请问你能找到关于蛋白质纯化与分离的英文文献及翻译么

斯不说话 2015-01-04 20:46:08 527 浏览

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vg枯产喏 2015-01-05 00:00:00

首先要确认具体哪个蛋白质的文章而且不会有那篇文章仅仅和纯化分离有关的，一般总是要包括克隆构建，发酵表达，分离纯化，检测验证活性。有的还会包括功能的研究，或者结晶的研究等等能找到这样的文章并不难，翻译是要自己去看的，多看看问题并不大

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请问你能找到关于蛋白质纯化与分离的英文文献及翻译么:

求翻译英文文献: 2.2. Material Characterizations. A field emission scanning electron microscope (SEM， JEOL 6701F) was used to investigate the morphologies， particle sizes of the samples. Transmission electron microscopy and elemental compositions of the ... 2.2. Material Characterizations. A field emission scanning electron microscope (SEM， JEOL 6701F) was used to investigate the morphologies， particle sizes of the samples. Transmission electron microscopy and elemental compositions of the samples were determined by JEM-2100F (JEOL) coupled with an energy-dispersive X-ray spectroscopy (EDX， Phoenix) system. Wide-angle and low-angle X-ray powder diffraction (XRD) of the as-obtained samples were recorded on a Rigaku D/max-2500 with Cu Kα radiation (λ = 1.540 56 Å) operated at 40 kV and 200 mA. Raman measurements were performed using a DXR from Thermo Scientific with a laser wavelength of 532 nm. To calculate the pore size distribution and pore volumes， the nitrogen absorption and desorption isotherms were measured at 77.3 K with an Autosorb-1 specific surface area analyzer from Quantachrome. The content of N in N-doped porous carbon was determined by NHC elemental analysis using Flash EA 1112. Thermogravimetric (TG) analysis of S/C composite was performed on TG/DTA 6300 in an N2 flow to obtain the S content in the composite. A four-contact method was applied to measure the powder electronic conductivity of porous carbons. The powder sample was pressed to disk at 4 MPa with two stainless-steel plungers， whose resistance was measured by a Keithley 2400 digital multimeter in fourwire mode. The conductivity of the sample was calculated according to the resistance and the size of the disk. Information of the surface elements was obtained by X-ray photoelectron spectroscopy (XPS) performed on the Thermo Scientific ESCALab 250Xi using 200 W monochromatic Al Kα radiation. The 500 μm X-ray spot was used for XPS analysis. The base pressure in the analysis chamber was about 3 × 10−10 mbar. All reported data of XPS binding energy are calibrated based on the hydrocarbon C 1s line at 284.8 eV from adventitious carbon. Spectra were fitted with Lorentzian−Gaussian functions and smart background using Thermo Avantage software. 展开

求助，英文文献翻译！: 2. Experimental 2.1 Materials Nano-CaCO3 particles (D50: 30−70 nm by TEM (Fig. 1) and BET: ca.17m2·g-1) were supplied by Shanghai Zhuoyue Nanotech Corporation. Methyl methacrylate (MMA， Shanghai Lingfeng Chemicals) was purified ... 2. Experimental 2.1 Materials Nano-CaCO3 particles (D50: 30−70 nm by TEM (Fig. 1) and BET: ca.17m2·g-1) were supplied by Shanghai Zhuoyue Nanotech Corporation. Methyl methacrylate (MMA， Shanghai Lingfeng Chemicals) was purified by distillation under reduced pressure; potassium persulphate (K2S2O8， initiator， Shanghai Lingfeng Chemicals) was of chemical grade. Silane coupling agent A174 (γ-methacryloxypropyltrimethoxysilane) was purchased from Shanghai Yaohua Factory. Polyvinylchloride (PVC， WS-1000S) was supplied by Shanghai Chlor-Alkali Chemical Co.， Ltd. Fig. 1 Morphology of untreated CaCO3 nanoparticles. Fig. 2 Morphology of PMMA-coated CaCO3 nanoparticles. 2.2 PMMA emulsion polymerization on CaCO3 nanoparticles 2.2.1 Surface silanation of nano-CaCO3 particles The nano-CaCO3 particles were homogeneously dispersed in ethanol (solid content 20%) by a sonication dispersion equipment. The slurry was then heated to 80°C with stirring， and the silane coupling agent A174 (5%， calculated based on the weight of nano-CaCO3 particles) was added into the slurry. After the slurry was stirred for 120 min at 80°C， it was filtered and the filter cake was then dried at 120°C in low vacuum for 120 min to obtain silanated CaCO3 powders. 展开

