DLD 新产品EA611/96 说明书

本文由 上海起发实验试剂有限公司 整理汇编

2018-09-29 10:02 596阅读次数

收藏 评价 举报

• 分享

立即下载

参与评论

评论

登录后参与评论

登录或新用户注册

• 微信登录
• 密码登录
• 短信登录

请用手机微信扫描下方二维码
快速登录或注册新账号

微信扫码，手机电脑联动

注册登录即表示同意《仪器网服务条款》和《隐私协议》

账  号：

密  码：

图片码： 看不清？
换一张？

手机和邮箱号注册新账号>> 忘记密码？

手机号：

图片码： 看不清？
换一张？

短信码： 获取短信码

手机和邮箱号注册新账号>> 忘记密码？

更多资料

• DLD 新产品EA611/96 说明书

  世界ding级实验材料供应商DLDDiagnostikaGmbH正式授权上海起发为其ZG代理，DLDDiagnostikaGmbH在一直是行业的标杆,一直为广大科研客户提供Z为优质的产品和服务,上海起发一直秉承为ZG科研客户带来**的产品，**的服务，签约DLDDiagnostikaGmbH就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们Zda的收获DLDDiagnostikaGmbHZG代理,DLDDiagnostikaGmbH上海代理，DLDDiagnostikaGmbH北京代理，DLDDiagnostikaGmbH广东代理，DLDDiagnostikaGmbH江苏代理DLDDiagnostikaGmbH湖北代理,DLDDiagnostikaGmbH天津,DLDDiagnostikaGmbH黑龙江代理,DLDDiagnostikaGmbH内蒙古代理,DLDDiagnostikaGmbH吉林代理,DLDDiagnostikaGmbH福建代理,DLDDiagnostikaGmbH江苏代理,DLDDiagnostikaGmbH浙江代理,DLDDiagnostikaGmbH四川代理,DLD开发，销售和提供科学服务，用于体外诊断的高质量创新测试。产品范围包括自身免疫性疾病，心血管疾病，癌症疾病和肾功能不全等所谓的“利基测试”，DLD是研究与常规实验室之间的协调点。DLDdevelops,marketsandprovidesscientificserviceforhighqualityinnovativetestsforin-vitro-diagnosis.Theproductsrangeincludesso-callednichetests"forautoimmunediseases,cardiovasculardiseases,cancerdiseasesandrenaldysfunction.DLDisthecoordinatingpointbetweenresearchandroutinelab.Calretinin-ELISACatalog-No.EA611/96FormatMicrotiterplate(12x8determinations,singlebreakapartwells;coatedwithpolyclonalcalretininantibodiesProcedureSamplevolume:15碌lpatientserumorplasma1.SamplePreparation:60minshakingatroomtemperature2.ELISA:5hoursincubationatroomtemperatureMeasurementinELISAreaderat450nm(620nmreferencewavelength)6Standardswithrange:0.25ng/ml-4.0ng/ml2Kitcontrols,lyophilizedWashbuffer50xconcentratedAllotherreagentsreadyforuseClinicalFieldsOncology,cancerscreening,occupationaldiseasesDiseasesMesotheliomaSensitivity0.05ng/mlCharacteristicsSandwichELISAwithmicrotiterplateformateHighlyspecificandsensitiverabbitanti-calretininantibodyGoodlinearitybetween0.25-4.0ng/mlEasytohandle,totalassaytimeis6hoursSuitablefortestinglargeseriesofserumorplasmasamplesValidatedinlargercase-controlstudiesInvolvedinprospectivestudies部分产品2017Zxin报价如下:货号品名规格目录价品牌EA110/96ACHRABAssayELISA12x811975dld-diagnostikaEA611/96CalretininELISA12x89879dld-diagnostikaEA112/9621-HydroxylaseAntibodyELISA12x811975dld-diagnostikaEA300/9625-OHVitaminDELISA12x85888dld-diagnostikaEA601/48TitinAntibodyELISA6x84191dld-diagnostikaEA111/96Aquaporin-4(AQP4)AntibodyELISA12x813871dld-diagnostikaEA201/96ADMAELISA12x89879dld-diagnostikaEA212/96ADMAFastELISA12x89879dld-diagnostikaEA209/96ADMAHighSensitiveELISA12x811975dld-diagnostikaEA207/192ADMAArginineELISA24x813871dld-diagnostikaEA205/96HomoarginineELISA12x813871dld-diagnostikaEA203/96SDMA-ELISA12x89879dld-diagnostikaEA101/96TSHReceptorAntibodyELISA12x84590dld-diagnostikaEA104/96GAD6ntibodyELISA12x86985dld-diagnostikaEA114/96IA2AntibodyELISA12x86985dld-diagnostikaEA102/96ZincTransporter8(ZnT8)AntibodyELISA12x89879dld-diagnostikaEA602/96SerotoninELISA12x83992dld-diagnostikaEA630/96SerotoninHighSensitiveELISA12x84191dld-diagnostikaEA614/192Nor-/MetanephrineinUrineELISA2x964890dld-diagnostikaEA612/192Nor-/MetanephrineinPlasmaELISA2x965089dld-diagnostikaEA604/96AdrenalineELISA12x84590dld-diagnostikaEA610/96NoradrenalineELISA12x84590dld-diagnostikaEA608/96DopamineELISA12x84590dld-diagnostikaEA631/192Noradrenaline/DopamineHighSensitiveELISA2x969879dld-diagnostikaEA632/96AdrenalineHighSensitiveELISA12x87883dld-diagnostikaEA633/96NoradrenalineHighSensitiveELISA12x87883dld-diagnostikaEA634/96DopamineHighSensitiveELISA12x87883dld-diagnostikaEA613/192BI-CATELISA(Adrenaline,Noradrenaline)inUrine&Plasma2x965688dld-diagnostikaEA603/288CATELISA(Adrenaline,Noradrenaline,Dopamine)inUrine&Plasma3x966586dld-diagnostikaEA213/96HistamineELISA12x83892dld-diagnostikaEA208/961-MethylhistamineELISA12x84690dld-diagnostika我们公司Zda优势是强大的采购，1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，2：货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，3：提供加急服务，一般1-2周到货，超过时限加急费全免4：价格公道，绝大部分价格有优势，当然不能保证1**%产品都是价格Zdi,因为价格Zdi意味着没有服务.5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大交大复旦，中山等100多所大学，ROCHE，阿斯利康，国药，fisher等500多家公司6：我们还是Santa,AdvancedBiotechnologiesInc,DLDDiagnostikaGmbH,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.Dako,Inc,GePromegaridege,DLDDiagnostikaGmbHBiotechnologyGmbH;iduron,InnovativeResearchofAmerica,Ludger,neuroprobe,DLDDiagnostikaGmbH,Polysciences,prospecbi,QA-BIO,quickzyme,FULLERLABS,INC,sterlitech；sysy,TriLinkBioTechnologies,Inc；worthington-biochem,zyagen等几十家国外公司授权代理。7：我们还是invitrogen,qiagen,MidlandBioProductsCorporationam,sigma;neb,roche,merck,rnd,BD,GE,pierce,BioLegend等知名批发，欢迎合作。[详细]

