详细介绍
概述
| 产品描述 | E. coli expressed, His-tagged (C-ter) Active Human HMGB1 recombinant protein. |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FuncSt, SDS-PAGE |
| 靶点名称 | HMGB1 |
| 物种 | Human |
| A.A. 序列 | MGKGD PKKPR GKMSS YAFFV QTCRE EHKKK HPDAS VNFSE FSKKC SERWK TMSAK EKGKF EDMAK ADKAR YEREM KTYIP PKGET KKKFK DPNAP KRPPS AFFLF CSEYR PKIKG EHPGL SIGDV AKKLG EMWNN TAADD KQPYE KKAAK LKEKY EKDIA AYRAK GKPDA AKKGV VKAEK SKKKK EEEED EEDEE DEEEE EDEED EDEEE DDDDE with polyhistidine tag at the C-terminus. |
| 表达系统 | E. coli |
| 活动 | Active |
| 活动说明 | Measure by its ability to induce TNF alpha in Raw264.7 cells. for this effect is < 10 μg/ml. This protein also induces TNF alpha releasing from human A549 cultured cells based on customer feedback. |
| 別名 | HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3 |
属性
| 形式 | Powder |
|---|---|
| 纯化 | Ni-NTA chromatography. |
| 纯化说明 | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 98% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 8.0) |
| 重建 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| 存放说明 | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | GeneID: 15289 Mouse HMGB1 GeneID: 3146 Human HMGB1 Swiss-port # P09429 Human High mobility group protein B1 Swiss-port # P63158 Mouse High mobility group protein B1 |
|---|---|
| 基因名称 | HMGB1 |
| 全名 | high mobility group box 1 |
| 背景介绍 | HMGB1 is a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015] |
| 生物功能 | HMGB1 is a DNA binding protein. It associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). [UniProt] |
| 细胞定位 | Nucleus. Chromosome. Cytoplasm. Secreted. Cell membrane; Peripheral membrane protein; Extracellular side. Endosome. Endoplasmic reticulum-Golgi intermediate compartment. Note=In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus. Translocates from the nucleus to the cytoplasm upon autophagy stimulation. Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes. [UniProt] |
| 产品亮点 | Related products: HMGB1 antibodies; HMGB1 ELISA Kits; HMGB1 Duos / Panels; HMGB1 recombinant proteins; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 Total solution for HMGB1 research HMGB1 in inflammation Inflammatory Cytokines HMGB1 ELISA Kit for your research Detecting the DAMPs in cancer therapy by HMGB1 ELISA kit New HMGB1 neutralizing antibody is released Detecting exosomal HMGB1 for ICD research Related poster download: HMGB1 Pathway.pdf |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460). Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity). Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so). Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity). In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex; reduces binding to heparin and proinflammatory activities (By similarity). [UniProt] |
主要用途
HMGB1 是一种多功能蛋白，在炎症与肿瘤均扮演重要角色。HMGB1 可做为损伤相关分子模式 (DAMP) 分子诱发炎症；然而过量 HMGB1 所诱发的高强度炎症反而会导致机体组织损伤。另外，肿瘤细胞分泌的 HMGB1 可促进血管生成与免疫抑制，从而推进肿瘤进展。
