Santa Cruz Immunohistochemistry投诉表
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SantaCruz产品投诉指南关于SantaCruz产品投诉流程,请参照附件PDF文档,在您填表前,务必认真
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SantaCruzIF投诉表-2014
SantaCruz,SantaCruz代理,SantaCruz北京代理,SantaCruz上海,SantaCruz国内代理热烈祝贺上海起发获得SantaCruzZG区代理2013年9月开始正式签约SantaCruz,所有产品售后均有上海SantaCruz负责,1**%负责,并在上海备有20000多种现货抗体,价格优惠,欢迎广大经销商咨询178406/news_328698.html上海起福生物科技有限公司SantaCruz专业代理,具体产品信息欢迎电询:4006551678TECHNICALSERVICEGUIDE:ImmunohistochemistryCatalog#:Lot#:PROBLEMANDPREVIOUSEXPERIENCE1.Whatisthespecificproblemyouareexperiencing?2.Didthissamevialofproductworkinthepast?Whatweretheresults?3.Didotherlotsofthisproductworkinthepast?Whichlots?Whatweretheresults?STAININGPATTERN1.Whatpatternofstainingwasexpectedandwhatwasobserved?CONTROLS1.Hastheprimaryantibody(usedintheimmunohistochemistry)beenusedsuccessfullyinanyotherapplications(WesternBlot,Immunoprecipitation,Immunohistochemistrywithdifferentmethodoffixation)withthesamesample?2.Whatpositiveand/ornegativecontrolsweredoneandwhatweretheresults?SAMPLESANDFIXATION1.Fromwhichspecies(animal)wasthesample?2.Whattypeofsamplewasstained(frozentissue,paraffin-embeddedtissue,tissueculturecells)?3.Whatmethodoffixationwasused(acetone,formalin,methanol)?4.WasAntigen-Retrievalperformed?Ifso,bywhatmethod(heattreatment,enzymedigestion)?BLOCKING1.Whatweretheblockingconditions(blockingsolution,speciesofserum,lengthofincubation)?PRIMARYANTIBODY1.Atwhatdilution(s)wastheprimaryantibodyusedandwhatwasthediluent?2.Whatweretheincubationconditions(time,temperature)fortheprimaryantibody?3.Atwhattemperaturehastheprimaryantibodybeenstored?Hasiteverbeenfrozen?SECONDARYANTIBODY1.Fromwhichcompanywasthesecondaryantibodyobtainedandwhattypeofsecondarywasbeingused(goatanti-rabbit,rabbitanti-mouse,etc.)andwhatsecondaryantibodyconjugatewasused(biotin,FITC,Rhodamine,TexasRed)?2.Atwhatdilution(s)wasthesecondaryantibodyusedandwhatwasthediluent?上海起福生物科技有限公司联系人:杨建辉400电话:4006551678办公电话:021-50724187传真:021-50724961*8006手机:15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址:上海市浦东新区绣川路561号1102室
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