  2018-09-29 10:02

  产品样册

  |

  • 
  • 
  • 
  • 

• DLD Diagnostika 2017价格表

  世界ding级实验材料供应商BioCheck正式授权上海起发为其ZG代理，BioCheck在一直是行业的标杆,一直为广大科研客户提供Z为优质的产品和服务,上海起发一直秉承为ZG科研客户带来**的产品，**的服务，签约BioCheck就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们Zda的收获BioCheckZG代理,BioCheck上海代理，BioCheck北京代理，BioCheck广东代理，BioCheck江苏代理BioCheck湖北代理,BioCheck天津,BioCheck黑龙江代理,BioCheck内蒙古代理,BioCheck吉林代理,BioCheck福建代理,BioCheck江苏代理,BioCheck浙江代理,BioCheck四川代理BIOCHECK公司由创始人Dr.JohnChen,Medix创立,是一家抓也提供肿瘤标志物，心肌标志物，激素类Z高性价比的抗体的公司。简要原理利用竞争酶联免疫方法，预先在微孔中包被羊抗兔抗体，实验时先后加入氯霉素标准品或待测样本，氯霉素酶标抗原和兔抗氯霉素抗体。经过室温温育，反应液中的兔抗氯霉素抗体与微孔板上的羊抗兔抗体结合，待测样品中的抗原与氯霉素酶标抗原竞争微孔板上的兔抗氯霉素抗体。洗涤后，没有与抗体结合的待测样品中的抗原或酶标抗原被洗去，再加入反应底物，结合的酶标抗原的酶将底物转化为蓝色产物，加入终止液后颜色由蓝色变为黄色。反应完成后，样品中氯霉素含量越多，反应呈色就越浅；反之，样品中氯霉素含量越少，则呈色越深。利用标准曲线可计算出样品中氯霉素含量。技术参数检测蜂蜜、蛋类、牛奶、奶粉、水产品、动物组织(肌肉、肝脏等)、饲料、血清、血浆及尿液样本中存在的氯霉素，定量限可达0.025ppb。No.样品检测下限1蜂蜜0.02ppb(0.02ng/g)2蛋类0.02ppb(0.02ng/g)3牛奶0.002ppb(0.002ng/ml)4奶粉0.012ppb(0.012ng/g)5虾，鱼及肉类0.08ppb(0.08ng/g)6饲料0.08ppb(0.08ng/g)7血清/血浆0.02ppb(0.02ng/ml)8尿液0.04ppb(0.04ng/ml)交叉反应名称百分比氯霉素碱0.4%甲基氯霉素<0.04%回收率No.样品回收率1蜂蜜70%~110%2牛奶90%~130%3蛋，虾，鱼及肉类95%~120%4饲料95%~120%5血清/血浆90%~120%6尿液1**%~130%PD-1ENZYMEIMMUNOASSAYTESTKITCatalogNumber:BC-1301EnzymeImmunoassayfortheQuantitativeDeterminationofPD-1ConcentrationinHumanSerumandPlasmaFORRESEARCHUSEONLYNotforuseindiagnosticproceduresINTRODUCTIONProgrammedcelldeathprotein1,(PD-1,CD279,PDCD1),isacellsurfacereceptorthatiscriticalfortheregulationofTcellinflammatoryactivityandmaintainsperipheraltoleranceintheimmunesystem(1).WithanextracellularIgVdomain,atransmembranedomain,andanintracellulartailwithtwophosphorylationsites(SHP-1andSHP-2),PD-1isabletointeractwithligandsPD-L1andPD-L2todown-regulatetheimmunesystemandsuppressTcellactivity(2).Uponantigenrecognition,anactivatedTcellexpressesPD-1onitssurfaceandproducesinterferons,whichinducesexpressionofPD-L1,therebypromotingapoptosisorcelldeath.(3)Howeverwhenhijackedbytumorcells,aberrantexpressionofPD-1ligandsinhibitsimmune-modulatoryTcellactivation,leadingtodiseaseprogression(4).ElevatedlevelsofPD-1inserumandplasmahavebeenassociatedwithrheumatoidarthritisandskinsclerosis(5,6).Also,PD-1ispredominantlyexpressedbytumorinfiltratingTcells(7).FurtherresearchimpliesthatusingmonoclonalantibodiestotargetthePD-1immunologiccheckpointhascontributedtobreakthroughprogressinunderstandingandtreatingcancerssuchasmelanomaandothervariousnon-smallcelllungcancer(4).PRINCIPLEOFTHEASSAYThePD-1ELISAisbasedontheprincipleofasolidphaseenzyme-linkedimmunosorbentassay.TheassaysystemutilizesauniquemonoclonalantibodypairdirectedagainstadistinctantigenicdeterminantonthePD-1molecule.Onemousemonoclonalanti-PD-1antibodyisusedforsolidphaseimmobilization(onthemicrotiterwells).Anothermousemonoclonalanti-PD-1antibodyconjugatedtohorseradishperoxidase(HRP)isintheenzymeconjugatesolution.Thetestsamplesareallowedtoreactsequentiallywiththetwoantibodies,resultinginthePD-1moleculestobesandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Aftertwoseparate60-minuteincubationstepsatroomtemperature,thewellsarerinsedwithWashBuffertoremoveunboundlabeledantibodies.TMBReagentisaddedandincubatedfor30minutesunderdarkconditions,resultinginthedevelopmentofabluecolor.ThecolordevelopmentisstoppedwiththeadditionofStopSolution,changingthecolortoyellow.TheconcentrationofPD-1isdirectlyproportionaltothecolorintensityofthetestsamples.Absorbanceismeasuredspectrophotometricallyat450nm.REAGENTSANDMATERIALSPROVIDEDAntibody-CoatedWells(1plate,96wells)Microtiterwellscoatedwithmousemonoclonalanti-PD-150ng/mlPD-1Standard(0.5mL/vial)50ng/mLPD-1inphosphatebuffer-BSAsolutionwithpreservatives3.StandardandSampleDiluent(30mL/bottle,1bottle)Containsphosphatebuffer-BSAsolutionwithpreservativesEnzymeConjugateReagent(12mL/vial,1vial)Containsmousemonoclonalanti-PD-1conjugatedtohorseradishperoxidase20XWashBuffer(50mL/bottle,1bottle)PhosphatebufferwithdetergentsTMBReagent(11mL/bottle,1bottle)Containsone-stepTMBsolutionStopSolution(11mL/bottle,1bottle)Containsdilutedhydrochloricacid(1NHCl)STORAGECONDITIONSStoretheunopenedkitat2-8°Cuponreceiptandwhenitisnotinuse,untiltheexpirationshownonthekitlabel.Refertothepackagelabelfortheexpirationdate.Keepmicrotiterplateinasealedbagwithdesiccanttominimizeexposuretodampair.REAGENTPREPARATIONAllreagents首ldbeallowedtoreachroomtemperature(18-25°C)beforeuse.Foreachtestrun,prepareafreshstandardset.Dilute50ng/mLto10ng/mLwithStandard/Samplediluent.Preparetwo-foldserialdilutionsofthe10ng/mLStandardwithStandard/SampleDiluent:10ng/mL:0.12mLof50ng/mL+0.48mLofStandard/SampleDiluent5ng/mL:0.25mLof10ng/mL+0.25mLofStandard/SampleDiluent2.5ng/mL:0.25mLof5ng/mL+0.25mLofStandard/SampleDiluent1.25ng/mL:0.25mLof2.5ng/ml+0.25mLofStandard/SampleDiluent0.625ng/mL:0.25mLof1.25ng/mL+0.25mLofStandard/SampleDiluent0.313ng/mL:0.25mLof0.625ng/mL+0.25mLofStandard/SampleDiluent0.156ng/mL:0.25mLof0.313ng/mL+0.25mLofStandardDiluent0ng/mL:0.25mLofStandardDiluentPatientsamplesneedtobediluted4-foldpriortouse.Prepareaseriesofsmalltubes(i.e.,1.5mLmicrocentrifugetubes)andmix60Lofserumwith180LStandard/SampleDiluent.WorkingWashBuffer:Preparationof1XWashBufferfrom20XStock.Add50mLof20XWashBufferStockto950mLofDIH2O.TheWorkingWashBufferisstableat2-8°Cfor30days.NOTE:Anycrystalsthatmaybepresentduetohighsaltconcentrationmustberedissolvedatroomtemperaturebeforemakingthedilution.ASSAYPROCEDUREPrepareStandards.SeeReagentPreparation.Dilutesamples1:4dilution.SeeReagentPreparation.Securethedesirednumberofcoatedwellsintheholder.Dispense100mLofPD-1standards,andDILUTEDspecimensintotheappropriatewells.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300mLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLofPD-1WorkingEnzymeConjugateReagentintoeachwell.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300uLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLTMBsolutionintoeachwell.Incubatefor30minutesatroomtemperature(18-25°C).Stopthereactionbyadding100mLofStopSolutionintoeachwell.Gentlymixfor30seconds.Itisimportanttomakesurethatallthebluecolorchangestoyellowcolorcompletely.Readabsorbanceat450nmwithamicrotiterwellreaderwithin15minutes.CALCULATIONOFRESULTSCalculatethemeanabsorbancevalue(OD450)foreachsetofreferencestandards,controlsandsamples.Constructastandardcurvebyplottingthemeanabsorbanceobtainedforeachreferencestandardagainstitsconcentrationinng/mlongraphpaper,withabsorbanceonthevertical(y)axisandconcentrationonthehorizontal(x)axis.ThecorrespondingconcentrationofPD-1(ng/mL)canbedeterminedfromthestandardcurveusingthemeanabsorbancevalueforeachsample.Dependingonexperienceand/ortheavailabilityofcomputercapability,othermethodsofdatareductionmaybeemployed.Theobtainedvaluesofthesamples首ldbemultipliedbythedilutionfactorof4toobtainPD-1resultsinng/ml.EXAMPLEOFSTANDARDCURVEResultsofatypicalstandardrunwithabsorbencyreadingsat450nmshownontheYaxisagainstPD-1concentrationsshownontheXaxis.NOTE:Thisstandardcurveisforthepurposeofillustrationonly,and首ldnotbeusedtocalculateunknowns.Eachlaboratorymustgenerateitsowndataandstandardcurveineachexperiment.PD-1Absorbance(ng/ml)(450nm)00.0890.1560.1380.3130.1900.6250.2841.250.4812.50.85151.581102.776nm32.5(4502Absorbance1.510.500246810PD-1Conc.(ng/ml)PERFORMANCECHARACTERISTICSSensitivityTheminimumdetectableconcentrationofthePD-1ELISAassayasmeasuredby2SDfromthemeanofazerostandardisestimatedtobe0.15ng/ml.PrecisionIntra-AssayPrecisionWithin-runprecisionwasdeterminedbyreplicatedeterminationsofthreedifferentsamplesinoneassay.Within-assayvariabilityisshownbelow:Sample123#Replicates242424MeanPD-1(ng/mL)8.413.222.9S.D.0.61.00.9C.V.(%)7.0%7.2%3.8%.2Inter-AssayPrecisionBetween-runprecisionwasdeterminedbyreplicatemeasurementsofthreedifferentsamplesoveraseriesofindividuallycalibratedassays.Between-assayvariabilityisshownbelow:Sample123#Replicates202020MeanPD-1(ng/mL)9.113.525.3S.D.0.81.10.9C.V.(%)9.2%7.93.7RecoveryandLinearityStudiesRecoverySampleswerespikedwithknownPD-1levelsandassayedinduplicate.Themeanrecoverywas105.3%.PAIREXPECTEDOBSERVED%RECOVERYNUMBER[PD-1][PD-1](ng/mL)(ng/mL)188.8110.0%212.513.1104.8%32525.3101.2%LinearityThreesampleswereseriallydilutedtodeterminelinearity.Themeanrecoverywas99.6%.#DilutionExpectedObservedConc.(ng/ml)Conc.(ng/ml)%Expected1.1:210.1114.8%1:48.88.8N/A1:87.888.6%Mean=101.7%2.1:214.5110.6%1:413.113.1N/A1:811.688.5%Mean=99.6%3.1:226.5104.7%1:425.325.3N/A1:823.090.0%Mean=97.4%REFERENCESFife,B.T.,&Pauken,K.E.(2011).TheroleofthePD-1pathwayinautoimmunityandperipheraltolerance.AnnalsoftheNewYorkAcademyofSciences,1217(1),45-59.Riella,L.V.,Paterson,A.M.,Sharpe,A.H.,&Chandraker,A.(2012).RoleofthePD-1PathwayintheImmuneResponse.AmericanJournalofTransplantation:OfficialJournaloftheAmericanSocietyofTransplantationandtheAmericanSocietyofTransplantSurgeons,12(10),25752587.Zak,Krzysztof&Kitel,Radosaw&Przetocka,Sara&Golik,Przemysaw&Guzik,Katarzyna&Musielak,Bogdan&Dm领,Alexander&Dubin,Grzegorz&Holak,Tad.(2015).StructureoftheComplexofHumanProgrammedDeath1,PD-1,andItsLigandPD-L1.Structure.23..10.1016/j.str.2015.09.010.ZoranGatalica,CarrieSnyder,ToddManey,AnatoleGhazalpour,DanielHolterman,NianqingXiao,PeggyOverberg,IngaRose,GargiD.Basu,SemirVranic,HenryT.Lynch,DanielD.VonHoffandOmidHamid.ProgrammedCellDeath1(PD-1)andItsLigand(PD-L1)inCommonCancersandTheirCorrelationwithMolecularCancerType.CancerEpidemiolBiomarkersPrev.December12014(23)(12)2965-2970.Greisen,S.,Rasmussen,T.,Stengaard-Pedersen,K.,Hetland,M.,Hrslev-Petersen,K.,Hvid,M.,&Deleuran,B.(2013).Increasedsolubleprogrammeddeath-1(sPD-1)isassociatedwithdiseaseactivityandradiographicprogressioninearlyrheumatoidarthritis.ScandinavianJournalofRheumatology,43(2),101-108.Yanaba,K.,Hayashi,M.,Yoshihara,Y.,&Nakagawa,H.(2016).Serumlevelsofsolubleprogrammeddeath-1andprogrammeddeathligand-1insystemicsclerosis:Associationwithextentofskinsclerosis.TheJournalofDermatology,43(8),954-957.Ahmadzadeh,M.,Johnson,L.A.,Heemskerk,B.,Wunderlich,J.R.,Dudley,M.E.,White,D.E.,&Rosenberg,S.A.(2009).TumorantigenspecificCD8TcellsinfiltratingthetumorexpresshighlevelsofPD-1andarefunctionallyimpaired.Blood,114(8),15371544.部分产品Zxin报价如下：货号品名规格价格品牌BC-1009AFPEIAKitkit3980BioCheckBC-1011CEAEIAKitkit4016BioCheckBC-1013CA125EIAKitkit4970BioCheckBC-1015CA15-3EIAKitkit4970BioCheckBC-1017CA19-9EIAKitkit4970BioCheckBC-1019PSAEIAKit*kit3962BioCheckBC-1021FreePSAEIAKit*kit4196BioCheckBC-1023Freeb-hCGEIAKitkit4196BioCheckBC-1061b-2MicroglobulinEIAKit§kit4250BioCheckBC-1001TSHEIAKit§kit3800BioCheckBC-1003U-TSH(S-TSH)EIAKit§kit3800BioCheckBC-1005T3EIAKit§kit3656BioCheckBC-1006FreeT3EIAKit§kit3890BioCheckBC-1007T4EIAKit§kit3656BioCheckBC-1008FreeT4EIAKitkit3890BioCheckBC-1081RubellaIgGEIAKit*kit4106BioCheckBC-1083RubellaIgMEIAKit*kit4196BioCheckBC-1085ToxoIgGEIAKit*kit3980BioCheckBC-1087ToxoIgMEIAKit*kit4070BioCheckBC-1089CMVIgGEIAKit*kit3980BioCheckBC-1091CMVIgMEIAKit*kit4070BioCheckBC-1093HSVIIgGEIAKitkit3980BioCheckBC-1095HSVIIgMEIAKitkit4196BioCheckBC-1097HSVIIIgGEIAKitkit3980BioCheckBC-1099HSVIIIgMEIAKitkit4070BioCheckBC-1051H.pyloriIgG(Qualitative)EIAKitkit4016BioCheckBC-1052H.pyloriIgG(Quantitative)EIAKitkit4106BioCheckBC-1053H.pyloriIgM(Qualitative)EIAKitkit4286BioCheckBC-1027hCGEIAKit§kit3800BioCheckBC-1029FSHEIAKit§kit3800BioCheckBC-1031LHEIAKit§kit3800BioCheckBC-1037ProlactinEIAKit§kit3800BioCheckBC-1025HumanFerritinEIAKit§kit3800BioCheckBC-1033HGHEIAKit§kit3800BioCheckBC-1035IgEEIAKit§kit3800BioCheckBC-1203AdiponectinEIAKit*kit7580BioCheckBC-1105TroponinIEIAKit§kit5330BioCheckBC-1117MyoglobinEIAKit§kit4250BioCheckBC-1119HS-CRPEIAKit§kit4250BioCheckBC-1121CK-MBEIAKit*kit6680BioCheckBC-1129MPOEIAKit*kit8480BioCheckBC-1111EstradiolEIAKit§kit4250BioCheckBC-1113ProgesteroneEIAKit§kit4250BioCheckBC-1115TestosteroneEIAKit§kit4250BioCheck我们公司Zda优势是强大的采购，1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，2：货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，3：提供加急服务，一般1-2周到货，超过时限加急费全免4：价格公道，绝大部分价格有优势，当然不能保证1**%产品都有价格优势，5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大交大复旦，中山等100多所大学，ROCHE，阿斯利康，国药，fisher等500多家公司6：我们还是santa,AdvancedBiotechnologiesInc;AthensResearch&Technology,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.FormuMaxScientific,Inc;Genebridege;GlycotopeBiotechnologyGmbH;iduron;InnovativeResearchofAmerica；Ludger；neuroprobe;omicronbio;Polysciences;prospecbi;QA-BIO；quickzyme；RESEARCHDIETS,INC；sterlitech；sysy；TriLinkBioTechnologies,Inc；BIOCHECKINC-biochem;zyagen;......几十家国外公司授权代理7：我们还从事invitrogen，qiagen;abcam;sigma;neb;roche;merck;rnd;BD;GE;pierce;BioLegend....等批发[详细]

