, Blood Tissue: Peripheral Blood, Blood<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Peritoneal Effusion Tissue: Peritoneal Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Peritonial Effusion; Derived From Metastatic Site: Malignant Cerebrospinal Fluid Tissue: Peritonial Effusion; Derived From Metastatic Site: Malignant Cerebrospinal Fluid<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Pharynx Tissue: Pharynx<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Pharynx: Derived From Metastatic Site: Pleural Effusion Tissue: Pharynx: Derived From Metastatic Site: Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Placenta Tissue: Placenta<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Pleura, Pleural Effusion Tissue: Pleura, Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Pleura/Pleural Effusion, Lymphocyte, Myeloid Tissue: Pleura/Pleural Effusion, Lymphocyte, Myeloid<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Pleural Effusion Tissue: Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Posterior Commissure Of The Larynx Tissue: Posterior Commissure Of The Larynx<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Previously Described As: Mammary Gland/Breast; Derived From Metastatic Site: Pleural Effusion Tissue: Previously Described As: Mammary Gland/Breast; Derived From Metastatic Site: Pleural Effusion<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Primary Site: Clivus Tissue: Primary Site: Clivus<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Primary Site: Unknown; Metastatic: Right Inguinal Lymph Node Tissue: Primary Site: Unknown; Metastatic: Right Inguinal Lymph Node<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Primary Site: Thyroid; Metastatic Site: Neck Lymph Node Tissue: Primary Site: Thyroid; Metastatic Site: Neck Lymph Node<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Primary: Foot; Metastatic Site: Right Occipital Brain Tissue: Primary: Foot; Metastatic Site: Right Occipital Brain<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Primary: Right Forearm Tissue: Primary: Right Forearm<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Primary: Unknown; Metastatic Site: Left Axillary Lymph Node Tissue: Primary: Unknown; Metastatic Site: Left Axillary Lymph Node<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Primary: Unknown; Metastatic Site: Spleen Tissue: Primary: Unknown; Metastatic Site: Spleen<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Prostate Tissue: Prostate<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Prostate Peripheral Zone Tissue: Prostate Peripheral Zone<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Prostate, Normal, Peripheral Zone Tissue: Prostate, Normal, Peripheral Zone<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Prostate/Stroma Tissue: Prostate/Stroma<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Prostate; Derived From Metastatic Site: Bone Tissue: Prostate; Derived From Metastatic Site: Bone<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Prostate; Derived From Metastatic Site: Brain Tissue: Prostate; Derived From Metastatic Site: Brain<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Prostate; Derived From Metastatic Site: Left Supraclavicular Lymph Node Tissue: Prostate; Derived From Metastatic Site: Left Supraclavicular Lymph Node<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Prostate; Derived From Metastatic Site: Vertebral Metastasis Tissue: Prostate; Derived From Metastatic Site: Vertebral Metastasis<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Prostate; Derived From The Metastatic Site: Lymph Node Tissue: Prostate; Derived From The Metastatic Site: Lymph Node<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Prostate; Epithelium Tissue: Prostate; Epithelium<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Rectum Tissue: Rectum<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Retroperitoneal Embryonal Tumor Tissue: Retroperitoneal Embryonal Tumor<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Retroperitoneum Tissue: Retroperitoneum<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Retroperitoneum; Recurring Tissue: Retroperitoneum; Recurring<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Sacral Bone Tissue: Sacral Bone<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Skin Tissue: Skin<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Skin From Behind The Ear Tissue: Skin From Behind The Ear<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Skin, Derived From Metastatic Site: Lymph Node Tissue: Skin, Derived From Metastatic Site: Lymph Node<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Skin, Foreskin Tissue: Skin, Foreskin<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Skin/Epidermis Tissue: Skin/Epidermis<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Skin: Derived From Metastatic Axillary Node. Tissue: Skin: Derived From Metastatic Axillary Node.<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Skin:Derived From Metastasis On Skin Of Thigh. Tissue: Skin:Derived From Metastasis On Skin Of Thigh.<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Skin; Derived From Metastatic Site: Lymph Node Tissue: Skin; Derived From Metastatic Site: Lymph Node<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Skin; Epidermis Tissue: Skin; Epidermis<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Skin; Foreskin Tissue: Skin; Foreskin<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Small Intestine Tissue: Small Intestine<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Soft Tissue Tissue: Soft Tissue<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Somatic Cell Hybrid Tissue: Somatic Cell Hybrid<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Spleen Tissue: Spleen<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Stomach Tissue: Stomach<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Stomach; Derived From Metastatic Site: Ascites Tissue: Stomach; Derived From Metastatic Site: Ascites<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Stomach; Derived From Metastatic Site: Left Leg Tissue: Stomach; Derived From Metastatic Site: Left Leg<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Stomach; Derived From Metastatic Site: Liver Tissue: Stomach; Derived From Metastatic Site: Liver<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Submaxillary Salivary Gland Tissue: Submaxillary Salivary Gland<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Synovium Tissue: Synovium<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Testis Tissue: Testis<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Testis; Derived From Metastatic Site: Lung Tissue: Testis; Derived From Metastatic Site: Lung<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Testis; Derived From Metastatic Site: Lymph Node Tissue: Testis; Derived From Metastatic Site: Lymph Node<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Thymus Tissue: Thymus<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Thyroid Tissue: Thyroid<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Thyroid Tumor Tissue: Thyroid Tumor<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Thyroid/Medulla Tissue: Thyroid/Medulla<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Tongue Tissue: Tongue<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Tonsil Tissue: Tonsil<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Trachea Tissue: Trachea<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Umbilical Vein/Vascular Endothelium Tissue: Umbilical Vein/Vascular Endothelium<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Upper Maxilla Tissue: Upper Maxilla<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Ureter Tissue: Ureter<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Ureter, Immortalized Epithelial Sv40-Transformed Tissue: Ureter, Immortalized Epithelial Sv40-Transformed<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Ureter, Uroepithelium Tissue: Ureter, Uroepithelium<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Urinary Bladder Tissue: Urinary Bladder<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Uterine Myometrium Smooth Muscle Tissue: Uterine Myometrium Smooth Muscle<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Uterus Tissue: Uterus<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Uterus; Endometrium Tissue: Uterus; Endometrium<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Uveal, Eye Tissue: Uveal, Eye<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Vagina, Mucosa Tissue: Vagina, Mucosa<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Vulva Tissue: Vulva<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tissue: Vulva; Derived From Metastatic Site: Lymph Node Tissue: Vulva; Derived From Metastatic Site: Lymph Node<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

