INTRODUCTION Preparative LC coupled with mass spectrometry (MS) is a well-established method for purifying target compounds. The VERITY® 271 LCMS system (Figure 1) leverages the separating power of HPLC with the detection capabilities of MS to isolate fractions with high purity and achieve excellent recovery for target molecules. The system incorporates signal data from both ultraviolet (UV) and mass-based detectors.
INTRODUCTION
Preparative LC coupled with mass spectrometry (MS) is a well-established method for purifying target compounds. The VERITY® 271 LCMS system (Figure 1) leverages the separating power of HPLC with the detection capabilities of MS to isolate fractions with high purity and achieve excellent recovery for target molecules. The system incorporates signal data from both ultraviolet (UV) and mass-based detectors. [详细]
INTRODUCTION
Liquid chromatography coupled with mass spectrometry (LCMS) is widely used in both analytical and preparative chemistry applications. In this process, compounds in a complex mixture are separated based on their size or chemical properties using LC, and the mass signal from the MS detector is used to confirm compound identity and trigger fraction collection. [详细]
APPLICATION BENEFITS
The necessity to perform purification with subsequent analysis via HPLC to determine the composition of collected fractions consumes scientists’ time and money. Utilization of multiple instruments often requires the user to learn multiple software applications. This causes scientists to spend more time learning software functionalities, and less time applying their knowledge to scientific discoveries.[详细]
INTRODUCTION
This technical note describes a simple plumbing scheme that allows optimization for both mass-directed collection of fractions, and subsequent automated reinjection of those fractions using off-the-shelf Gilson instrumentation. The dual system (prep and analytical) utilized a diode array detector and a VERITY® 1900 MS Detector (Figure 1 ). A 2-position, 10-port VALVEMATE® II was employed to switch the pump to the injector, the column to the detector, and to maintain the UV detector,[详细]
APPLICATION BENEFITS
Mycotoxins are toxic compounds produced by fungi that are harmful to humans and animals. Patulin, found in rotting apples, is a compound that is regulated in many countries. Food safety testing for patulin is crucial to limit exposure; however, many sample cleanup methods do not remove closely related compounds, which can lead to false positive results.[详细]
TN221-01-使用Gilson VERITY® 271 LCMS质谱引导制备色谱系统分离多肽混合物（英文）
混合物的分离提纯方法归纳
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