真菌-酵母细胞内氧化应激活性氧高质荧光测定试剂盒文献

收藏 评价 举报

• 分享

AbstractBackground:PolyporusumbellatussclerotiahavebeenusedasadiureticagentinChinaforovertwothousandyears.AshortageofthenaturalP.umbellatushaspromptedresearcherstoinducesclerotialformationinthelaboratory.Methodology/PrincipalFinding:P.umbellatuscultivationinasawdust-basedsubstratewasinvestigatedtoevaluatetheeffectoflowtemperatureconditionsonsclerotialformation.Aphenol-sulfuricacidmethodwasemployedtodeterminethepolysaccharidecontentofwildP.umbellatussclerotiaandmyceliaandsclerotiagrowninlow-temperaturetreatments.Inaddition,reactiveoxygenspecies(ROS)content,expressedasthefluorescenceintensityofmyceliaduringsclerotialdifferentiationwasdetermined.AnalysisofROSgenerationandsclerotialformationinmyceliaaftertreatmentwiththeantioxidantssuchasdiphenyleneiodoniumchloride(DPI),apocynin(Apo),orvitaminCwerestudied.Furthermore,macroscopicandmicroscopiccharacteristicsofsclerotialdifferentiationwereobserved.Sclerotiawerenotinducedbycontinuouscultivationat25uC.Thepolysaccharidecontentoftheartificialsclerotiais78%ofthatofwildsclerotia.Inthelow-temperaturetreatmentgroup,thefluorescentintensityofROSwashigherthanthatoftheroomtemperature(25uC)groupwhichdidnotinducesclerotialformationallthroughthecultivation.TheantioxidantsDPIandAporeducedROSlevelsanddidnotinducesclerotialformation.Althoughtheconcentration-dependenteffectsofvitaminC(515mgmL21)alsoreducedROSgenerationandinhibitedsclerotialformation,usingalowconcentrationofvitaminC(1mgmL21)successfullyinducedsclerotialdifferentiationandincreasedROSproduction.Conclusions/Significance:ExposuretolowtemperaturesinducedP.umbellatussclerotialmorphogenesisduringcultivation.LowtemperaturetreatmentenhancedROSinmycelia,whichmaybeimportantintriggeringsclerotialdifferentiationinP.umbellatus.Moreover,theapplicationofantioxidantsimpairedROSgenerationandinhibitedsclerotialformation.OurfindingsmayhelptoprovidenewinsightsintothebiologicalmechanismsunderlyingsclerotialmorphogenesisinP.umbellatus.Citation:XingY-M,ZhangL-C,LiangH-Q,LvJ,SongC,etal.(2013)SclerotialFormationofPolyporusumbellatusbyLowTemperatureTreatmentunderArtificialConditions.PLoSONE8(2):e56190.doi:10.1371/journal.pone.0056190Editor:PratibhaV.Nerurkar,CollegeofTropicalAgricultureandHumanResources,UniversityofHawaii,UnitedStatesofAmericaReceivedAugust16,2012;AcceptedJanuary7,2013;PublishedFebruary20,2013Copyright:2013Xingetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited.Funding:TheresearchwasfinanciallysupportedbytheNationalNaturalSciencesFoundationofChina(No.30830117,31201666),theInternationalScienceandTechnologyCooperationProjectsofChina(No.2011DFA31260)andtheNationalResearchFoundation(NRF20110016999).Thefundershadnoroleinstudydesign,datacollectionandanalysis,decisiontopublish,orpreparationofthemanuscript.CompetingInterests:Theauthorshavedeclaredthatnocompetinginterestsexist.*E-mail:sxguo2006@yahoo.com.cn(SXG);cl王@implad.ac.cn(CLW)
标签：

• CCK-8试剂盒说明书

  CCK-8试剂盒 WST-8 细胞增殖 细胞毒性，是一种基于WST-8的广泛应用于细胞增殖和细胞毒性的快速高

• 支原体PCR检测试剂盒说明书

  支原体PCR检测试剂盒 灵敏度高 使用方便 涵盖所有支原体种类，能在2-3小时内获得可靠的结果。特点：①特异

• 活性氧化铝比表面积的研究与控制

  活性氧化铝比表面积的研究与控制

• Tecan核酸提取与纯化套餐又添新成员，老牌试剂盒Macherey

  Tecan核酸提取与纯化套餐又添新成员，老牌试剂盒Macherey

• 水体中活性氧检测解决方案

  水体中活性氧检测解决方案

• 美国Abraxis公司三聚氰胺检测试剂盒检测方法

  美国Abraxis公司三聚氰胺检测试剂盒检测方法

• -内酰胺酶检测试剂盒

  -内酰胺酶检测试剂盒

• 鲤鱼卵黄蛋白原ELISA检测试剂盒

  鲤鱼卵黄蛋白原ELISA检测试剂盒

• 牛奶中抗生素残留检测试剂盒的研制及应用

  牛奶中抗生素残留检测试剂盒的研制及应用

• 小鼠糖皮质激素受体(GR-)elisa试剂盒使用说明书

  小鼠糖皮质激素受体(GR-)elisa试剂盒使用说明书