  2018-09-29 10:02

  产品样册

  |

  • 
  • 
  • 
  • 

• BioCheck新产品BC-1303 说明书

  世界ding级实验材料供应商BioCheck正式授权上海起发为其ZG代理，BioCheck在一直是行业的标杆,一直为广大科研客户提供Z为优质的产品和服务,上海起发一直秉承为ZG科研客户带来**的产品，**的服务，签约BioCheck就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们Zda的收获BioCheckZG代理,BioCheck上海代理，BioCheck北京代理，BioCheck广东代理，BioCheck江苏代理BioCheck湖北代理,BioCheck天津,BioCheck黑龙江代理,BioCheck内蒙古代理,BioCheck吉林代理,BioCheck福建代理,BioCheck江苏代理,BioCheck浙江代理,BioCheck四川代理BIOCHECK公司由创始人Dr.JohnChen,Medix创立,是一家抓也提供肿瘤标志物，心肌标志物，激素类Z高性价比的抗体的公司。简要原理利用竞争酶联免疫方法，预先在微孔中包被羊抗兔抗体，实验时先后加入氯霉素标准品或待测样本，氯霉素酶标抗原和兔抗氯霉素抗体。经过室温温育，反应液中的兔抗氯霉素抗体与微孔板上的羊抗兔抗体结合，待测样品中的抗原与氯霉素酶标抗原竞争微孔板上的兔抗氯霉素抗体。洗涤后，没有与抗体结合的待测样品中的抗原或酶标抗原被洗去，再加入反应底物，结合的酶标抗原的酶将底物转化为蓝色产物，加入终止液后颜色由蓝色变为黄色。反应完成后，样品中氯霉素含量越多，反应呈色就越浅；反之，样品中氯霉素含量越少，则呈色越深。利用标准曲线可计算出样品中氯霉素含量。技术参数检测蜂蜜、蛋类、牛奶、奶粉、水产品、动物组织(肌肉、肝脏等)、饲料、血清、血浆及尿液样本中存在的氯霉素，定量限可达0.025ppb。No.样品检测下限1蜂蜜0.02ppb(0.02ng/g)2蛋类0.02ppb(0.02ng/g)3牛奶0.002ppb(0.002ng/ml)4奶粉0.012ppb(0.012ng/g)5虾，鱼及肉类0.08ppb(0.08ng/g)6饲料0.08ppb(0.08ng/g)7血清/血浆0.02ppb(0.02ng/ml)8尿液0.04ppb(0.04ng/ml)交叉反应名称百分比氯霉素碱0.4%甲基氯霉素<0.04%回收率No.样品回收率1蜂蜜70%~110%2牛奶90%~130%3蛋，虾，鱼及肉类95%~120%4饲料95%~120%5血清/血浆90%~120%6尿液1**%~130%PD-L1ENZYMEIMMUNOASSAYTESTKITCatalogNumber:BC-1303EnzymeImmunoassayfortheQuantitativeDeterminationofPD-L1ConcentrationinHumanSerumandPlasmaFORRESEARCHUSEONLYNotforuseindiagnosticproceduresINTRODUCTIONProgrammeddeathreceptor-ligand1(PD-L1,B7-H1,CD274)isabiomarkerfromtheB7familythatisexpressedonavarietyofcellsandupregulatedinresponsetopro-inflammatorycytokinessuchasinterferongamma1,2,3.PD-L1interactswithPD-1,aco-receptorexpressedbyexhaustedTcells,toencourageanimmunosuppressivetumormicroenvironmentbydecreasingTcellreceptormediatedproliferationandcytokineproduction4,5.ThePD-1/PD-L1interactionfunctionsasanimmunecheckpointinaprocessknownasimmunoeditingwherethehostimmunesystemeliminateshighlyimmunogenictumorswhileallowinglessimmunogenictumortoevadeit2,6.Multiplesolidtumortypesincludingmelanoma,renalcellcarcinoma,non-smallcelllungcancer,ovarian,andcolorectalcancerutilizethisPD-1/PD-L1immunoeditingmechanism2.CurrenttreatmenthasfocusedonblockingthePD-1/PD-L1interactiontoreducetumorevasionbyinhibitingPD-1,withnewfocusonPD-L1aswell1,2.PRINCIPLEOFTHEASSAYThePD-L1ELISAisbasedontheprincipleofasolidphaseenzyme-linkedimmunosorbentassay.TheassaysystemutilizesauniquemonoclonalantibodypairdirectedagainstadistinctantigenicdeterminantonthePD-L1molecule.Onemousemonoclonalanti-PD-L1antibodyisusedforsolidphaseimmobilization(onthemicrotiterwells).Anothermousemonoclonalanti-PD-L1antibodyconjugatedtohorseradishperoxidase(HRP)isintheenzymeconjugatesolution.Thetestsamplesareallowedtoreactsequentiallywiththetwoantibodies,resultinginthePD-L1moleculestobesandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Aftertwoseparate60-minuteincubationstepsatroomtemperature,thewellsarerinsedwithWashBuffertoremoveunboundlabeledantibodies.TMBReagentisaddedandincubatedfor30minutesunderdarkconditions,resultinginthedevelopmentofabluecolor.ThecolordevelopmentisstoppedwiththeadditionofStopSolution,changingthecolortoyellow.TheconcentrationofPD-L1isdirectlyproportionaltothecolorintensityofthetestsamples.Absorbanceismeasuredspectrophotometricallyat450nm.REAGENTSANDMATERIALSPROVIDEDAntibody-CoatedWells(1plate,96wells)Microtiterwellscoatedwithmousemonoclonalanti-PD-L120ng/mlPD-L1Standard(0.5mL/vial)20ng/mLPD-L1inphosphatebuffer-BSAsolutionwithpreservatives3.StandardandSampleDiluent(30mL/bottle,1bottle)Containsphosphatebuffer-BSAsolutionwithpreservativesEnzymeConjugateReagent(12mL/vial,1vial)Containsmousemonoclonalanti-PD-L1conjugatedtohorseradishperoxidase20XWashBuffer(50mL/bottle,1bottle)PhosphatebufferwithdetergentsTMBReagent(11mL/bottle,1bottle)Containsone-stepTMBsolutionStopSolution(11mL/bottle,1bottle)Containsdilutedhydrochloricacid(1NHCl)STORAGECONDITIONSStoretheunopenedkitat2-8°Cuponreceiptandwhenitisnotinuse,untiltheexpirationshownonthekitlabel.Refertothepackagelabelfortheexpirationdate.Keepmicrotiterplateinasealedbagwithdesiccanttominimizeexposuretodampair.REAGENTPREPARATIONAllreagents首ldbeallowedtoreachroomtemperature(18-25°C)beforeuse.Foreachtestrun,prepareafreshstandardset.Dilute20ng/mLstandardto5ng/mL.Preparetwo-foldserialdilutionsofthe5ng/mLStandardwithStandard/SampleDiluent:5ng/mL:0.15mLof20ng/mL+0.45mLofStandard/SampleDiluent2.5ng/mL:0.25mLof5ng/mL+0.25mLofStandard/SampleDiluent1.25ng/mL:0.25mLof2.5ng/ml+0.25mLofStandard/SampleDiluent0.625ng/mL:0.25mLof1.25ng/mL+0.25mLofStandard/SampleDiluent0.313ng/mL:0.25mLof0.625ng/mL+0.25mLofStandard/SampleDiluent0.156ng/mL:0.25mLof0.313ng/mL+0.25mLofStandardDiluent0.078ng/mL:0.25mLof0.156ng/mL+0.25mLofStandardDiluent0ng/mL:0.25mLofStandardDiluentPatientsamplesneedtobediluted4-foldpriortouse.Prepareaseriesofsmalltubes(i.e.,1.5mLmicrocentrifugetubes)andmix60Lofserumwith180LStandard/SampleDiluent.WorkingWashBuffer:Preparationof1XWashBufferfrom20XStock.Add50mLof20XWashBufferStockto950mLofDIH2O.TheWorkingWashBufferisstableat2-8°Cfor30days.NOTE:Anycrystalsthatmaybepresentduetohighsaltconcentrationmustberedissolvedatroomtemperaturebeforemakingthedilution.ASSAYPROCEDUREPrepareStandards.SeeReagentPreparation.Dilutesamples1:4dilution.SeeReagentPreparation.Securethedesirednumberofcoatedwellsintheholder.Dispense100mLofPD-L1standards,andDILUTEDspecimensintotheappropriatewells.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300mLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLofPD-L1WorkingEnzymeConjugateReagentintoeachwell.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300uLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLTMBsolutionintoeachwell.Incubatefor30minutesatroomtemperature(18-25°C).Stopthereactionbyadding100mLofStopSolutionintoeachwell.Gentlymixfor30seconds.Itisimportanttomakesurethatallthebluecolorchangestoyellowcolorcompletely.Readabsorbanceat450nmwithamicrotiterwellreaderwithin15minutes.CALCULATIONOFRESULTSCalculatethemeanabsorbancevalue(OD450)foreachsetofreferencestandards,controlsandsamples.Constructastandardcurvebyplottingthemeanabsorbanceobtainedforeachreferencestandardagainstitsconcentrationinng/mlongraphpaper,withabsorbanceonthevertical(y)axisandconcentrationonthehorizontal(x)axis.ThecorrespondingconcentrationofPD-L1(ng/mL)canbedeterminedfromthestandardcurveusingthemeanabsorbancevalueforeachsample.Dependingonexperienceand/ortheavailabilityofcomputercapability,othermethodsofdatareductionmaybeemployed.Theobtainedvaluesofthesamples首ldbemultipliedbythedilutionfactorof4toobtainPD-L1resultsinng/ml.EXAMPLEOFSTANDARDCURVEResultsofatypicalstandardrunwithabsorbencyreadingsat450nmshownontheYaxisagainstPD-L1concentrationsshownontheXaxis.NOTE:Thisstandardcurveisforthepurposeofillustrationonly,and首ldnotbeusedtocalculateunknowns.Eachlaboratorymustgenerateitsowndataandstandardcurveineachexperiment.PD-L1Absorbance(ng/ml)(450nm)00.0420.0780.1410.1560.2360.3130.4150.6250.7261.251.2832.52.10053.053nm3.53(4502.52Absorbance1.510.50012345PD-L1Conc.(ng/ml)PERFORMANCECHARACTERISTICSSensitivityTheminimumdetectableconcentrationofthePD-L1ELISAassayasmeasuredby2SDfromthemeanofazerostandardisestimatedtobe0.02ng/ml.PrecisionIntra-AssayPrecisionWithin-runprecisionwasdeterminedbyreplicatedeterminationsofthreedifferentsamplesinoneassay.Within-assayvariabilityisshownbelow:Sample123#Replicates242424MeanPD-L1(ng/mL)1.53.07.4S.D.0.040.050.18C.V.(%)2.4%1.6%2.4%2Inter-AssayPrecisionBetween-runprecisionwasdeterminedbyreplicatemeasurementsofthreedifferentsamplesoveraseriesofindividuallycalibratedassays.Between-assayvariabilityisshownbelow:Sample123#Replicates202020MeanPD-L1(ng/mL)1.63.07.4S.D.0.040.120.26C.V.(%)2.5%4.1%3.5%RecoveryandLinearityStudiesRecoverySampleswerespikedwithknownPD-L1levelsandassayedinduplicate.Themeanrecoverywas90%.SampleEXPECTEDOBSERVED%RECOVERY[PD-L1][PD-L1](ng/mL)(ng/mL)121.890%254.692%3108.989%LinearityThreesampleswereseriallydilutedtodeterminelinearity.Themeanrecoverywas110.3%.#DilutionExpectedObservedConc.(ng/ml)Conc.(ng/ml)%Expected1.1:41.61.6N/A1:81.7106.3%1:161.7106.3%1:321.8112.5%Mean=108.4%2.1:43.73.7N/A1:84.0108.1%1:164.0108.1%1:324.1110.8%Mean=109.0%3.1:47.47.4N/A1:88.3112.2%1:168.6116.2%1:328.3112.2%Mean=113.5%REFERENCESHerbst,RoyS.,etal."Predictivecorrelatesofresponsetotheanti-PD-L1antibodyMPDL3280Aincancerpatients."Nature515.7528(2014):563.Patel,SandipPravin,andRazelleKurzrock."PD-L1expressionasapredictivebiomarkerincancerimmunotherapy."Molecularcancertherapeutics14.4(2015):847-856.Topalian,SuzanneL.,CharlesG.Drake,andDrewM.Pardoll."TargetingthePD-1/B7-H1(PD-L1)pathwaytoactivateanti-tumorimmunity."Currentopinioninimmunology24.2(2012):207-212.Blank,Christian,andAndreasMackensen."ContributionofthePD-L1/PD-1pathwaytoT-cellexhaustion:anupdateonimplicationsforchronicinfectionsandtumorevasion."Cancerimmunology,immunotherapy56.5(2007):739-745.Barber,DanielL.,etal."RestoringfunctioninexhaustedCD8Tcellsduringchronicviralinfection."Nature439.7077(2006):682.Teng,MicheleWL,etal."ClassifyingcancersbasedonT-cellinfiltrationandPD-L1."Cancerresearch75.11(2015):2139-2145.部分Zxin产品如下：货号品名Tests/Kit规格品牌70748MouseMonoclonalanti-humanPD-1(Capture)Purified0.1/0.5/1mgBioCheck70749MouseMonoclonalanti-humanPD-1(Capture)Purified0.1/0.5/1mgBioCheck70750MouseMonoclonalanti-humanPD-1(Detection)Purified0.1/0.5/1mgBioCheck70751MouseMonoclonalanti-humanPD-L1(Capture)Purified0.1/0.5/1mgBioCheck70752MouseMonoclonalanti-humanPD-L1(Capture)Purified0.1/0.5/1mgBioCheck70753MouseMonoclonalanti-humanPD-L1(Detection)Purified0.1/0.5/1mgBioCheck70745Mousemonoclonalanti-humanLp-PLA2(Capture)Purified0.5mgBioCheck70746Mousemonoclonalanti-humanLp-PLA2(Detection)Purified0.5mgBioCheck70747Mousemonoclonalanti-humanLp-PLA2(Detection)Purified0.5mgBioCheck我们公司Zda优势是强大的采购，1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，2：货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，3：提供加急服务，一般1-2周到货，超过时限加急费全免4：价格公道，绝大部分价格有优势，当然不能保证1**%产品都是价格Zdi,因为价格Zdi意味着没有服务.5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大交大复旦，中山等100多所大学，ROCHE，阿斯利康，国药，fisher等500多家公司6：我们du家代理的品牌有：AntibodyResearchCorporation，arcticzymes，Biorelevant，AmberGen,Inc.，clemente-associates，clodronateliposomes，ColumbiaBiosciences，enzymeresearch，GeneBridgesGmbH，Genovis，AmberGen,Inc.BiotechnologyGmbH，HaematologicTechnologiesHTIHaemtech，hookelabs，Immudex，InnovativeResearchofAmerica，inspiralis，ListBiologicalLaboratories,Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-FreezBiologicals，pentapharm，progen，ProteinArk，QA-Bio,Inc，QA-Bio,IncQuickZymeBiosciences，Teknova，TriLinkBioTechnologies,Inc.，ZyagenLaboratories等7：我们还是invitrogen,qiagen,MidlandBioProductsCorporationam,sigma;neb,roche,merck,rnd,BD,GE,pierce,BioLegend等知名批发，欢迎合作。[详细]