求助，英文文献翻译~: PMMA has good compatibility with PVC due to specific interaction of a hydrogen bonding type between carbonyl groups (C=O) of PMMA and hydrogen from (CHCl) groups of PVC (Belhaneche-Bensemra et al.， 2002; Ramesh et al.， 2002). Zhou et al... PMMA has good compatibility with PVC due to specific interaction of a hydrogen bonding type between carbonyl groups (C=O) of PMMA and hydrogen from (CHCl) groups of PVC (Belhaneche-Bensemra et al.， 2002; Ramesh et al.， 2002). Zhou et al. (2001) studied the relationship between the coating thickness of PMMA on the surface of talc and mechanical properties of PMMA-g-talc/PVC composites， and found that there existed a critical thickness. Xie (2001) found that the PMMA coating on talc improved the dispersion of talc in the PVC matrix and enhanced the interfacial adhesion between talc and PVC. There appears to be a critical coating thickness of PMMA on the talc surface for optimum toughening. Quan et al. (2002) reported improved toughness and intension of the PVC composites filled with CaCO3/ACR core-shell complex particles prepared by in-situ emulsion polymerization of acrylic ester. In the present work， PMMA coated on the surface of CaCO3 nanoparticles by in-situ emulsion polymerization was characterized by FT-IR and 1H-NMR. The effects of PMMA thickness and particle fraction on the mechanical properties of PMMA-coated CaCO3 /PVC nanocomposites were also investigated. 展开

蛋白质分离纯化英文版本介绍（详细）:

纳米材料英文文献加翻译:

通信方面的英文文献翻译！！: 以下是文献的一部分，愿意帮忙的高手留下邮箱，我把文件发给你，若能完成，加分：SpatialCompressiveSensingApproachForFieldDirectionalityEstimation.I.INTRODUCTIONVarietyoft... 以下是文献的一部分，愿意帮忙的高手留下邮箱，我把文件发给你，若能完成，加分： Spatial Compressive Sensing Approach For Field Directionality Estimation. I. INTRODUCTION Variety of techniques for field directionality estimation were studied in literature [1]-[5]. Thus， a theoretical analysis of the relationship between the hydrophone array output and the noise field was conducted in [1]-[5]. The developed techniques were based on the array beamformer output or the crossspectral matrix between outputs of array elements [4]-[5]. The problem of a field directionality estimation in ocean， using horizontal line towed array was also addressed in literature [5]- [8]. Recently， problems of direction of arrival and field directionality estimation for moving sensors arrays have attracted renewed interest [9]-[12]. It was shown that an array motion can improve an array performance assuming temporal coherence of successive samples [10]-[11]. In [12]， the wavefield sampling method that exploits the linear relationship between the noise field and the collection of beamformer outputs over various array orientations was proposed. It was shown that the wavefield sampling (WS) method outperforms other tested methods. This algorithm was implemented via the recursive estimation method and its convergence to the unique solution was promised for a specific set of array orientations and beamformer look directions. However， a method for a proper array orientation and beamformer look direction sequence selection remains an open question. The quality of the field directionality estimation is determined by the angular resolution. The higher angular resolution is， the more accurate estimation of the far field sources， and better detection performance can be achieved. One of fundamental relations in the array signal processing is that the angular resolution is directly proportional to the number of the array elements [13]. This relation motivates the desire for longer arrays that can achieve higher resolution. Unfortunately， the requirement contradicts the implementation and installation limitations that motivate shorter arrays. Moreover， implementation of longer arrays for maneuvering platforms such as unmanned underwater vehicles (UUV) can even be impossible [14]. These contradictions motivate the quest for alternative array signal processing methods. Usually， the field directionality is modeled as a finite set of strong far-field narrow-band sources and an isotropic lowpower noise [1]. In this work， the model of the field directionality is adopted in the following way. First， the bearing angle space is uniformly sampled into a large number of discrete angles. Next， it is assumed that ether the high energy that corresponds to the far-field strong sources or the low-energy that corresponds to the isotropic noise is received at the sensor array from every of these discrete azimuth angles. 展开

求一篇关于自动洗瓶机的英文文献及中文翻译: 求一篇关于自动洗瓶机的英文文献及中文翻译，中文翻译字数在5000字左右，看过后给分，以免使胡乱翻译的。急求啊~~~~~~~~~~~~~~有意者可以发送至邮箱jay55111@sina.com如果有人有的话，... 求一篇关于自动洗瓶机的英文文献及中文翻译，中文翻译字数在5000字左右，看过后给分，以免使胡乱翻译的。急求啊~~~~~~~~~~~~~~有意者可以发送至邮箱jay55111@sina.com 如果有人有的话，再加100分，有QQ为证，416975023，今晚12点前截止展开

如何对分离纯化后的蛋白质进行种类及量的分离:

蛋白质分离纯化常用方法:

求蛋白质分离纯化论文: 题目：生物大分子分离纯化方法----蛋白质技术要求：将生物大分子（蛋白质）分离纯化常见方法做简单介绍，包括各方法的优缺点等，同时着重介绍常见凝胶色谱应用等方面一些新的方法。 Z好是一篇完整的论文

蛋白质分离纯化的四种方法: 论述题：请说出可用于蛋白质分离纯化的四种方法及原理？很急，望帮忙，谢谢！！

蛋白质分离纯化的产品特点:

