pGAPZα A载体说明书
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pGAPZαA型号载体名称出品公司载体用途VJI0293pGAPZαAInvitrogen酵母表达载体Theglyceraldehyde-3-phosphatedehydrogenase(GAPDH)enzymeisconstitutivelyexpressedathighlevelsinmanyorganisms,includingPichiapastoris.Thepromoterofthegene(GAP)encodingtheGAPDHproteinhasrecentlybeencharacterizedandshowntoexpressrecombinantproteinstohighlevelsinPichiapastoris,dependingonthecarbonsourceused(Waterhametal.,1997).ThelevelofexpressionseenwiththeGAPpromoter(PGAP)canbeslightlyhigherthanthatobtainedwiththeAOX1promoter.ThepGAPZA,B,andCvectors(2.9kb)andpGAPZαA,B,andC(3.1kb)vectorsusetheGAPpromotertoconstitutivelyexpressrecombinantproteinsinPichiapastoris.ProteinscanbeexpressedasfusionstoaC-terminalpeptidecontainingthemycepitopefordetectionandapolyhistidinetagforpurificationonmetal-chelatingresin(i.e.ProBond).Inaddition,pGAPZαproducesproteinsfusedtoanN-terminalpeptideencodingtheSaccharomycescerevisiaeα-factorsecretionsignal.BothvectorsaresuppliedinthreereadingframestofacilitateinframecloningwiththeC-terminaltagand/ortheN-terminalsecretionsignal.Selectionofthesevectorsisbasedonthedominantselectablemarker,Zeocin,whichisbifunctionalinbothPichiaandE.coli.质粒图谱:上海起福生物科技有限公司联系人:杨建辉400电话:4006551678办公电话:021-50724187传真:021-50724961*8006手机:15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址:上海市浦东新区绣川路561号1102室
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