  2018-09-29 10:02

  产品样册

  |

  • 
  • 
  • 
  • 

• BioCheck新产品BC-1301 说明书

  世界ding级实验材料供应商BioCheck正式授权上海起发为其ZG代理，BioCheck在一直是行业的标杆,一直为广大科研客户提供Z为优质的产品和服务,上海起发一直秉承为ZG科研客户带来**的产品，**的服务，签约BioCheck就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们Zda的收获BioCheckZG代理,BioCheck上海代理，BioCheck北京代理，BioCheck广东代理，BioCheck江苏代理BioCheck湖北代理,BioCheck天津,BioCheck黑龙江代理,BioCheck内蒙古代理,BioCheck吉林代理,BioCheck福建代理,BioCheck江苏代理,BioCheck浙江代理,BioCheck四川代理BIOCHECK公司由创始人Dr.JohnChen,Medix创立,是一家抓也提供肿瘤标志物，心肌标志物，激素类Z高性价比的抗体的公司。简要原理利用竞争酶联免疫方法，预先在微孔中包被羊抗兔抗体，实验时先后加入氯霉素标准品或待测样本，氯霉素酶标抗原和兔抗氯霉素抗体。经过室温温育，反应液中的兔抗氯霉素抗体与微孔板上的羊抗兔抗体结合，待测样品中的抗原与氯霉素酶标抗原竞争微孔板上的兔抗氯霉素抗体。洗涤后，没有与抗体结合的待测样品中的抗原或酶标抗原被洗去，再加入反应底物，结合的酶标抗原的酶将底物转化为蓝色产物，加入终止液后颜色由蓝色变为黄色。反应完成后，样品中氯霉素含量越多，反应呈色就越浅；反之，样品中氯霉素含量越少，则呈色越深。利用标准曲线可计算出样品中氯霉素含量。技术参数检测蜂蜜、蛋类、牛奶、奶粉、水产品、动物组织(肌肉、肝脏等)、饲料、血清、血浆及尿液样本中存在的氯霉素，定量限可达0.025ppb。No.样品检测下限1蜂蜜0.02ppb(0.02ng/g)2蛋类0.02ppb(0.02ng/g)3牛奶0.002ppb(0.002ng/ml)4奶粉0.012ppb(0.012ng/g)5虾，鱼及肉类0.08ppb(0.08ng/g)6饲料0.08ppb(0.08ng/g)7血清/血浆0.02ppb(0.02ng/ml)8尿液0.04ppb(0.04ng/ml)交叉反应名称百分比氯霉素碱0.4%甲基氯霉素<0.04%回收率No.样品回收率1蜂蜜70%~110%2牛奶90%~130%3蛋，虾，鱼及肉类95%~120%4饲料95%~120%5血清/血浆90%~120%6尿液1**%~130%PD-1ENZYMEIMMUNOASSAYTESTKITCatalogNumber:BC-1301EnzymeImmunoassayfortheQuantitativeDeterminationofPD-1ConcentrationinHumanSerumandPlasmaFORRESEARCHUSEONLYNotforuseindiagnosticproceduresINTRODUCTIONProgrammedcelldeathprotein1,(PD-1,CD279,PDCD1),isacellsurfacereceptorthatiscriticalfortheregulationofTcellinflammatoryactivityandmaintainsperipheraltoleranceintheimmunesystem(1).WithanextracellularIgVdomain,atransmembranedomain,andanintracellulartailwithtwophosphorylationsites(SHP-1andSHP-2),PD-1isabletointeractwithligandsPD-L1andPD-L2todown-regulatetheimmunesystemandsuppressTcellactivity(2).Uponantigenrecognition,anactivatedTcellexpressesPD-1onitssurfaceandproducesinterferons,whichinducesexpressionofPD-L1,therebypromotingapoptosisorcelldeath.(3)Howeverwhenhijackedbytumorcells,aberrantexpressionofPD-1ligandsinhibitsimmune-modulatoryTcellactivation,leadingtodiseaseprogression(4).ElevatedlevelsofPD-1inserumandplasmahavebeenassociatedwithrheumatoidarthritisandskinsclerosis(5,6).Also,PD-1ispredominantlyexpressedbytumorinfiltratingTcells(7).FurtherresearchimpliesthatusingmonoclonalantibodiestotargetthePD-1immunologiccheckpointhascontributedtobreakthroughprogressinunderstandingandtreatingcancerssuchasmelanomaandothervariousnon-smallcelllungcancer(4).PRINCIPLEOFTHEASSAYThePD-1ELISAisbasedontheprincipleofasolidphaseenzyme-linkedimmunosorbentassay.TheassaysystemutilizesauniquemonoclonalantibodypairdirectedagainstadistinctantigenicdeterminantonthePD-1molecule.Onemousemonoclonalanti-PD-1antibodyisusedforsolidphaseimmobilization(onthemicrotiterwells).Anothermousemonoclonalanti-PD-1antibodyconjugatedtohorseradishperoxidase(HRP)isintheenzymeconjugatesolution.Thetestsamplesareallowedtoreactsequentiallywiththetwoantibodies,resultinginthePD-1moleculestobesandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Aftertwoseparate60-minuteincubationstepsatroomtemperature,thewellsarerinsedwithWashBuffertoremoveunboundlabeledantibodies.TMBReagentisaddedandincubatedfor30minutesunderdarkconditions,resultinginthedevelopmentofabluecolor.ThecolordevelopmentisstoppedwiththeadditionofStopSolution,changingthecolortoyellow.TheconcentrationofPD-1isdirectlyproportionaltothecolorintensityofthetestsamples.Absorbanceismeasuredspectrophotometricallyat450nm.REAGENTSANDMATERIALSPROVIDEDAntibody-CoatedWells(1plate,96wells)Microtiterwellscoatedwithmousemonoclonalanti-PD-150ng/mlPD-1Standard(0.5mL/vial)50ng/mLPD-1inphosphatebuffer-BSAsolutionwithpreservatives3.StandardandSampleDiluent(30mL/bottle,1bottle)Containsphosphatebuffer-BSAsolutionwithpreservativesEnzymeConjugateReagent(12mL/vial,1vial)Containsmousemonoclonalanti-PD-1conjugatedtohorseradishperoxidase20XWashBuffer(50mL/bottle,1bottle)PhosphatebufferwithdetergentsTMBReagent(11mL/bottle,1bottle)Containsone-stepTMBsolutionStopSolution(11mL/bottle,1bottle)Containsdilutedhydrochloricacid(1NHCl)STORAGECONDITIONSStoretheunopenedkitat2-8°Cuponreceiptandwhenitisnotinuse,untiltheexpirationshownonthekitlabel.Refertothepackagelabelfortheexpirationdate.Keepmicrotiterplateinasealedbagwithdesiccanttominimizeexposuretodampair.REAGENTPREPARATIONAllreagents首ldbeallowedtoreachroomtemperature(18-25°C)beforeuse.Foreachtestrun,prepareafreshstandardset.Dilute50ng/mLto10ng/mLwithStandard/Samplediluent.Preparetwo-foldserialdilutionsofthe10ng/mLStandardwithStandard/SampleDiluent:10ng/mL:0.12mLof50ng/mL+0.48mLofStandard/SampleDiluent5ng/mL:0.25mLof10ng/mL+0.25mLofStandard/SampleDiluent2.5ng/mL:0.25mLof5ng/mL+0.25mLofStandard/SampleDiluent1.25ng/mL:0.25mLof2.5ng/ml+0.25mLofStandard/SampleDiluent0.625ng/mL:0.25mLof1.25ng/mL+0.25mLofStandard/SampleDiluent0.313ng/mL:0.25mLof0.625ng/mL+0.25mLofStandard/SampleDiluent0.156ng/mL:0.25mLof0.313ng/mL+0.25mLofStandardDiluent0ng/mL:0.25mLofStandardDiluentPatientsamplesneedtobediluted4-foldpriortouse.Prepareaseriesofsmalltubes(i.e.,1.5mLmicrocentrifugetubes)andmix60Lofserumwith180LStandard/SampleDiluent.WorkingWashBuffer:Preparationof1XWashBufferfrom20XStock.Add50mLof20XWashBufferStockto950mLofDIH2O.TheWorkingWashBufferisstableat2-8°Cfor30days.NOTE:Anycrystalsthatmaybepresentduetohighsaltconcentrationmustberedissolvedatroomtemperaturebeforemakingthedilution.ASSAYPROCEDUREPrepareStandards.SeeReagentPreparation.Dilutesamples1:4dilution.SeeReagentPreparation.Securethedesirednumberofcoatedwellsintheholder.Dispense100mLofPD-1standards,andDILUTEDspecimensintotheappropriatewells.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300mLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLofPD-1WorkingEnzymeConjugateReagentintoeachwell.