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Promotion of In Vitro Chondrogenesis of Mesenchymal Stem Cells Using In Situ Hyaluronic Hydrogel Functionalized with Rod-Like Viral Nanoparticles
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2024-12-09 09:49 657阅读次数
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Promotion of In Vitro Chondrogenesis of Mesenchymal Stem Cells Using In Situ Hyaluronic Hydrogel Fun
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Promotion of In Vitro Chondrogenesis of Mesenchymal Stem Cells Using In Situ Hyaluronic Hydrogel Functionalized with Rod-Like Viral Nanoparticles Promotion of In Vitro Chondrogenesis of Mesenchymal Stem Cells Using In Situ Hyaluronic Hydrogel Fun[详细]
2024-12-09 09:49
标准
Influence of Cross-Linkers on the in Vitro Chondrogenesis of Mesenchymal Stem Cells in Hyaluronic Acid Hydrogels Influence of Cross-Linkers on the in Vitro Chondrogenesis of Mesenchymal Stem Cells in Hyaluronic Ac[详细]
2024-12-09 10:27
标准
Preparation, in vitro and in vivo evaluation of polymeric nanoparticles bas hyaluronic acid-poly(but Herein,wedescribethepreparationofatargetedcellulardeliverysystemformorinhydrate(MH),-molecular-weighthyaluronicacid-poly(butylcyanoacrylate)(HA-PBCA)blockcopolymer.InordthetherapeuticeffectofMH,D-alpha-tocopherylpolyethyleneglycol1000succinate(TPGS)wasHA-PBCAduringthepreparationprocess.TheMH-loadedHA-PBCA“plain”nanoparticle(MH-PPBCA/TPGS“mixed”nanoparticles(MH-MNs)wereconcomitantlycharacterizedintermsofloadparticlesize,zetapotential,criticalaggregationconcentration,andmorphology.TheobtainedMHMNsexhibitedasphericalmorphologywithanegativezetapotentialandaparticlesizelessthan2favorablefordrugtargeting.Remarkably,theadditionofTPGSresultedinabout1.6-foldincreaseloading.TheinvitrocellviabilityexperimentrevealedthatMH-MNsenhancedthecytotoxicityofcellscomparedwithMHsolutionandMH-PNs.Furthermore,blankMNscontainingTPGSexhibitcytotoxiceffectsagainstcancercellswithoutdiminishingtheviabilityofnormalcells.Inaddition,uptakestudyindicatedthatMNsresultedin2.28-foldhighercellularuptakethanthatofPNs,inA5CD44receptorcompetitiveinhibitionandtheinternalizationpathwaystudiessuggestedthattheintmechanismofthenanoparticleswasmediatedmainlybytheCD44receptorsthroughaclathrin-dependocyticpathway.Moreimportantly,MH-MNsexhibitedahigherinvivoantitumorpotencyandtumorcellapoptosisthandidMH-PNs,followingintravenousadministrationtoS180tumor-bearinOverall,theresultsimplythatthedevelopednanoparticlesarepromisingvehiclesforthetargeteddlipophilicanticancerdrugs.Keywords:anti-tumoreffect,hyaluronicacid,TPGS,morinhydrate,nanoparticles[详细]
2018-08-31 10:11
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2018-08-31 10:11
