仪器网

欢迎您: 免费注册 仪器双拼网址:www.yiqi.com
首页-资讯-资料-产品-求购-招标-品牌-展会-行业应用-社区-供应商手机版
官方微信
中国仪器网• 行业应用
仪器网/ 行业应用/ 解决方案/ QCM, strand displacement reaction

QCM, strand displacement reaction

点击这里给我发消息
内容节点
概述
实验/设备条件
样品提取
实验/操作方法
实验结果/结论
仪器/耗材清单
Toehold-mediated strand displacement reaction
(SDR) is first introduced to develop a simple quartz
crystal microbalance (QCM) biosensor without an enzyme or
label at normal temperature for highly selective and sensitive
detection of single-nucleotide polymorphism (SNP) in the p53
tumor suppressor gene. A hairpin capture probe with an
external toehold is designed and immobilized on the gold
electrode surface of QCM. A successive SDR is initiated by the
target sequence hybridization with the toehold domain and
ends with the unfolding of the capture probe. Finally, the
open-loop capture probe hybridizes with the streptavidincoupled
reporter probe as an efficient mass amplifier to enhance the QCM signal. The proposed biosensor displays remarkable
specificity to target the p53 gene fragment against single-base mutant sequences (e.g., the largest discrimination factor is 63 to
C−C mismatch) and high sensitivity with the detection limit of 0.3 nM at 20 °C. As the crucial component of the fabricated
biosensor for providing the high discrimination capability, the design rationale of the capture probe is further verified by
fluorescence sensing and atomic force microscopy imaging. Additionally, a recovery of 84.1% is obtained when detecting the
target sequence in spiked HeLa cells lysate, demonstrating the feasibility of employing this biosensor in detecting SNPs in
biological samples. Q-Sense全自动八通道石英晶体微天平
相关产品
相关解决方案
热门解决方案
最新解决方案
在线留言
官方微信

仪器网微信服务号

扫码获取最新信息


仪器网官方订阅号

扫码获取最新信息

在线客服

咨询客服

在线客服
工作日:  9:00-18:00
联系客服 企业专属客服
电话客服:  400-822-6768
工作日:  9:00-18:00
订阅商机

仪采招微信公众号

采购信息一键获取海量商机轻松掌控