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300uLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLTMBsolutionintoeachwell.Incubatefor30minutesatroomtemperature(18-25°C).Stopthereactionbyadding100mLofStopSolutionintoeachwell.Gentlymixfor30seconds.Itisimportanttomakesurethatallthebluecolorchangestoyellowcolorcompletely.Readabsorbanceat450nmwithamicrotiterwellreaderwithin15minutes.CALCULATIONOFRESULTSCalculatethemeanabsorbancevalue(OD450)foreachsetofreferencestandards,controlsandsamples.Constructastandardcurvebyplottingthemeanabsorbanceobtainedforeachreferencestandardagainstitsconcentrationinng/mlongraphpaper,withabsorbanceonthevertical(y)axisandconcentrationonthehorizontal(x)axis.ThecorrespondingconcentrationofPD-1(ng/mL)canbedeterminedfromthestandardcurveusingthemeanabsorbancevalueforeachsample.Dependingonexperienceand/ortheavailabilityofcomputercapability,othermethodsofdatareductionmaybeemployed.Theobtainedvaluesofthesamples首ldbemultipliedbythedilutionfactorof4toobtainPD-1resultsinng/ml.EXAMPLEOFSTANDARDCURVEResultsofatypicalstandardrunwithabsorbencyreadingsat450nmshownontheYaxisagainstPD-1concentrationsshownontheXaxis.NOTE:Thisstandardcurveisforthepurposeofillustrationonly,and首ldnotbeusedtocalculateunknowns.Eachlaboratorymustgenerateitsowndataandstandardcurveineachexperiment.PD-1Absorbance(ng/ml)(450nm)00.0890.1560.1380.3130.1900.6250.2841.250.4812.50.85151.581102.776nm32.5(4502Absorbance1.510.500246810PD-1Conc.(ng/ml)PERFORMANCECHARACTERISTICSSensitivityTheminimumdetectableconcentrationofthePD-1ELISAassayasmeasuredby2SDfromthemeanofazerostandardisestimatedtobe0.15ng/ml.PrecisionIntra-AssayPrecisionWithin-runprecisionwasdeterminedbyreplicatedeterminationsofthreedifferentsamplesinoneassay.Within-assayvariabilityisshownbelow:Sample123#Replicates242424MeanPD-1(ng/mL)8.413.222.9S.D.0.61.00.9C.V.(%)7.0%7.2%3.8%.2Inter-AssayPrecisionBetween-runprecisionwasdeterminedbyreplicatemeasurementsofthreedifferentsamplesoveraseriesofindividuallycalibratedassays.Between-assayvariabilityisshownbelow:Sample123#Replicates202020MeanPD-1(ng/mL)9.113.525.3S.D.0.81.10.9C.V.(%)9.2%7.93.7RecoveryandLinearityStudiesRecoverySampleswerespikedwithknownPD-1levelsandassayedinduplicate.Themeanrecoverywas105.3%.PAIREXPECTEDOBSERVED%RECOVERYNUMBER[PD-1][PD-1](ng/mL)(ng/mL)188.8110.0%212.513.1104.8%32525.3101.2%LinearityThreesampleswereseriallydilutedtodeterminelinearity.Themeanrecoverywas99.6%.#DilutionExpectedObservedConc.(ng/ml)Conc.(ng/ml)%Expected1.1:210.1114.8%1:48.88.8N/A1:87.888.6%Mean=101.7%2.1:214.5110.6%1:413.113.1N/A1:811.688.5%Mean=99.6%3.1:226.5104.7%1:425.325.3N/A1:823.090.0%Mean=97.4%REFERENCESFife,B.T.,&Pauken,K.E.(2011).TheroleofthePD-1pathwayinautoimmunityandperipheraltolerance.AnnalsoftheNewYorkAcademyofSciences,1217(1),45-59.Riella,L.V.,Paterson,A.M.,Sharpe,A.H.,&Chandraker,A.(2012).RoleofthePD-1PathwayintheImmuneResponse.AmericanJournalofTransplantation:OfficialJournaloftheAmericanSocietyofTransplantationandtheAmericanSocietyofTransplantSurgeons,12(10),25752587.Zak,Krzysztof&Kitel,Radosaw&Przetocka,Sara&Golik,Przemysaw&Guzik,Katarzyna&Musielak,Bogdan&Dm领,Alexander&Dubin,Grzegorz&Holak,Tad.(2015).StructureoftheComplexofHumanProgrammedDeath1,PD-1,andItsLigandPD-L1.Structure.23..10.1016/j.str.2015.09.010.ZoranGatalica,CarrieSnyder,ToddManey,AnatoleGhazalpour,DanielHolterman,NianqingXiao,PeggyOverberg,IngaRose,GargiD.Basu,SemirVranic,HenryT.Lynch,DanielD.VonHoffandOmidHamid.ProgrammedCellDeath1(PD-1)andItsLigand(PD-L1)inCommonCancersandTheirCorrelationwithMolecularCancerType.CancerEpidemiolBiomarkersPrev.December12014(23)(12)2965-2970.Greisen,S.,Rasmussen,T.,Stengaard-Pedersen,K.,Hetland,M.,Hrslev-Petersen,K.,Hvid,M.,&Deleuran,B.(2013).Increasedsolubleprogrammeddeath-1(sPD-1)isassociatedwithdiseaseactivityandradiographicprogressioninearlyrheumatoidarthritis.ScandinavianJournalofRheumatology,43(2),101-108.Yanaba,K.,Hayashi,M.,Yoshihara,Y.,&Nakagawa,H.(2016).Serumlevelsofsolubleprogrammeddeath-1andprogrammeddeathligand-1insystemicsclerosis:Associationwithextentofskinsclerosis.TheJournalofDermatology,43(8),954-957.Ahmadzadeh,M.,Johnson,L.A.,Heemskerk,B.,Wunderlich,J.R.,Dudley,M.E.,White,D.E.,&Rosenberg,S.A.(2009).TumorantigenspecificCD8TcellsinfiltratingthetumorexpresshighlevelsofPD-1andarefunctionallyimpaired.Blood,114(8),15371544.部分产品Zxin报价如下：货号品名规格价格品牌BC-1009AFPEIAKitkit3980BioCheckBC-1011CEAEIAKitkit4016BioCheckBC-1013CA125EIAKitkit4970BioCheckBC-1015CA15-3EIAKitkit4970BioCheckBC-1017CA19-9EIAKitkit4970BioCheckBC-1019PSAEIAKit*kit3962BioCheckBC-1021FreePSAEIAKit*kit4196BioCheckBC-1023Freeb-hCGEIAKitkit4196BioCheckBC-1061b-2MicroglobulinEIAKit§kit4250BioCheckBC-1001TSHEIAKit§kit3800BioCheckBC-1003U-TSH(S-TSH)EIAKit§kit3800BioCheckBC-1005T3EIAKit§kit3656BioCheckBC-1006FreeT3EIAKit§kit3890BioCheckBC-1007T4EIAKit§kit3656BioCheckBC-1008FreeT4EIAKitkit3890BioCheckBC-1081RubellaIgGEIAKit*kit4106BioCheckBC-1083RubellaIgMEIAKit*kit4196BioCheckBC-1085ToxoIgGEIAKit*kit3980BioCheckBC-1087ToxoIgMEIAKit*kit4070BioCheckBC-1089CMVIgGEIAKit*kit3980BioCheckBC-1091CMVIgMEIAKit*kit4070BioCheckBC-1093HSVIIgGEIAKitkit3980BioCheckBC-1095HSVIIgMEIAKitkit4196BioCheckBC-1097HSVIIIgGEIAKitkit3980BioCheckBC-1099HSVIIIgMEIAKitkit4070BioCheckBC-1051H.pyloriIgG(Qualitative)EIAKitkit4016BioCheckBC-1052H.pyloriIgG(Quantitative)EIAKitkit4106BioCheckBC-1053H.pyloriIgM(Qualitative)EIAKitkit4286BioCheckBC-1027hCGEIAKit§kit3800BioCheckBC-1029FSHEIAKit§kit3800BioCheckBC-1031LHEIAKit§kit3800BioCheckBC-1037ProlactinEIAKit§kit3800BioCheckBC-1025HumanFerritinEIAKit§kit3800BioCheckBC-1033HGHEIAKit§kit3800BioCheckBC-1035IgEEIAKit§kit3800BioCheckBC-1203AdiponectinEIAKit*kit7580BioCheckBC-1105TroponinIEIAKit§kit5330BioCheckBC-1117MyoglobinEIAKit§kit4250BioCheckBC-1119HS-CRPEIAKit§kit4250BioCheckBC-1121CK-MBEIAKit*kit6680BioCheckBC-1129MPOEIAKit*kit8480BioCheckBC-1111EstradiolEIAKit§kit4250BioCheckBC-1113ProgesteroneEIAKit§kit4250BioCheckBC-1115TestosteroneEIAKit§kit4250BioCheck我们公司Zda优势是强大的采购，1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，2：货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，3：提供加急服务，一般1-2周到货，超过时限加急费全免4：价格公道，绝大部分价格有优势，当然不能保证1**%产品都有价格优势，5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大交大复旦，中山等100多所大学，ROCHE，阿斯利康，国药，fisher等500多家公司6：我们还是santa,AdvancedBiotechnologiesInc;AthensResearch&Technology,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.FormuMaxScientific,Inc;Genebridege;GlycotopeBiotechnologyGmbH;iduron;InnovativeResearchofAmerica；Ludger；neuroprobe;omicronbio;Polysciences;prospecbi;QA-BIO；quickzyme；RESEARCHDIETS,INC；sterlitech；sysy；TriLinkBioTechnologies,Inc；BIOCHECKINC-biochem;zyagen;......几十家国外公司授权代理7：我们还从事invitrogen，qiagen;abcam;sigma;neb;roche;merck;rnd;BD;GE;pierce;BioLegend....等批发[详细]

