pRetroX-IRES-ZsGreen1载体说明书

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Clontech逆转录病毒载体pRetroX-IRES-ZsGreen1型号载体名称出品公司载体用途VNC0591pRetroX-IRES-ZsGreen1Clontech逆转录病毒载体DescriptionpRetroX-IRES-ZsGreen1isabicistronic,uorescent,retroviralvectorthatallowsbothageneofinterestandtheZsGreen1genetobetranslatedfromasinglebicistronicmRNA.pRetroXIRES-ZsGreen1isdesignedforefcientdeliveryandselection（byowcytometryorothermethods）ofstablytransducedmammaliancellsexpressingtheZsGreen1uorescentproteinandtheproteinofinterest.Thisvectorcanbeusedtoobtainstablecelllineswithouttimeconsumingdrugandclonalselection.ZsGreen1isderivedfromthereefcoralZoanthussp.greenuorescentprotein（ZsGreen）andiseasilydetectedwithstandardFITCltersets（1）.Bicistronicexpressionfromthisvectorisfacilitatedbytheencephalomyocarditisvirus（EMCV）internalribosomeentrysite（IRES）.ThisIRESfacilitatescap-independenttranslationfromaninternalstartsiteattheIRES/ZsGreen1junction（2）.ZsGreen1isahumancodonoptimizedZsGreenvariantthatencodesthebrightestcommerciallyavailablegreenuorescentprotein（1）.ThisretroviralvectorisderivedfromthepMINseriesofvectors（3,4）.Theseoptimizedvectorshavetheabilitytoproducehighviraltiters,expressgenesathighlevels,and,duetotheabsenceofretroviralcodingsequences,exhibitimprovedsafetyproles.Themultiplecloningsite（MCS）inpRetroX-IRES-ZsGreen1isbetweenthe5’MMLVLTRandtheIRESsequence.GenesclonedintotheMCSareexpressedasabicistronicmessagetranscribedfromthe5’LTR.pRetroX-IRES-ZsGreen1containsallofthenecessaryviralRNAprocessingelements;theseincludethe5’and3’LTRs,thepackagingsignal（ψ）,andthetRNAprimer-bindingsite.Forsafetyreasons,however,thevectorlacksthestructuralgenes（gag,pol,andenv）necessaryforretroviralparticleformationandreplication.pRetroX-IRES-ZsGreen1containsaColE1originofreplication,andanE.coliAmprgeneforpropagationandselectioninbacteria.质粒图谱:上海起福生物科技有限公司联系人：杨建辉400电话：4006551678办公电话：021-50724187传真：021-50724961*8006手机：15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址：上海市浦东新区绣川路561号1102室
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