继续求助免疫荧光检测NF

情调一丁点 2017-01-05 00:23:41 416 浏览

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继续求翻译: Ethylacetate(Quimis，p.a.)，methanol(Quimis，p.a.)，sec-butanol(Merck，HPLCgrade)，oleicacid(Merck，extrapure)，hydrogenperoxide(Interox，70%，w/waqueoussolution)，aluminumoxideforc... Ethyl acetate (Quimis， p.a.)， methanol (Quimis， p.a.)， sec- butanol (Merck， HPLC grade)， oleic acid (Merck， extra pure)， hydrogen peroxide (Interox， 70%， w/w aqueous solution)， aluminum oxide for chromatography (Fluka， 0.05–0.15 mm)， aluminum oxide for column chromatography (Acros Organics， activated， acidic， 0.1–0.5 mm)， aluminum tri-sec-butoxide (Aldrich， 99.99%)， oxalic acid (Synth， p.a.)， di-n-butyl ether (Fluka， >99%) and soybean oil (Cargill) were used as received. The solution of anhydrous hydrogen peroxide (24%， w/w) in ethyl acetate was prepared from aqueous H2O2 (70%， w/w) in ethyl acetate by azeotropic distillation with a Dean-Stark apparatus [14] The products were identified by gas chromatography–mass spectroscopy (GC–MS) using a Hewlett-Packard 5890 gas chromatograph equipped with a AT-Wax column and interfaced with a Hewlett-Packard 5971A mass spectrometer. Helium was used as the carrier gas. A similarly equipped Hewlett-Packard5890 gas chromatograph using nitrogen as the carrier gas and a flame ionization detector was used for product quantification， with the help of calibration curves. 展开