  2018-09-29 10:02

  产品样册

  |

  • 
  • 
  • 
  • 

• h-h-c 新产品 NATRSV-6C 说明书

  世界ding级实验材料供应商HelveticaHealthCare正式授权上海起发为其ZG代理，HelveticaHealthCare在一直是行业的标杆,一直为广大科研客户提供Z为优质的产品和服务,上海起发一直秉承为ZG科研客户带来**的产品，**的服务，签约HelveticaHealthCare就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们Zda的收获HelveticaHealthCareZG代理,HelveticaHealthCare上海代理，HelveticaHealthCare北京代理，HelveticaHealthCare广东代理，HelveticaHealthCare江苏代理HelveticaHealthCare湖北代理,HelveticaHealthCare天津,HelveticaHealthCare黑龙江代理,HelveticaHealthCare内蒙古代理,HelveticaHealthCare吉林代理,HelveticaHealthCare福建代理,HelveticaHealthCare江苏代理,HelveticaHealthCare浙江代理,HelveticaHealthCare四川代理,HelveticaHealthCare是一家广泛的YL保健公司，致力于与客户合作，提供创新的产品和技术来管理健康和提高生活质量。我们专注于研究产品和诊断，ZD关注用于核酸测试的分子控制和校准器。我们为许多商业分子系统提供控制。我们还可以获得大量的临床标本，并可以提供来自HIV，HCV和HBV无症状供体的多种血清转化材料。我们公司位于瑞士，在日内瓦，在欧洲心脏地区的国际城市，以生活质量和高水平的专业精神而着称。我们位于瑞士生物瓦伦西亚，为科学研究和开发提供了高度的定位环境。世界上许多领xian的制药公司和非政府组织（包括世界卫生组织）在内的很近。我们经验丰富的团队在我们的实践和透明的业务方法中反映了这些价值我们致力于追求zhuo越，产品质量和可靠性，合作伙伴的选择以及我们为客户提供的个性化服务。NATtrolRSVPositiveControlNATtrolRSV阳性对照产品编号：NATRSV-6C产品描述平台：造父亲GeneXpert;测试：RSV-FLUA/B/+2°至+8°C附加信息重量0.003公斤单位体积6x0.5ml相关产品NATtrol艰难梭菌阴性对照硝基葡萄球菌MSSA;-mecA，外部运行控制，低NATtrol流感A/B阳性对照NATtrolEBV控制目标：10,000cp-ml[详细]

  2018-10-06 10:00

  产品样册

  |

  • 
  • 
  • 
  • 

• h-h-c 新产品 NATFLUAB-6C 说明书

  世界ding级实验材料供应商HelveticaHealthCare正式授权上海起发为其ZG代理，HelveticaHealthCare在一直是行业的标杆,一直为广大科研客户提供Z为优质的产品和服务,上海起发一直秉承为ZG科研客户带来**的产品，**的服务，签约HelveticaHealthCare就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们Zda的收获HelveticaHealthCareZG代理,HelveticaHealthCare上海代理，HelveticaHealthCare北京代理，HelveticaHealthCare广东代理，HelveticaHealthCare江苏代理HelveticaHealthCare湖北代理,HelveticaHealthCare天津,HelveticaHealthCare黑龙江代理,HelveticaHealthCare内蒙古代理,HelveticaHealthCare吉林代理,HelveticaHealthCare福建代理,HelveticaHealthCare江苏代理,HelveticaHealthCare浙江代理,HelveticaHealthCare四川代理,HelveticaHealthCare是一家广泛的YL保健公司，致力于与客户合作，提供创新的产品和技术来管理健康和提高生活质量。我们专注于研究产品和诊断，ZD关注用于核酸测试的分子控制和校准器。我们为许多商业分子系统提供控制。我们还可以获得大量的临床标本，并可以提供来自HIV，HCV和HBV无症状供体的多种血清转化材料。我们公司位于瑞士，在日内瓦，在欧洲心脏地区的国际城市，以生活质量和高水平的专业精神而着称。我们位于瑞士生物瓦伦西亚，为科学研究和开发提供了高度的定位环境。世界上许多领xian的制药公司和非政府组织（包括世界卫生组织）在内的很近。我们经验丰富的团队在我们的实践和透明的业务方法中反映了这些价值我们致力于追求zhuo越，产品质量和可靠性，合作伙伴的选择以及我们为客户提供的个性化服务。NATtrolInfluenzaA/BPositiveControlNATtrol流感A/B阳性对照产品编号：NATFLUAB-6C产品描述平台：GeneXpert;测试：FLUA/B-RSV/+2°至+8°C。附加信息重量0.003公斤单位体积6x0.5ml相关产品MixedTiterBacteriaPanelforPlateletsNATtrolN.gonorrhoeaePositiveControlNATtrolEBVControlTarget:10,000cpmlNATtrolStaphylococcusaureus(MRSA)ExternalRunControl-Medium[详细]

  2018-10-06 10:00

  产品样册

  |

  • 
  • 
  • 
  • 

• h-h-c 新产品 NATCXVA9-6C 说明书

  世界ding级实验材料供应商HelveticaHealthCare正式授权上海起发为其ZG代理，HelveticaHealthCare在一直是行业的标杆,一直为广大科研客户提供Z为优质的产品和服务,上海起发一直秉承为ZG科研客户带来**的产品，**的服务，签约HelveticaHealthCare就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们Zda的收获HelveticaHealthCareZG代理,HelveticaHealthCare上海代理，HelveticaHealthCare北京代理，HelveticaHealthCare广东代理，HelveticaHealthCare江苏代理HelveticaHealthCare湖北代理,HelveticaHealthCare天津,HelveticaHealthCare黑龙江代理,HelveticaHealthCare内蒙古代理,HelveticaHealthCare吉林代理,HelveticaHealthCare福建代理,HelveticaHealthCare江苏代理,HelveticaHealthCare浙江代理,HelveticaHealthCare四川代理,HelveticaHealthCare是一家广泛的YL保健公司，致力于与客户合作，提供创新的产品和技术来管理健康和提高生活质量。我们专注于研究产品和诊断，ZD关注用于核酸测试的分子控制和校准器。我们为许多商业分子系统提供控制。我们还可以获得大量的临床标本，并可以提供来自HIV，HCV和HBV无症状供体的多种血清转化材料。我们公司位于瑞士，在日内瓦，在欧洲心脏地区的国际城市，以生活质量和高水平的专业精神而着称。我们位于瑞士生物瓦伦西亚，为科学研究和开发提供了高度的定位环境。世界上许多领xian的制药公司和非政府组织（包括世界卫生组织）在内的很近。我们经验丰富的团队在我们的实践和透明的业务方法中反映了这些价值我们致力于追求zhuo越，产品质量和可靠性，合作伙伴的选择以及我们为客户提供的个性化服务。NATtrolInfluenza/RSVNegativeControlNATtrol流感/RSV阴性对照产品编号：NATCXVA9-6C产品描述平台：造父亲GeneXpert;测试：FLUA/B-RSV/+2°至+8°C。附加信息重量0.003公斤单位体积6x0.5ml相关产品NATtrolMRSAPositiveControlNATtrolClostridiumdifficileNegativeControlNATtrolSalmonellatyphimurium(StoolMatrix)NATtrolSAPositiveControl我们公司Zda优势是强大的采购，1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，2：货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，3：提供加急服务，一般1-2周到货，超过时限加急费全免4：价格公道，绝大部分价格有优势，当然不能保证1**%产品都是价格Zdi,因为价格Zdi意味着没有服务.5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大交大复旦，中山等100多所大学，ROCHE，阿斯利康，国药，fisher等500多家公司6：我们du家代理的品牌有：AntibodyResearchCorporation，arcticzymes，Biorelevant，AmberGen,Inc.，clemente-associates，clodronateliposomes，ColumbiaBiosciences，enzymeresearch，GeneBridgesGmbH，Genovis，AmberGen,Inc.BiotechnologyGmbH，HaematologicTechnologiesHTIHaemtech，hookelabs，Immudex，InnovativeResearchofAmerica，inspiralis，ListBiologicalLaboratories,Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-FreezBiologicals，pentapharm，progen，ProteinArk，QA-Bio,Inc，QA-Bio,IncQuickZymeBiosciences，Teknova，TriLinkBioTechnologies,Inc.，ZyagenLaboratories等7：我们还是invitrogen,qiagen,MidlandBioProductsCorporationam,sigma;neb,roche,merck,rnd,BD,GE,pierce,BioLegend等知名批发，欢迎合作。[详细]

  2018-10-06 10:00

  产品样册

  |

  • 
  • 
  • 
  • 

• 96通道自动化核酸提取仪说明书

  AP-96N 是一套整合的核酸自动制备系统，可以从全血、病毒、组织、植物、细菌和培养细胞等多种生物样本中纯化核酸。凭借智能化预装提取程序、配套的基于生物磁珠的核酸提取试剂盒和耗材，该系统可为实验室提供高效、自动化、高品质核酸纯化解决方案，服务于下游基因分析和分子诊断。[详细]

