HoloMonitorM4
M4激光全息细胞成像分析系统，配备和M3同样强大的细胞图像分析软件，实现在细胞正常生
长环境下的非侵入，非标记活细胞成像及分析。
M4仪器非常精巧，可以放置在培养箱中，实现非常wan美的Timelapse（延时成像）分析。同时，
测量并分析细胞成像，细胞计数，细胞面积和体积等形态学参数。

M4性能优势
*实时3D成像
*直接测量贴壁细胞
*非标记，无需染色，无光毒性
*细胞面积和体积分析
*长期time-lapse成像分析
*细胞跟踪功能
*体积小巧，可直接放在培养箱中

下面一组Time-lapse图片展示了人巨噬细胞吞噬酵母细胞时形态的改变

如下一组Time-lapse图片展示了人MCF-10A乳腺细胞（30分钟间隔拍摄）

技术参数：
图像类型:定量相位图像
CCD相机:1280×1024像素,8or10bits(全息图)
光源:单色激光(全息模式)
物镜:20x全息
计算机:处理器IntelCorei7,Memory6GB,Harddrive1TB,GraphicsNVidia
1GB,Screen1920x1080,FullHD,Windows7Professional
软件:Holostudio2.3图像和细胞分析软件

系统性能
工作距离:1.2mm
数字聚焦范围:最大50×景深
抓拍时间:低于4μs/图像
立体取样:1024×1024pixels(全息图)
捕获速度:4fps(1024×1024像素)
重建速度:4fps(512×512像素),1fps(1024×1024像素)
样品照度:~0.1mW/cm
样品容器高度:最大45mm
电压:100-249VAC-47/63Hz功率(w/oPC):max.80W
体积(L×W×H):250×160×180mm重量:2.5kg

自2008年起，瑞典Phiab公司的激光全息分析系统就有大量用户文献发表，特挑选近三年文献与大家分享。

2014

Live-cell mass profi领: an emerging approach in quantitative biophysicsNature Methods

The role of pi3k/akt-related pip5k1α and the discovery of its selective inhibitor for treatment of advanced prostate cancerPNAS

Quantitative stain-free and continuous multimodal monitoring of wound hea领 in vitro with digital holographic microscopyPLoS ONE

Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in l929 cellsPLoS ONE

Edaravone protects against methylglyoxal-induced barrier damage in human brain endothelial cellsPLoS ONE

Environmental and toxicological impacts of glyphosate with its formulating adjuvantInternational Journal of Agricultural, Biosystems Science and Engineering

Prevention and treatment of colon cancer by peroral administration of hamletGut

2015

From immobilized cells to motile cells on a bed-of-nails: effects of vertical nanowire array density on cell behaviorScientific Reports 2015

Enhanced cytotoxicity of folic acid-targeted liposomes co-loaded with c6 ceramide and doxorubicin: in vitro evaluation on hela, a2780-adr and h69-arMolecular Pharmaceutics

Targetedtumor therapy byrubia tinctorum l.: analytical characterization ofhydroxyanthraquinones andinvestigation oftheir selectiveCancer Cell International

Cathelicidins positively regulate pancreatic β-cell functionsThe FASEB Journal（江南大学发表）

Macrophage polarization and the effect of microrna-155 administered in water-in-oil-in-water multiple emulsion formulationsJournal of Clinical & Cellular Immunology

Oloxamer bioadhesive hydrogel for buccal drug delivery: cytotoxicity and trans-epithelial permeability evaluations using tr146 human buccalInternational Journal of Pharmaceutics

Incubator proof miniaturized holomonitor to in situ monitor cancer cells exposed to green tea polyphenol and preosteoblast cells adheringJ. Biomed. Opt

Reversal of chemoresistance in ovarian cancer by co-delivery of mdr inhibitors and paclitaxel using a targeted liposomal platformThe FASEB Journal

Induction of morphological changes in death-induced cancer cells monitored by holographic microscopyJournal of Structural Biology

2016

Shielding of the geomagnetic field alters actin assembly and inhibits cell motility in human neuroblastoma cellsScientific Reports (中科院生物物理所发表）

Pseudomonas aeruginosa n-3-oxo-dodecanoyl-homoserine lactone elicits changes in cell volume, morphology, and aqp9 characteristics inFrontiers in Cellular and Infection Microbiology

The curcumin analog c-150, influencing nf-κb, upr and akt/notch pathways has potent anticancer activityin vitroandin vivoPLOS ONE