详细介绍
| 货号: 26925 |
| 基因符号: ARL3 |
| 描述: Anti-Arl3-GTP Mouse Monoclonal Antibody |
| 背景介绍: Arl3 (Arf-like 3) is an Arf family protein. Arl3 binds microtubules to alter specific aspects of cytokinesis. It has been proposed that retinitis pigmentosa 2 functions in concert with Arl3 to link the cell membrane and the cytoskeleton in photoreceptors as part of the cell signaling or vesicular transport machinery. |
| 免疫原: Recombinant Arl3 protein (active form) |
| 经过测试的应用: IP, IHC, IF |
| 推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| 浓度: 1 mg/ml |
| 宿主: |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Arl3 antibody recognizes active Arl3-GTP from vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
免疫沉淀/蛋白质印迹:  Arl3 Activation Assay. Purified His-tagged Arl3 proteins (Cat. #10152) were immunoprecipitated with the anti-active Arl3 monoclonal antibody (Cat. #26925) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti-Arl3 monoclonal antibody (Cat. #21070). Input controls are shown in the bottom panel. |
主要用途
GTP酶是GTP/GDP结合蛋白。被细胞表面受体(GPCR)激活后, 它能调节下游效应器的功能。它可以结合GTP、水解GTP，因此在激活的GTP结合状态和非激活的GDP结合状态之间切换。它具有内在的GTP酶活性，可将GTP水解为GDP，从而使G蛋白失活。我们精心筛选的小鼠单克隆抗体能捕捉到与GTP结合的具有活性的GTP酶。它们已被用于通过免疫组织化学监测细胞和组织中 G 蛋白的活化和分布。
- 产品分类
- 品牌分类
-
仪企号
武汉费斯德生物科技有限公司
-
友情链接