  2024-09-14 08:18

  操作手册

  |

  • 
  • 
  • 
  • 

• BioCheck新产品货号70747说明书

  世界ding级实验材料供应商BioCheck正式授权上海起发为其ZG代理，BioCheck在一直是行业的标杆,一直为广大科研客户提供Z为优质的产品和服务,上海起发一直秉承为ZG科研客户带来**的产品，**的服务，签约BioCheck就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们Zda的收获BioCheckZG代理,BioCheck上海代理，BioCheck北京代理，BioCheck广东代理，BioCheck江苏代理BioCheck湖北代理,BioCheck天津,BioCheck黑龙江代理,BioCheck内蒙古代理,BioCheck吉林代理,BioCheck福建代理,BioCheck江苏代理,BioCheck浙江代理,BioCheck四川代理BIOCHECK公司由创始人Dr.JohnChen,Medix创立,是一家抓也提供肿瘤标志物，心肌标志物，激素类Z高性价比的抗体的公司。简要原理利用竞争酶联免疫方法，预先在微孔中包被羊抗兔抗体，实验时先后加入氯霉素标准品或待测样本，氯霉素酶标抗原和兔抗氯霉素抗体。经过室温温育，反应液中的兔抗氯霉素抗体与微孔板上的羊抗兔抗体结合，待测样品中的抗原与氯霉素酶标抗原竞争微孔板上的兔抗氯霉素抗体。洗涤后，没有与抗体结合的待测样品中的抗原或酶标抗原被洗去，再加入反应底物，结合的酶标抗原的酶将底物转化为蓝色产物，加入终止液后颜色由蓝色变为黄色。反应完成后，样品中氯霉素含量越多，反应呈色就越浅；反之，样品中氯霉素含量越少，则呈色越深。利用标准曲线可计算出样品中氯霉素含量。技术参数检测蜂蜜、蛋类、牛奶、奶粉、水产品、动物组织(肌肉、肝脏等)、饲料、血清、血浆及尿液样本中存在的氯霉素，定量限可达0.025ppb。No.样品检测下限1蜂蜜0.02ppb(0.02ng/g)2蛋类0.02ppb(0.02ng/g)3牛奶0.002ppb(0.002ng/ml)4奶粉0.012ppb(0.012ng/g)5虾，鱼及肉类0.08ppb(0.08ng/g)6饲料0.08ppb(0.08ng/g)7血清/血浆0.02ppb(0.02ng/ml)8尿液0.04ppb(0.04ng/ml)交叉反应名称百分比氯霉素碱0.4%甲基氯霉素<0.04%回收率No.样品回收率1蜂蜜70%~110%2牛奶90%~130%3蛋，虾，鱼及肉类95%~120%4饲料95%~120%5血清/血浆90%~120%6尿液1**%~130%ProductName:MouseMonoclonalAnti-HumanLipoproteinAssociatedPhospholipaseA2(Lp-PLA2)CatalogNumber:70747Subclass:IgG1ProductForm:PBS,pH7.4with0.09%sodiumazideStorage:At2-8C.DoNotFreeze.Precipitationmayoccuruponfreezing.SuggestedUse:Forenzymeconjugation(detection)inthehumanLpPLA2immunoassayPairingInformation:ThisantibodypairswithMouseMonoclonalAnti-HumanLp-PLA2PartNo.70745部分产品Zxin报价如下：货号品名规格价格品牌BC-1009AFPEIAKitkit3980BioCheckBC-1011CEAEIAKitkit4016BioCheckBC-1013CA125EIAKitkit4970BioCheckBC-1015CA15-3EIAKitkit4970BioCheckBC-1017CA19-9EIAKitkit4970BioCheckBC-1019PSAEIAKit*kit3962BioCheckBC-1021FreePSAEIAKit*kit4196BioCheckBC-1023Freeb-hCGEIAKitkit4196BioCheckBC-1061b-2MicroglobulinEIAKit§kit4250BioCheckBC-1001TSHEIAKit§kit3800BioCheckBC-1003U-TSH(S-TSH)EIAKit§kit3800BioCheckBC-1005T3EIAKit§kit3656BioCheckBC-1006FreeT3EIAKit§kit3890BioCheckBC-1007T4EIAKit§kit3656BioCheckBC-1008FreeT4EIAKitkit3890BioCheckBC-1081RubellaIgGEIAKit*kit4106BioCheckBC-1083RubellaIgMEIAKit*kit4196BioCheckBC-1085ToxoIgGEIAKit*kit3980BioCheckBC-1087ToxoIgMEIAKit*kit4070BioCheckBC-1089CMVIgGEIAKit*kit3980BioCheckBC-1091CMVIgMEIAKit*kit4070BioCheckBC-1093HSVIIgGEIAKitkit3980BioCheckBC-1095HSVIIgMEIAKitkit4196BioCheckBC-1097HSVIIIgGEIAKitkit3980BioCheckBC-1099HSVIIIgMEIAKitkit4070BioCheckBC-1051H.pyloriIgG(Qualitative)EIAKitkit4016BioCheckBC-1052H.pyloriIgG(Quantitative)EIAKitkit4106BioCheckBC-1053H.pyloriIgM(Qualitative)EIAKitkit4286BioCheckBC-1027hCGEIAKit§kit3800BioCheckBC-1029FSHEIAKit§kit3800BioCheckBC-1031LHEIAKit§kit3800BioCheckBC-1037ProlactinEIAKit§kit3800BioCheckBC-1025HumanFerritinEIAKit§kit3800BioCheckBC-1033HGHEIAKit§kit3800BioCheckBC-1035IgEEIAKit§kit3800BioCheckBC-1203AdiponectinEIAKit*kit7580BioCheckBC-1105TroponinIEIAKit§kit5330BioCheckBC-1117MyoglobinEIAKit§kit4250BioCheckBC-1119HS-CRPEIAKit§kit4250BioCheckBC-1121CK-MBEIAKit*kit6680BioCheckBC-1129MPOEIAKit*kit8480BioCheckBC-1111EstradiolEIAKit§kit4250BioCheckBC-1113ProgesteroneEIAKit§kit4250BioCheckBC-1115TestosteroneEIAKit§kit4250BioCheck我们公司Zda优势是强大的采购，1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，2：货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，3：提供加急服务，一般1-2周到货，超过时限加急费全免4：价格公道，绝大部分价格有优势，当然不能保证1**%产品都有价格优势，5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大交大复旦，中山等100多所大学，ROCHE，阿斯利康，国药，fisher等500多家公司6：我们还是santa,AdvancedBiotechnologiesInc;AthensResearch&Technology,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.FormuMaxScientific,Inc;Genebridege;GlycotopeBiotechnologyGmbH;iduron;InnovativeResearchofAmerica；Ludger；neuroprobe;omicronbio;Polysciences;prospecbi;QA-BIO；quickzyme；RESEARCHDIETS,INC；sterlitech；sysy；TriLinkBioTechnologies,Inc；BIOCHECKINC-biochem;zyagen;......几十家国外公司授权代理7：我们还从事invitrogen，qiagen;abcam;sigma;neb;roche;merck;rnd;BD;GE;pierce;BioLegend....等批发[详细]

  2018-09-29 10:02

  产品样册

  |

  • 
  • 
  • 
  • 

• BioCheck新产品货号70746说明书

  世界ding级实验材料供应商BioCheck正式授权上海起发为其ZG代理，BioCheck在一直是行业的标杆,一直为广大科研客户提供Z为优质的产品和服务,上海起发一直秉承为ZG科研客户带来**的产品，**的服务，签约BioCheck就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们Zda的收获BioCheckZG代理,BioCheck上海代理，BioCheck北京代理，BioCheck广东代理，BioCheck江苏代理BioCheck湖北代理,BioCheck天津,BioCheck黑龙江代理,BioCheck内蒙古代理,BioCheck吉林代理,BioCheck福建代理,BioCheck江苏代理,BioCheck浙江代理,BioCheck四川代理BIOCHECK公司由创始人Dr.JohnChen,Medix创立,是一家抓也提供肿瘤标志物，心肌标志物，激素类Z高性价比的抗体的公司。简要原理利用竞争酶联免疫方法，预先在微孔中包被羊抗兔抗体，实验时先后加入氯霉素标准品或待测样本，氯霉素酶标抗原和兔抗氯霉素抗体。经过室温温育，反应液中的兔抗氯霉素抗体与微孔板上的羊抗兔抗体结合，待测样品中的抗原与氯霉素酶标抗原竞争微孔板上的兔抗氯霉素抗体。洗涤后，没有与抗体结合的待测样品中的抗原或酶标抗原被洗去，再加入反应底物，结合的酶标抗原的酶将底物转化为蓝色产物，加入终止液后颜色由蓝色变为黄色。反应完成后，样品中氯霉素含量越多，反应呈色就越浅；反之，样品中氯霉素含量越少，则呈色越深。利用标准曲线可计算出样品中氯霉素含量。技术参数检测蜂蜜、蛋类、牛奶、奶粉、水产品、动物组织(肌肉、肝脏等)、饲料、血清、血浆及尿液样本中存在的氯霉素，定量限可达0.025ppb。No.样品检测下限1蜂蜜0.02ppb(0.02ng/g)2蛋类0.02ppb(0.02ng/g)3牛奶0.002ppb(0.002ng/ml)4奶粉0.012ppb(0.012ng/g)5虾，鱼及肉类0.08ppb(0.08ng/g)6饲料0.08ppb(0.08ng/g)7血清/血浆0.02ppb(0.02ng/ml)8尿液0.04ppb(0.04ng/ml)交叉反应名称百分比氯霉素碱0.4%甲基氯霉素<0.04%回收率No.样品回收率1蜂蜜70%~110%2牛奶90%~130%3蛋，虾，鱼及肉类95%~120%4饲料95%~120%5血清/血浆90%~120%6尿液1**%~130%ProductName:MouseMonoclonalAnti-HumanLipoproteinAssociatedPhospholipaseA2(Lp-PLA2)CatalogNumber:70746Subclass:IgG1ProductForm:PBS,pH7.4with0.09%sodiumazideStorage:At2-8C.DoNotFreeze.Precipitationmayoccuruponfreezing.SuggestedUse:Forenzymeconjugation(detection)inthehumanLpPLA2immunoassay.PairingInformation:ThisantibodypairswithMouseMonoclonalAnti-HumanLp-PLA2PartNo.70745部分产品Zxin报价如下：货号品名规格价格品牌BC-1009AFPEIAKitkit3980BioCheckBC-1011CEAEIAKitkit4016BioCheckBC-1013CA125EIAKitkit4970BioCheckBC-1015CA15-3EIAKitkit4970BioCheckBC-1017CA19-9EIAKitkit4970BioCheckBC-1019PSAEIAKit*kit3962BioCheckBC-1021FreePSAEIAKit*kit4196BioCheckBC-1023Freeb-hCGEIAKitkit4196BioCheckBC-1061b-2MicroglobulinEIAKit§kit4250BioCheckBC-1001TSHEIAKit§kit3800BioCheckBC-1003U-TSH(S-TSH)EIAKit§kit3800BioCheckBC-1005T3EIAKit§kit3656BioCheckBC-1006FreeT3EIAKit§kit3890BioCheckBC-1007T4EIAKit§kit3656BioCheckBC-1008FreeT4EIAKitkit3890BioCheckBC-1081RubellaIgGEIAKit*kit4106BioCheckBC-1083RubellaIgMEIAKit*kit4196BioCheckBC-1085ToxoIgGEIAKit*kit3980BioCheckBC-1087ToxoIgMEIAKit*kit4070BioCheckBC-1089CMVIgGEIAKit*kit3980BioCheckBC-1091CMVIgMEIAKit*kit4070BioCheckBC-1093HSVIIgGEIAKitkit3980BioCheckBC-1095HSVIIgMEIAKitkit4196BioCheckBC-1097HSVIIIgGEIAKitkit3980BioCheckBC-1099HSVIIIgMEIAKitkit4070BioCheckBC-1051H.pyloriIgG(Qualitative)EIAKitkit4016BioCheckBC-1052H.pyloriIgG(Quantitative)EIAKitkit4106BioCheckBC-1053H.pyloriIgM(Qualitative)EIAKitkit4286BioCheckBC-1027hCGEIAKit§kit3800BioCheckBC-1029FSHEIAKit§kit3800BioCheckBC-1031LHEIAKit§kit3800BioCheckBC-1037ProlactinEIAKit§kit3800BioCheckBC-1025HumanFerritinEIAKit§kit3800BioCheckBC-1033HGHEIAKit§kit3800BioCheckBC-1035IgEEIAKit§kit3800BioCheckBC-1203AdiponectinEIAKit*kit7580BioCheckBC-1105TroponinIEIAKit§kit5330BioCheckBC-1117MyoglobinEIAKit§kit4250BioCheckBC-1119HS-CRPEIAKit§kit4250BioCheckBC-1121CK-MBEIAKit*kit6680BioCheckBC-1129MPOEIAKit*kit8480BioCheckBC-1111EstradiolEIAKit§kit4250BioCheckBC-1113ProgesteroneEIAKit§kit4250BioCheckBC-1115TestosteroneEIAKit§kit4250BioCheck我们公司Zda优势是强大的采购，1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，2：货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，3：提供加急服务，一般1-2周到货，超过时限加急费全免4：价格公道，绝大部分价格有优势，当然不能保证1**%产品都有价格优势，5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大交大复旦，中山等100多所大学，ROCHE，阿斯利康，国药，fisher等500多家公司6：我们还是santa,AdvancedBiotechnologiesInc;AthensResearch&Technology,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.FormuMaxScientific,Inc;Genebridege;GlycotopeBiotechnologyGmbH;iduron;InnovativeResearchofAmerica；Ludger；neuroprobe;omicronbio;Polysciences;prospecbi;QA-BIO；quickzyme；RESEARCHDIETS,INC；sterlitech；sysy；TriLinkBioTechnologies,Inc；BIOCHECKINC-biochem;zyagen;......几十家国外公司授权代理7：我们还从事invitrogen，qiagen;abcam;sigma;neb;roche;merck;rnd;BD;GE;pierce;BioLegend....等批发[详细]

