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Dionex Acclaim 300 C18 Column 生物色谱柱
- 品牌:美国戴安
- 型号: 美国戴安 P/N: 059457
- 产地:美洲 美国
- 供应商报价:面议
- 上海希言科学仪器有限公司 更新时间:2024-09-11 17:26:09
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详细介绍
Acclaim 300 C18 Protein and Peptide Column
High-Resolution Separation of Proteins and Peptides
The Acclaim? 300 features 3 ?m silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.
- Designed for high-resolution peptide mapping and protein separations
- High-efficiency 3 ?m spherical silica substrate
- High-performance bonding chemistry on 300 ? pore silica
- Application tested for suitablility in peptide mapping
- Reproducible for dependable results
- LC/MS compatible
Manufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tai领 effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions.
The use of a 3 ?m silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 ?m column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.
Acclaim PepMap for TFA-Free Peptide Mapping Specifications Column type Nano Capillary Micro Column i.d. 75 ?m 300 ?m 1.0 mm Length 50, 150, 250, and 500 mm 50, 150, and
250 mm50, 150, and
250 mmRecommended Flow Rate 200 nL/min 4 ?L/min 40 ?L/min Packing Material C18, 100 ?,
3 ?mC18, 100 ?,
3 ?mC18, 100 ?,
3 ?mC18, 300 ?,
5 ?mC18, 300 ?,
5 ?mC18, 300 ?,
5 ?mC8, 100 ?,
3 ?mC8, 100 ?,
3 ?mC8, 100 ?,
3 ?mC8, 100 ?,
5 ?mC8, 100 ?,
5 ?mC8, 100 ?,
5 ?mC4, 300 ?,
5 ?mC4, 300 ?,
5 ?mC4, 300 ?,
5 ?mColumn Fused-silica Glass Lined Tubing Stainless steel Column Inlet 1/16-in.
PEEK sleeve adapter
(provided)
nanoViper (optional)1/16-in.
universal fitting
nanoViper (optional) 50?m i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.
PEEK tubing
nanoViper (optional) 75?m i.d. PEEK sheathed fused silica with nanoViper fittingsColumn Outlet Fused-silica
25-?m i.d./ 280-?m o.d.Fused-silica
75-?m i.d./ 280-?m o.d1/16-in.
PEEK tubing
nanoViper (optional) 75?m i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet 1-m Length The standard particle size is nominally 3 ?m. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters; standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 ?m, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.
Analytical Columns Acclaim 300, C18, 3 ?m Analytical (2.1 x 50 mm) 060263 Acclaim 300, C18, 3 ?m Analytical (2.1 x 150 mm) 060264 Acclaim 300, C18, 3 ?m (3.0 x 150 mm) 063684 Acclaim 300, C18, 3 ?m Analytical (4.6 x 50 mm) 060265 Acclaim 300, C18, 3 ?m Analytical (4.6 x 150 mm) 060266 Guard Columns Acclaim 300,C18, 3?m, Guard Cartridges, (2.1 x 10mm), 2 ea; requires holder 069580 069690 PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA 069697 Hardware Acclaim SST Guard Cartridge Holder V-1 059456 Guard to Analytical Column Coupler 059457 Guard Kit (Holder and Coupler) 059526 Acclaim SST Guard Cartridge Holder V-2 069580 Acclaim Guard Kit (Holder and coupler) V-2 069707 Guard to Analytical Column Coupler V-2 074188 Micro and Nano Columns Acclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper 164567 75 ?m i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 ?m, 300?, nanoViper 164708 300 ?m i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 ?m, 100?, nanoViper 164716 300 ?m i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 ?m, 300?, nanoViper 164718 Acclaim PepMap300, C18, 5 ?m, 300 ? Wide Pore, 1.0 mm x 5 cm 163584 Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper 164572
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