Santa Cruz Immunoprecipitation投诉表
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SantaCruz,SantaCruz代理,SantaCruz北京代理,SantaCruz上海,SantaCruz国内代理热烈祝贺上海起发获得SantaCruzZG区代理2013年9月开始正式签约SantaCruz,所有产品售后均有上海SantaCruz负责,1**%负责,并在上海备有20000多种现货抗体,价格优惠,欢迎广大经销商咨询178406/news_328698.html上海起福生物科技有限公司SantaCruz专业代理,具体产品信息欢迎电询:4006551678TECHNICALSERVICEGUIDE:IMMUNOPRECIPITATIONCatalog#:Lot#:A.ProblemandPreviousExperience1.Whatisthespecificproblemyouareexperiencing?2.Didthissamevialofproductworkinthepast?Whatweretheresults?3.Didotherlotsofthisproductworkinthepast?Whichlots?Whatweretheresults?B.Samples1.Fromwhichspecies(animal)wasthesampleandwhattypewasused(celllysate,tissue,purifiedprotein,etc.)?Whatlysisbufferwasused?2.Howmanycellswerelysedorwhatwastheproteinconcentrationandvolumeofthesamplethatwasusedintheimmunoprecipitation?C.Pre-Clearing1.Wasapre-clearstepdonepriortoaddingprimaryantibody?2.Whatspeciesofimmunoglobulin(rabbit,goat,etc.)wasusedforthepre-clearstep?3.Whatproteinagarose-conjugatewasusedinthepre-clearstep(Protein-A,Protein-GAgarose,etc.)?D.AntibodiesandImmunoprecipitationReagents1.Howmanymicrogramsofprimaryantibodywereaddedtothetotalcelllysate?2.Wastheprimaryantibodydirectlyconjugatedtoagarose?3.Howlongwastheprimaryincubatedwiththetotalcelllysate?4.Whatimmunoprecipitationreagentwasused(Protein-AAgarose,Protein-GAgarose,etc.)?5.Atwhattemperaturehastheprimaryantibodybeenstored?Hasiteverbeenfrozen?E.WashesandSpins1.Isthefinalwashdonewithastringentreagent(RIPA)oramildreagent(PBS)?2.Howfastwerethesamplesspun(inRPM)?上海起福生物科技有限公司联系人:杨建辉400电话:4006551678办公电话:021-50724187传真:021-50724961*8006手机:15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址:上海市浦东新区绣川路561号1102室
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