His Tag琼脂糖填料

固相化金属离子亲和层析（IMAC）是近30年来出现的一种亲和层析技术它于1975年首先被Porath和他的合作者们成功用于分离纯化人血清蛋白。并在此后的三十年里迅速发展。该方法是利用蛋白质表面暴露的一些氨基酸残基和载体之上的金属离子之间的相互作用而进行的亲和纯化。它以配基简单、吸附量大、分离条件温和、通用性强等特点，逐渐成为分离纯化蛋白质等生物工程产品最有效的技术之一。

ABT 公司提供能用于不同条件下的产品（可针对天然和变性的蛋白),还有针对不同的残基的产品（高度和一般活化的磁珠），不同的阳离子：Ni2+, Co2+, Zn2+, Cu2+或者无金属的产品，还有不同的形式的产品（大量树脂，预装柱和一些柱芯）

树脂：

? Different grades of activation. High Density (High

binding capacity) & Low Density (High selectivity/

specificity)

? Resins charged with Ni2+, Co2+, Zn2+, Cu2+ or metal

free

? Pack size: 25 ml, 100 ml & 500 ml

BEAD GEOMETRY & SIZE Spherical, Standard: ~ 50 - 150 μm

CROSSLINKED Yes

AUTOCLAVABLE 121o C, 30’

BEAD AGAROSE % 6%

ACTIVATING AGENT： Epichlorohydrin

MATRIX Stable in all commonly used reagents

PRODUCT： HIGH Density/ LOW Density

METAL FREE/NICKEL/ZINC/COBALT METAL FREE/NICKEL/ZINC/COPPER

BINDING/LOADING CAPACITY20-40 / 5-20

(μmol Me2+/ml gel)

ANTIMICROBIAL AGENT 20% Ethanol

STORAGE TEMPERATURE 2 - 8o C

预装柱：

For gravity flow purifications.

? No need for purification systems

? Contains 1 or 5 ml of gel

? Resins charged with Ni2+, Co2+, Zn2+ and Cu2+

COLUMN MATERIAL: Polypropylene column and Polyethylene frit

BED VOLUME :1 ml / 5 ml (XL)

CROSSLINKED: Yes

BEAD AGAROSE % 6%

ANTIMICROBIAL AGENT: 20% Ethanol

STORAGE TEMPERATURE 2 - 8o C

NI-NTA琼脂糖磁珠

Resin for work in reducing conditions because of the

four metal-binding sites on the chelate.

? One step purification

? High capacity

? Purification under native or denaturing conditions

? Minimum metal binding

? Pack size: 25 ml, 100 ml & 500 ml

BEAD GEOMETRY： Spherical, Standard: ~ 50 - 170

μm

CROSSLINKED: Yes

LIGAND Nitrilotriacetic acid (NTA)

CAT. No. 6BCL-NTANi-X

BINDING CAPACITY > 50 mg/ ml gel

ANTIMICROBIAL AGENT 30% Ethanol

STORAGE TEMPERATURE 4 - 8o C