蛋白质药物的分离纯化方法: 蛋白质药物的分离纯化方法

蛋白质纯化相关为文献在那里查找: Z好的PDF文档

谁能帮我翻译一下这段英文文献啊: Fig. 1 shows that diamond particles are dispersed in the copper matrix. Since the diamond particles are easy to be stripped off during mechanical polishing， small pits are left on the surface of samples. When the samples were analyzed b... Fig. 1 shows that diamond particles are dispersed in the copper matrix. Since the diamond particles are easy to be stripped off during mechanical polishing， small pits are left on the surface of samples. When the samples were analyzed by electron probe for carbon， the particles displayed high carbon peaks， as shown in Fig. 2. Fig. 3 exhibits the analyzed result for extracted product of the copper–diamond composite by X-ray diffraction (XRD) analysis. It is shown that three dif- fraction peaks exist， shown as A， B and C， respectively. The interplanar distances corresponding to the three peaks and standard ones for diamond are compared in Table 1. The measured values of the interplanar distances are in good agreement with the standard ones， demon- strating that the extracted product should be the carbon in diamond state. Fig. 4(a) gives a TEM micrograph showing distribution and morphology of diamond particles (indicated as A， B， and C) in the copper–diamond composite. The calibration of interplanar distance for diffraction rings in Fig. 4(b) was given in Table 2. Compared to Table 1， it is also demon- strated that the calibrated interplanar distances for the diffraction rings conform to the standard ones of diamond. Therefore， it is believed that the graphitization of diamond particles hardly occurs after sintering at 1150–1220 K in the copper–diamond composite. Although diamond is a metastable allotropic modifica- tion of carbon， the graphitization of diamond particles in the copper–diamond composite did not occur， or the degree of graphitization was too small to be found. It is believed that the beginning temperature of graphi- tization would be associated with the purity of diamond powders. The impurities such as some metallic elements reduce the beginning temperature of diamond graphitiza- tion. When diamond is fabricated by the static synthesis method， Fe and Ni are often used as catalysts and are present in diamond as impurities to decrease the beginning temperature of graphitization [6]. It is understandable that both for the graphitization and its reverse reactions， their activation energies can be decreased by catalytic agents. Compared with the diamond fabricated by static synthesis， the diamond powders prepared by explosion method do not contain metallic impurities， thus leading to an obvious increase in the beginning temperature of graphitization. Hence， the detonation synthetic diamond might be more resistant to graphitization and probably suitable for the electric contact materials. 展开

帮忙翻译一段关于方酸染料合成的的英文文献急急急: Synthesisofsquarainedyeswasreportedatleastin1966。Theyarederivedfromsquaricacidwhichundergoesanelectrophilicaromaticsubstitutionreactionwithananilineoranotherelectronrich... Synthesis of squaraine dyes was reported at least in 1966 。They are derived from squaric acid which undergoes an electrophilic aromatic substitution reaction with an aniline or another electron rich derivative to form a highly conjugated product with extensive charge distribution. For instance， squaraine dyes are also formed via reaction of squaric acid or its derivatives with so-called "methylene bases" like 2-methyl-indolenines， 2-metyl-benzthiazoles or 2-methyl-benzo-selenazoles. Indolenine-based squaraines combine good photostability including high quantum yields when bound to proteins and reactive versions of these dyes are commonly used as fluorescent probes and labels for biomedical applications . 展开

请说出可用于蛋白质分离纯化的四种方法及原理？？？: 请说出可用于蛋白质分离纯化的四种方法及原理？？？

求达人翻译下英文文献关于光系统Ⅱ和除草剂通顺Z重要: PhotosystemIIisthemulti-enzymaticchlorophyll-proteincomplex(water-plastoquinoneoxido-reductase)locatedinthethylakoidmembraneofalgae，cyanobacteriaandhigherplants.Itisanint... Photosystem II is the multi-enzymatic chlorophyll-protein complex (water-plastoquinone oxido-reductase) located in the thylakoid membrane of algae， cyanobacteria and higher plants. It is an integral part of the electron transport chain that catalyses primary charge separation. This protein complex consists of over 25 polypeptides， which make up a light-harvesting chlorophyll protein complex (LHCII)， a reaction centre and the water-splitting system， also called the oxygen evolving complex (OEC). The PSII complex also contains the target site of the most widely used photosynthetic herbicides."^ The preparation of a biosensor for the detection of polluting compounds is based on the specific characteristics of PSII. Under illumination PSII drives electron transfer which is inhibited by specific chemical compounds (e.g.， herbicides). Thus， the photosynthetic membrane isolated firom higher plants and photosynthetic micro-organisms， immobilised and stabilised， will serve as the biosensor biomediator. The effect of compounds that alter or inhibit photosynthetic activity， measured as oxygen evolution， electron transport or fluorescence can then be translated and monitored by amperometric or optical systems. Recent experiments that were performed by our this team resulted in amperometric， potentiometric， optical biosensors for herbicides based on isolated and immobilised PSII particles exhibiting stable biological material and a highly sensitive monitoring response (limit of detection in the nanomolar range and for diuron herbicide in the picomolar range).^'^ However， the system is specific to photosynthetic herbicides but not very selective since several classes of compounds (herbicides represented by triazines， ureas， diazines， phenols) can bind to proteins of the PSII complex， most of them to the Dl protein of the reaction centre. 展开

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