  2018-09-29 10:02

  产品样册

  |

  • 
  • 
  • 
  • 

• BioCheck新产品货号70745说明书

  世界ding级实验材料供应商BioCheck正式授权上海起发为其ZG代理，BioCheck在一直是行业的标杆,一直为广大科研客户提供Z为优质的产品和服务,上海起发一直秉承为ZG科研客户带来**的产品，**的服务，签约BioCheck就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们Zda的收获BioCheckZG代理,BioCheck上海代理，BioCheck北京代理，BioCheck广东代理，BioCheck江苏代理BioCheck湖北代理,BioCheck天津,BioCheck黑龙江代理,BioCheck内蒙古代理,BioCheck吉林代理,BioCheck福建代理,BioCheck江苏代理,BioCheck浙江代理,BioCheck四川代理BIOCHECK公司由创始人Dr.JohnChen,Medix创立,是一家抓也提供肿瘤标志物，心肌标志物，激素类Z高性价比的抗体的公司。简要原理利用竞争酶联免疫方法，预先在微孔中包被羊抗兔抗体，实验时先后加入氯霉素标准品或待测样本，氯霉素酶标抗原和兔抗氯霉素抗体。经过室温温育，反应液中的兔抗氯霉素抗体与微孔板上的羊抗兔抗体结合，待测样品中的抗原与氯霉素酶标抗原竞争微孔板上的兔抗氯霉素抗体。洗涤后，没有与抗体结合的待测样品中的抗原或酶标抗原被洗去，再加入反应底物，结合的酶标抗原的酶将底物转化为蓝色产物，加入终止液后颜色由蓝色变为黄色。反应完成后，样品中氯霉素含量越多，反应呈色就越浅；反之，样品中氯霉素含量越少，则呈色越深。利用标准曲线可计算出样品中氯霉素含量。技术参数检测蜂蜜、蛋类、牛奶、奶粉、水产品、动物组织(肌肉、肝脏等)、饲料、血清、血浆及尿液样本中存在的氯霉素，定量限可达0.025ppb。No.样品检测下限1蜂蜜0.02ppb(0.02ng/g)2蛋类0.02ppb(0.02ng/g)3牛奶0.002ppb(0.002ng/ml)4奶粉0.012ppb(0.012ng/g)5虾，鱼及肉类0.08ppb(0.08ng/g)6饲料0.08ppb(0.08ng/g)7血清/血浆0.02ppb(0.02ng/ml)8尿液0.04ppb(0.04ng/ml)交叉反应名称百分比氯霉素碱0.4%甲基氯霉素<0.04%回收率No.样品回收率1蜂蜜70%~110%2牛奶90%~130%3蛋，虾，鱼及肉类95%~120%4饲料95%~120%5血清/血浆90%~120%6尿液1**%~130%ProductName:MouseMonoclonalAnti-HumanLipoproteinAssociatedPhospholipaseA2(Lp-PLA2)CatalogNumber:70745Subclass:IgG1ProductForm:PBS,pH7.4with0.09%sodiumazideStorage:At2-8C.DoNotFreeze.Precipitationmayoccuruponfreezing.SuggestedUse:Forsolidphasecoating(capture)inthehumanLp-PLA2immunoassay.PairingInformation:ThisantibodypairswithMouseMonoclonalAnti-HumanLp-PLA2PartNo.70746orMouseMonoclonalAnti-HumanLp-PLA2PartNo.70747部分产品Zxin报价如下：货号品名规格价格品牌BC-1009AFPEIAKitkit3980BioCheckBC-1011CEAEIAKitkit4016BioCheckBC-1013CA125EIAKitkit4970BioCheckBC-1015CA15-3EIAKitkit4970BioCheckBC-1017CA19-9EIAKitkit4970BioCheckBC-1019PSAEIAKit*kit3962BioCheckBC-1021FreePSAEIAKit*kit4196BioCheckBC-1023Freeb-hCGEIAKitkit4196BioCheckBC-1061b-2MicroglobulinEIAKit§kit4250BioCheckBC-1001TSHEIAKit§kit3800BioCheckBC-1003U-TSH(S-TSH)EIAKit§kit3800BioCheckBC-1005T3EIAKit§kit3656BioCheckBC-1006FreeT3EIAKit§kit3890BioCheckBC-1007T4EIAKit§kit3656BioCheckBC-1008FreeT4EIAKitkit3890BioCheckBC-1081RubellaIgGEIAKit*kit4106BioCheckBC-1083RubellaIgMEIAKit*kit4196BioCheckBC-1085ToxoIgGEIAKit*kit3980BioCheckBC-1087ToxoIgMEIAKit*kit4070BioCheckBC-1089CMVIgGEIAKit*kit3980BioCheckBC-1091CMVIgMEIAKit*kit4070BioCheckBC-1093HSVIIgGEIAKitkit3980BioCheckBC-1095HSVIIgMEIAKitkit4196BioCheckBC-1097HSVIIIgGEIAKitkit3980BioCheckBC-1099HSVIIIgMEIAKitkit4070BioCheckBC-1051H.pyloriIgG(Qualitative)EIAKitkit4016BioCheckBC-1052H.pyloriIgG(Quantitative)EIAKitkit4106BioCheckBC-1053H.pyloriIgM(Qualitative)EIAKitkit4286BioCheckBC-1027hCGEIAKit§kit3800BioCheckBC-1029FSHEIAKit§kit3800BioCheckBC-1031LHEIAKit§kit3800BioCheckBC-1037ProlactinEIAKit§kit3800BioCheckBC-1025HumanFerritinEIAKit§kit3800BioCheckBC-1033HGHEIAKit§kit3800BioCheckBC-1035IgEEIAKit§kit3800BioCheckBC-1203AdiponectinEIAKit*kit7580BioCheckBC-1105TroponinIEIAKit§kit5330BioCheckBC-1117MyoglobinEIAKit§kit4250BioCheckBC-1119HS-CRPEIAKit§kit4250BioCheckBC-1121CK-MBEIAKit*kit6680BioCheckBC-1129MPOEIAKit*kit8480BioCheckBC-1111EstradiolEIAKit§kit4250BioCheckBC-1113ProgesteroneEIAKit§kit4250BioCheckBC-1115TestosteroneEIAKit§kit4250BioCheck我们公司Zda优势是强大的采购，1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，2：货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，3：提供加急服务，一般1-2周到货，超过时限加急费全免4：价格公道，绝大部分价格有优势，当然不能保证1**%产品都有价格优势，5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大交大复旦，中山等100多所大学，ROCHE，阿斯利康，国药，fisher等500多家公司6：我们还是santa,AdvancedBiotechnologiesInc;AthensResearch&Technology,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.FormuMaxScientific,Inc;Genebridege;GlycotopeBiotechnologyGmbH;iduron;InnovativeResearchofAmerica；Ludger；neuroprobe;omicronbio;Polysciences;prospecbi;QA-BIO；quickzyme；RESEARCHDIETS,INC；sterlitech；sysy；TriLinkBioTechnologies,Inc；BIOCHECKINC-biochem;zyagen;......几十家国外公司授权代理7：我们还从事invitrogen，qiagen;abcam;sigma;neb;roche;merck;rnd;BD;GE;pierce;BioLegend....等批发[详细]

  2018-09-29 10:02

  产品样册

  |

  • 
  • 
  • 
  • 

• Veriti 96 PCR

  Veriti 96 PCR[详细]

  2024-09-18 18:10

  课件

  |

  • 
  • 
  • 
  • 

• 96通量移液工作站

  96移液工作站产品特点： 1、96通道同时移液，有效消除板内加样反应时间差； 2、吸喷高度自动控制，保证加样重现性； 3、3种不同量程的移液活塞自由更换，精度控制准确； 4、触摸屏操作清晰易懂，符合常用行为习惯； 5、一吸一喷、一吸多喷、吹打混匀3种标准模式； 6、耗材开放，可使用通用吸头，96孔板均可使用； 7、规格：宽260mm 长350mm 高511mm[详细]

  2024-09-13 02:41

  产品样册

  |

  • 
  • 
  • 
  • 

• 96通量移液工作站

  96通量移液工作站可定制[详细]

  2024-09-11 18:52

  产品样册

  |

  • 
  • 
  • 
  • 

• 国家ZD新产品

  国家ZD新产品[详细]

  2003-04-07 00:00

  报价单

  |

  • 
  • 
  • 
  • 

• 欧司朗LED新产品

  欧司朗2014年新产品。[详细]

  2018-10-30 10:00

  产品样册

  |

  • 
  • 
  • 
  • 

• socorex移液器新产品

  瑞士SOCOREX成立于1940年，是从事高精密液体操作产品的研发，生产及经营的专业厂家。配备完善的技术支持，维修和校准服务。产品包括：手动，电动移液器，多道移液器，瓶口配液器、移液管控制器、连续分液器、连续注射器，特殊用途移液器及各产品完备的附件系统。可以实现液体计量、定量给料，转移传输、等量分配和液体注射等多项液体操作功能。瑞士SOCOREX公司通过对精密液体操作产品及优质服务的高度关注，通过了ISO9001和ISO13485质量管理体系认证。所有产品通过ISO8655，GLP，GMP和NCCLS认证文件，同时所有产品出厂前进行严格QC认证，每个产品都有单独出具的报告文件。SOCOREX每个精密仪器具备有其自身的序列号并通过了严格的品控测试，独立的第三方认证。“一步式解决方案”时SOCOREX服务ZX的格言。依据ISO17025标准，SOCOREX服务ZX提供了性的维修和校准程序。[详细]

  2024-10-10 02:50

  产品样册

  |

  • 
  • 
  • 
  • 

• Eppendorf epMotion 96 Flex 96 道智能移液系统

  为了更好地提升实验效率，Eppendorf epMotion 96 Flex 应运而生。这款专为精度和效率设计的全新 96 通道移液系统，不仅能够大幅提升移液操作速度，显著提高实验通量，还能确保每次移[详细]

  2024-09-14 13:52

  产品样册

  |

  • 
  • 
  • 
  • 

• CAPP 96移液器样本

  CAPP 96移液器样本[详细]

  2024-09-29 11:31

  应用文章

  |

  • 
  • 
  • 
  • 

• FRITSCH加强型新产品简介

  FRITSCH加强型新产品简介[详细]

  2016-05-02 00:00

  安装说明

  |

  • 
  • 
  • 
  •