Matchmaker Library Construction & Screening Kits （pGBKT7，pGADT7，pGBKT7-53，pGADT7-T，pGBKT7-Lam）

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pGBKT7-53产品参数:BacteriaResistance：KanamycinMammalianSelection：-载体抗性:卡那霉素（Kanamycin）载体描述:载体特点：与pGADT7-T构成MatchmakerTwo-HybridSystem3的双杂交正对照。pGBKT7-53encodesafusionofthemurinep53protein（a.a.72390）andtheGAL4DNA-BD（a.a.1147）.Themurinep53cDNA（GenBankAccession#K01700）wasclonedintopGBKT7attheEcoRIandBamHIsites.Thep53insertwasderivedfromtheplasmiddescribedinIwabuchietal.（1993）;plasmidmodificationwasperformedatClontech.pGBKT7-53hasnotbeensequenced.质粒图谱:pGBKT7-Lam产品参数:BacteriaResistance：KanamycinMammalianSelection：-载体抗性:卡那霉素（Kanamycin）载体描述:载体特点：MatchmakerTwo-HybridSystem3的负对照。pGBKT7-LamisanegativecontrolplasmidthatencodesafusionofthehumanlaminCprotein（a.a.66230）andtheGAL4DNA-BD（a.a.1147）.ThelaminCcDNAinsert（GenBankAccession#M13451）wasderivedfromtheplasmidreferencedinBarteletal.（1993a）.PlasmidmodificationwasperformedatBDBiosciencesClontech.YeastcotransformedwithpGBKT7-LamandpGADT7-RecT,provideameasureofthebackgroundthatisduetofalse-positivetwo-hybridinteractions.pGBKT7-Lamhasnotbeensequenced.质粒图谱:
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