微流控细胞压缩系统

微流控细胞压缩系统可以通过调节正气压力对体外组织样本或3D细胞培养物进行循环或静态压缩。FX-5000C压缩系统使用 BioPress培养板在活塞和固定压板之间压缩样品。它最多可以施加14 磅的压力。凭借使用 FX-5000C加压系统，研究人员可以在体外观察模拟体内条件的生化变化和细胞信号传导。

微流控细胞压缩系统的工作原理

BioPress孔中对组织样本进行压缩的示意图

​

微流控细胞压缩系统FX-5000C的优点：
1）产生高达14磅作用力的正气压力（最小力分辨率为0.1磅力）
2）在来自肌肉、肺、心脏、血管、皮肤、肌腱、韧带、软骨和骨骼的3D构建或组织样本中模拟的体内组织应变和频率；
3）使用BioPress培养板或StagePresser显微镜设备在活塞和固定压板之间压缩样品
4）在一个方案内编程多个频率、幅度和波形变化，频率范围从0.01到5.0Hz；
5）一个FX-5000微流控压缩系统可以驱动四个FlexLink控制器
6）可在Windows7系统上运行FlexSoft FX-5000操作软件

参考文献
Altalhi W, Sun X, Sivak JM, Husain M, Nunes SS. Diabetes impairs arterio-venous specification in engineered vascular tissues in a perivascular cell recruitment-dependent manner. Biomaterials 119:23-32, 2017.

Cevallos M, Riha GM, Wang X, Yang H, Yan S, Li M, Chai H, Yao Q, Chen C. Cyclic strain induces expression of specific smooth muscle cell markers in human endothelial cells. Differentiation 74(9-10):552-561, 2006.

Freese C, Anspach L, Deller RC, Richards SJ, Gibson MI, Kirkpatrick CJ, Unger RE. Gold nanoparticle interactions with endothelial cells cultured under physiological conditions. Biomater Sci 5(4):707-717, 2017.

Gao M, Wu S, Ji J, Zhang J, Liu Q, Yue Y, Liu L, Liu X, Liu W. The influence of actin depolymerization induced by Cytochalasin D and mechanical stretch on interleukin-8 expression and JNK phosphorylation levels in human retinal pigment epithelial cells. BMC Ophthalmol 17(1):43, 2017.

Harris C, Rushwan S, Wang W, Thorpe S, Thompson C, Peacock J, Knight M, Gooptu B, Greenough A. P07 Interleukin response to cyclical mechanical stretch with models of different neonatal ventilation modes. Archives of Disease in Childhood 102:A4, 2017.

Freeman SA, Christian S, Austin P, Iu I, Graves ML, Huang L, Tang S, Coombs D, Gold MR, Roskelley CD. Applied stretch initiates directional invasion through the action of Rap1 GTPase as a tension sensor. J Cell Sci 130(1):152-163, 2017.

Glaeser JD, Salehi K, Kanim LE, NaPier Z, Kropf MA, Cuellar J, Sheyn D, Bae HW. Treatment with the NFkB inhibitor reduces overloading-induced MMP expression in human nucleus pulposus cells. The Spine Journal 17(10):S127, 2017.

Klymenko Y, Wates RB, Weiss-Bika H, Lombard R,Liu Y, Campbell L, Kim O, Wagner D, Ravosa MJ, Stack MS. Modeling the effect of ascites-induced compression on ovarian cancer multicellular aggregates. Dis Model Mech.2018 Sep 25; 11 (9).

Ackermann P, Schizas N, Bring D, Li J, Andersson T, Fahlgren A, Aspenberg P. Compression therapy promotes tissue repair and biomechanical properties during immobilization. J Bone Joint Surg Br 94B (Supp XXXVII) 89, 2012.