ScriptCap™ Cap 1 Capping System C-SCCS1710

ScriptCap™ Cap 1 Capping System C-SCCS1710

产品简介：北京中北林格供应ScriptCap™ Cap 1 Capping System C-SCCS1710，中北林格是cellscript总代理，提供cellscript产品货号报价查询。其他品牌包括LGC Biosearch Technologies总代理，epicentre总代理，lucigen总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。

ScriptCap™ Cap 1 Capping System C-SCCS1710 品牌介绍：

CELLSCRIPT专注于再生医学，细胞再生学以及细胞疗法。细胞、组织或器官的移植ZL及修复中应用的细胞或生物体。CellScript还生产用于传递和翻译的合成修饰mRNA。

ScriptCap™ Cap 1 Capping System C-SCCS1710 规格详情名称：ScriptCap™ Cap 1 Capping System

ScriptCap™ Cap 1 Capping System C-SCCS1710 产品详情
A. Synthesis of Cap 0 RNA from Uncapped RNA 1. The protocol below was designed to build a cap 1 structure on 5' end of 50-60 µg of uncapped RNA. If a cap 0 structure is desired, replace the ScriptCap 2'-O-Methyltransferase in Step 3 with an equivalent volume of RNase-Free Water. Combine the following reagents:

2. Incubate at 65o C for 5-10 minutes, then transfer to ice. 3. While the heat-denatured RNA is coo领 on ice, prepare a “Cocktail” of the following reaction components together in a separate tube.

4. Just prior to starting the reaction, add the ScriptCap Capping Enzyme to the Cocktail from Step 3 and then combine this with the uncapped RNA solution from Step 1.

5. Incubate at 37o C for 30 minutes. 6. The Capped RNA can now be purified, or it can be 3' polyadenylated without purification by adding the reaction mixture directly to an A-Plus Poly(A) Polymerase Tai领 Kit reaction (sold separately).

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ScriptCap™ Cap 1 Capping System C-SCCS1710 关键词：
ScriptCap™ Cap 1 Capping System

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