Effective inhibition of the degradation of RNA by eukaryotic RNases in a variety of applications, including cDNA synthesis, RT-PCR, and in vitro transcription and translation.
真核细胞核糖核酸酶有效YZRNA降解的各种应用,包括cDNA合成,RT-PCR以及 体外 转录和翻译。
A potent affinity for RNases (Ki >10-14 M) ensures rapid inhibition even when trace amounts of RNase are present.
Free of detectable RNase or DNase activity, and mammalian DNA.
Does not interfere with enzymes commonly used to prepare or analyze RNA.
Less sensitive to oxidation than traditional RNase inhibitors.
对于RNA酶甲强亲和性(K 我 > 10 -14 M)确保了快速YZ,即使存在RNA酶的痕量存在。
没有可检测的RNase或DNase活性,以及哺乳动物DNA。
不干扰通常用于制备或分析RNA的酶。
不如传统的RNaseYZ剂对氧化敏感。
ScriptGuard™ RNase Inhibitor will now be sold as RiboGuard™ RNase Inhibitor. There is no change in product specification or price, only product name and catalog numbers have changed. (SRI6325 is now RG90925, and SRI6310K is now RG90910K.)
ScriptGuard™RNaseYZ剂现在将作为RiboGuard™RNaseYZ剂出售。产品规格或价格没有变化,只有产品名称和目录号已更改。(SRI6325现在是RG90925,而SRI6310K现在是RG90910K。)
Applications
RiboGuard™ RNase Inhibitor is the best defense against common RNases, including RNase A, RNase B, and RNase C. This recombinant RNase inhibitor protein provides reliable protection of valuable RNA samples by binding strongly to RNases in a 1:1 ratio. Advanced purification protocols and extensive quality control tests ensure RiboGuard RNase Inhibitor is free of unwanted contaminants that can plague other commercially available preparations of RNase inhibitors.
RiboGuard™RNaseYZ剂是针对常见RNase(包括RNase A,RNase B和RNase C)的**防御。这种重组RNaseYZ剂蛋白通过以1:1的比例与RNase牢固结合,为有价值的RNA样品提供了可靠的保护。先进的纯化方案和广泛的质量控制测试确保RiboGuard RNaseYZ剂不含有害污染物,这些污染物会困扰其他市售的RNaseYZ剂制剂。
Benefits
Unit Definition: One unit of RiboGuard RNase Inhibitor results in 50% inhibition of 5 ng of RNase A. Activity is measured by the inhibition of hydrolysis of 2,3´-cyclic monophosphate by RNase A.
单位定义: 一单位RiboGuard RNaseYZ剂对5 ng RNase A产生50%的YZ作用。通过YZRNase A对2,3′-环一磷酸的水解来测量活性。
Storage Buffer: 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 100 mM NaCl, and 10 mM DTT.
储存缓冲液: 含有50 mM Tris-HCl(pH 7.5),0.1 mM EDTA,100 mM NaCl和10 mM DTT的50%甘油溶液。
Quality Control: RiboGuard RNase Inhibitor is free of detectable DNA exo- and endonuclease, and RNase activities.
质量控制: RiboGuard RNaseYZ剂不含可检测的DNA外切核酸酶和内切核酸酶以及RNase活性。
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仪企号北京中北林格科技发展有限公司
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