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ExoView外泌体全面表征试剂盒

品牌：NanoView
规格型号： 0
产地：美国
样册：暂无
供应商报价：面议

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详细介绍

ExoView外泌体全面表征试剂盒

外泌体计数、粒径、蛋白表达、蛋白共定位一次完成

检测样本类型	对细胞培养上清、血浆、血清、尿液、脑脊液、唾液等生物样本中的外泌体直接进行分析
捕获抗体种类	anti-CD81, anti-CD9, anti-CD63, 同型IgG对照；可自定义
单次上样体积	35 µl稀释样本
重复检测数目	3复孔
荧光抗体种类	CD9（Blue）/ CD81（Green）/ CD63（Red）

实验原理

① 35 μL外泌体样品滴加在芯片上孵育；

② 预先包被的抗体特异结合外泌体表面蛋白以捕获外泌体；

③ 再使用荧光抗体特异性标记需要表征的标记物；

④ ZH用ExoView R100检测外泌体粒径、计数、蛋白表达（CD9，CD81，CD63等）及共定位。

检测流程

产品类别

产品货号	产品名称
EV-TETRA-C	人外泌体检测试剂盒
EV-TETRA-P	人血浆外泌体检测试剂盒
EV-TETRA-M2	鼠外泌体检测试剂盒
EV-TETRA-C-CAR	人外泌体内容物检测试剂盒
EV-TETRA-P-CAR	人血浆外泌体内容物检测试剂盒
EV-TC-FLEX	自由捕获人外泌体检测试剂盒
EV-TP-FLEX	自由捕获人血浆外泌体检测试剂盒
EV-TC-FLEX-CAR	自由捕获人外泌体内容物检测试剂盒
EV-TP-FLEX-CAR	自由捕获人血浆外泌体内容物检测试剂盒
EV-TM-FLEX	自由捕获鼠外泌体检测试剂盒
EV-TM-FLEX-CAR	自由捕获鼠外泌体内容物检测试剂盒
EV-FLEX-2	自由捕获外泌体检测试剂盒
EV-FLEX-2 -CAR	自由捕获外泌体内容物检测试剂盒
EV-CTETRA-1/2/3	人外泌体检测试剂盒+1/2/3个自定义捕获抗体
EV-CTETRA-1/2/3-CAR	人外泌体内容物检测试剂盒+1/2/3个自定义捕获抗体
EV-CUST-1/2/3/4/5/6	自定义1/2/3/4/5/6抗体捕获外泌体检测试剂盒
EV-CUST-1/2/3/4/5/6-CAR	自定义1/2/3/4/5/6抗体捕获外泌体内容物检测试剂盒

试剂盒特点

特异性捕获

芯片上可包被多达6种捕获抗体，特异性捕获含特定蛋白标记物的外泌体。

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JD阳性外泌体计数

芯片捕获外泌体后，可通过SP-IRIS技术直接检测样品中外泌体的数量。

单个外泌体蛋白共定位分析

检测每个外泌体的荧光信号并进行统计，可获得荧光共定位信息，用于分析样品中不同表型外泌体的比例（如右图所示）。

无需纯化

使用抗体捕获模式，防止样品中杂质影响结果，可直接检测血液、尿液和细胞培养液中的外泌体，未纯化样品的测量结果与纯化后基本一致（如右图所示）。

粒径分辨率高

高精度SP-IRIS技术，可检测≥50 nm的外泌体，测量结果与电子显微镜检测结果基本一致，并统计生成外泌体的粒径分布结果（如右图所示）。

可检测外泌体内容物

试剂盒配套相应的穿膜剂，可穿透外泌体并对外泌体内容物进行染色并检测，未穿膜时只能检测到跨膜蛋白CD9的荧光信号，穿膜后即可检测到外泌体内容物Syntenin的表达（如右图所示）。

测试数据

外泌体荧光数量统计

外泌体粒径检测

外泌体内容物检测

荧光强度与粒径关系

荧光共定位分析

发表文章

• Andras Saftics.(2021) Data evaluation for surface-sensitive label-free methods to obtain real-time kinetic and structural information of thin films: A practical review with related software packages. Advances in Colloid and Interface Science.

• Kyoung-Won Ko.(2021) Integrated Bioactive Scaffold with Polydeoxyribonucleotide and Stem-Cell-Derived Extracellular Vesicles for Kidney Regeneration. ACS Nano.

• Tanina Arab. (2021) Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single‐particle analysis platforms. Journal of Extracellular Vesicles.

• Niaz Z.Khan.(2021) Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential. Brain, Behavior, and Immunity.

• Dario Brambilla. (2021) EV Separation: Release of Intact Extracellular Vesicles Immunocaptured on Magnetic Particles. Analytical Chemistry.

• Enkhtuya Radna. (2021) Extracellular vesicle mediated feto-maternal HMGB1 signaling induces preterm birth. Lab on a Chip.

• Li, M., Soder. (2021) WJMSC‐derived small extracellular vesicle enhance T cell suppression through PD‐L1. Journal of Extracellular Vesicles.

• Crescitelli, R. (2021) Isolation and characterization of extracellular vesicle subpopulations from tissues. Nature protocols.

• Berger, A. (2021). Local administration of stem cell-derived extracellular vesicles in a thermoresponsive hydrogel promotes a pro-healing effect in a rat model of colo-cutaneous post-surgical fistula. Nanoscale.

• Vidal, M. (2020) Exosomes and GPI-anchored proteins: Judicious pairs for investigating biomarkers from body fluids. Advanced drug delivery reviews.

• K Cho, H Kook.(2020)Study of immune-tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA-G from semiallograft immune tolerance during pregnancy. Journal of Extracellular Vesicles.

• Maximillian A. Rogers.(2020)Annexin A1–dependent tethering promotes extracellular vesicle aggregation revealed with single–extracellular vesicle analysis. Cell Biology.

• Annette M. Marleau.(2020)Targeting tumor-derived exosomes using a lectin affinity hemofiltration device. Cancer Research.

• Alessandro Gori.(2020)Membrane-Binding Peptides for Extracellular Vesicles On-Chip Analysis. Journal of Extracellular Vesicles.

• Rossella Crescitelli.(2020)Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation. Journal of Extracellular Vesicles.

• Maria S. Panagopoulou.(2020) Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence. Journal of Extracellular Vesicles.

• WeiYan.(2020) Immune Cell-Derived Exosomes in the Cancer-Immunity Cycle. Trends in Cancer.

• Daniel Bachurski. (2019) Small RNA Sequencing across Diverse Biofluids Identifies Optimal Methods for exRNA Isolation. Cell.

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