TK6 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-8015 TK6 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-8015<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

TO 166.M cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7776 TO 166.M cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7776<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

TO 175.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7779 TO 175.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7779<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Toledo cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2631 Toledo cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2631<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tongue Tongue<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

TOV-112D cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11731 TOV-112D cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11731<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

TOV-21G cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11730 TOV-21G cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11730<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

TT cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1803 TT cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1803<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Tu To cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1298 Tu To cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1298<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

TUR cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2367 TUR cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2367<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

U-118 MG cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-15 U-118 MG cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-15<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

U-138 MG cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-16 U-138 MG cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-16<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

U-2 OS cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-96 U-2 OS cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-96<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

U266B1 [U266] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 TIB-196 U266B1 [U266] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 TIB-196<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

U-87 MG cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-14 U-87 MG cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-14<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

U-937 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1593.2 U-937 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1593.2<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

U937-DC-SIGN cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3253 U937-DC-SIGN cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3253<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UACC-1179 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3127 UACC-1179 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3127<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UACC-1598 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3128 UACC-1598 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3128<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UACC-2087 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3180 UACC-2087 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3180<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UACC-2648 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3121 UACC-2648 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3121<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UACC-2727 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3192 UACC-2727 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3192<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UACC-3133 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2988 UACC-3133 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2988<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UACC-3199 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2983 UACC-3199 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2983<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UACC-462 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2989 UACC-462 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2989<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UACC-732 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3166 UACC-732 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3166<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UACC-812 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1897 UACC-812 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1897<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UACC-893 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1902 UACC-893 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1902<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

U-CH1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3217 U-CH1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3217<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

U-CH2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3218 U-CH2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3218<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UMC-11 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5975 UMC-11 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5975<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UM-Chor1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3270 UM-Chor1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3270<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UM-UC-3 [UMUC3] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1749 UM-UC-3 [UMUC3] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1749<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Unknown, Probably Lung Unknown, Probably Lung<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UPCI:SCC090 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3239 UPCI:SCC090 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3239<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UPCI:SCC152 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3240 UPCI:SCC152 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3240<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UPCI:SCC154 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3241 UPCI:SCC154 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3241<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Urinary Bladder Urinary Bladder<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

Uterus Uterus<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UWB1.289 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2945 UWB1.289 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2945<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

UWB1.289+BRCA1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2946 UWB1.289+BRCA1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2946<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

VA-ES-BJ cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2138 VA-ES-BJ cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2138<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

VCaP cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2876 VCaP cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2876<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

VK2/E6E7 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2616 VK2/E6E7 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2616<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

VMM1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3225 VMM1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3225<br/>【Organism物种来源】人源Homo sapiens, human  <br/>【Tissue组织来源】 <br/>【Cell Type细胞类型】  <br/>【Product Format产品状态】 frozen/live culture<br/>【Morphology细胞形态】  <br/>【Culture Properties细胞特性】 <br/>【Biosafety Level生物安全级别】 1/2<br/>Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.<br/><br/>【Disease细胞源疾病】<br/>【Age年龄】 <br/>【Gender性别】 Male/Female<br/>【Storage Conditions保存条件】 liquid nitrogen vapor phase<br/>【Karyotype染色体组型】 <br/>【Images细胞图片】 Cell Micrograph<br/>【Derivation衍生细胞】 ­<br/>【Clinical Data临床数据】 <br/>【Comments其他描述】 <br/>【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. <br/>【Subculturing传代方法】 <br/>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.<br/>Remove and discard culture medium.<br/>Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.<br/>Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).<br/>Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.<br/>Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.<br/>Add appropriate aliquots of the cell suspension to new culture vessels.<br/>Incubate cultures at 37°C.<br/>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br/>Medium Renewal: 2 to 3 times per week<br/>【Cryopreservation冻存条件】 <br/>【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO<br/>【Storage Temperature保存温度】 Liquid nitrogen vapor phase<br/>【Culture Conditions培养条件】 <br/>【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%<br/>【Temperature培养温度】 37°C<br/>【STR Profile图谱】 <br/>【Population Doub领 Time倍增殖时间】<br/>【References参考文献】<br/><br/>【Supplier细胞供应厂家】BioVector NTCC Inc.<br/>【Website网址】http://www.biovector.net<br/>

VMM15 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3227