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pCMV-Tag 2A载体说明书

本文由 上海起发实验试剂有限公司 整理汇编

2018-09-29 10:02 1692阅读次数

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型号载体名称出品公司载体用途价格VPS0039pCMV-Tag2AStratagene哺乳动物表达载体800产品参数:KeyFunction:ConstitutiveExpressionMammalianSelection:Neo,G418Promoter:CMVCloningMethod:RestrictionEnzyme/MCSConstitutiveorInducibleSystem:Constitutive载体抗性:卡那霉素(Kanamycin)载体描述:Epitopetagsprovideamethodtolocalizegeneproductsinlivingcells,identifyassociatedproteins,trackthemovementoftaggedproteinswithinacell,andcharacterizenewgeneswithoutcreatingprotein-specificantibodies.ThepCMV-Tag1vectorcontainsboththesyntheticFLAGepitopellllandthec-mycepitopefromthehumanc-mycgene.ThepCMV-Tag1vectorallowsproductionoffusionproteinsinavarietyofconformations.ThepCMV-Tag2-5vectorsalloweitherc-mycorFLAGepitopetaggingateithertheC-orN-terminus.EachpCMV-Tag2-5vectorissuppliedwithallthreereadingframesforeasycloningandexpression.ThesmallsizeoftheFLAG(eightaminoacidslong)andc-mycepitopetags(tenaminoacidslong)decreasesthepossibilityofinterferencewiththetaggedprotein.TheFLAGandc-mycepitopesarerecognizedbytheanti-c-mycandanti-FLAGantibodies,whichcanbeusedtocharacterizethetargetprotein.ThepCMV-Tagvectorsallowhigh-levelexpressionoftaggedproteinsinmammaliancells.Expressionisdrivenbythehumancytomegalovirus(CMV)immediateearlypromoter.TheCMVpromoterprovidesconstitutiveexpressionofclonedgenesinawidevarietyofcelllines.ThetranslationalstartsequenceusedinthepCMV-Tag1,pCMV-Tag2andpCMV-Tag3vectorsisa10-baseKozakconsensussequenceofCGG(A/G)CCATGG.pCMV-Tag4andpCMV-Tag5vectorsdonotcontainatranslationstartsequence.StablecloneselectionismadepossiblewithG418bythepresenceoftheneomycin-resistancegene.ThepCMV-Tagvectorsareonly4.3kb,allowingeasycloningandvectormanipulations.Thissmallsizeisduetothesingleantibioticselectionmarkerusedbybothprokaryoticandmammaliancells.Theneomycin-resistancegeneprovidesstableselectioninmammaliancellswithG418drivenbytheSV40promoterandkanamycinselectioninE.colicellsdrivenbythebla(-lactamase)promoter.质粒图谱:上海起福生物科技有限公司联系人:杨建辉400电话:4006551678办公电话:021-50724187传真:021-50724961*8006手机:15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址:上海市浦东新区绣川路561号1102室

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