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马病毒性流产抗体（Viral Abortion-Ab）说明书 www.biokanu.com马病毒性流产抗体(ViralAbortion-Ab)酶联免疫分析（ELISA）试剂盒使用说明书本试剂仅供研究使用目的：本试剂盒用于测定马血清，血浆及相关液体样本中病毒性流产抗体(ViralAbortion-Ab)的含量。实验原理：本试剂盒应用双抗原夹心法测定标本中马病毒性流产抗体(ViralAbortion-Ab)水平。用纯化的马病毒性流产(ViralAbortion)抗原包被微孔板，制成固相抗原，往包被抗原的微孔中加入病毒性流产抗体(ViralAbortion-Ab)，再与HRP标记的病毒性流产(ViralAbortion)抗原结合，形成抗原-抗体-酶标抗原复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成Z终的黄色。颜色的深浅和样品中的病毒性流产抗体(ViralAbortion-Ab)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中马病毒性流产抗体(ViralAbortion-Ab)的浓度。试剂盒组成：试剂盒组成48孔配置96孔配置保存说明书1份1份封板膜2片（48）2片（96）密封袋1个1个酶标包被板1×481×962-8℃保存标准品：90pg/ml0.5ml×1瓶0.5ml×1瓶2-8℃保存标准品稀释液1.5ml×1瓶1.5ml×1瓶2-8℃保存酶标试剂3ml×1瓶6ml×1瓶2-8℃保存样品稀释液3ml×1瓶6ml×1瓶2-8℃保存显色剂A液3ml×1瓶6ml×1瓶2-8℃保存显色剂B液3ml×1瓶6ml×1瓶2-8℃保存终止液3ml×1瓶6ml×1瓶2-8℃保存浓缩洗涤液（20ml×20倍）×1瓶（20ml×30倍）×1瓶2-8℃保存样本处理及要求：1.血清：室温血液自然凝固10-20分钟，离心20分钟左右（2000-3000转/分）。仔细收集上清，保存过程中如出现沉淀，应再次离心。2.血浆：应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂，混合10-20分钟后，离心20分钟左右（2000-3000转/分）。仔细收集上清，保存过程中如有沉淀形成，应该再次离心。3.尿液：用无菌管收集，离心20分钟左右（2000-3000转/分）。仔细收集上清，保存过程中如有沉淀形成，应再次离心。胸腹水、脑脊液参照实行。4.细胞培养上清：检测分泌性的成份时，用无菌管收集。离心20分钟左右（2000-3000转/分）。仔细收集上清。检测细胞内的成份时，用PBS（PH7.2-7.4）稀释细胞悬液，细胞浓度达到100万/ml左右。通过反复冻融，以使细胞破坏并放出细胞内成份。离心20分钟左右（2000-3000转/分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。5.组织标本：切割标本后，称取重量。加入一定量的PBS，PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS（PH7.4），用手工或匀浆器将标本匀浆充分。离心20分钟左右（2000-3000转/分）。仔细收集上清。分装后一份待检测，其余冷冻备用。6.标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融.7.不能检测含NaN3的样品，因NaN3YZ辣根过氧化物酶的（HRP）活性。操作步骤1.标准品的稀释与加样：在酶标包被板上设标准品孔10孔，在**、第二孔中分别加标准品100μl，然后在**、第二孔中加标准品稀释液50μl，混匀；然后从**孔、第二孔中各取100μl分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液50μl，混匀；然后在第三孔和第四孔中先各取50μl弃掉，再各取50μl分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液50ul，混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液50μl，混匀后从第七、第八孔中分别取50μl加到第九、第十孔中，再在第九第十孔分别加标准品稀释液50μl，混匀后从第九第十孔中各取50μl弃掉。（稀释后各孔加样量都为50μl，浓度分别为60pg/ml，40pg/ml，20pg/ml，10pg/ml，5pg/ml）。2.加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、待测样品孔。在酶标包被板上待测样品孔中先加样品稀释液40μl，然后再加待测样品10μl（样品Z终稀释度为5倍）。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀。3.温育：用封板膜封板后置37℃温育30分钟。4.配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用。5.洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置30秒后弃去，如此重复5次，拍干。6.加酶：每孔加入酶标试剂50μl，空白孔除外。7.温育：操作同3。8.洗涤：操作同5。9.显色：每孔先加入显色剂A50μl，再加入显色剂B50μl，轻轻震荡混匀，37℃避光显色15分钟.10.终止：每孔加终止液50μl，终止反应（此时蓝色立转黄色）。11.测定：以空白空调零，450nm波长依序测量各孔的吸光度（OD值）。测定应在加终止液后15分钟以内进行。注意事项：1．试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存。2．浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果。3．各步加样均应使用加样器，并经常校对其准确性，以避免试验误差。一次加样时间**控制在5分钟内，如标本数量多，推荐使用排枪加样。4．请每次测定的同时做标准曲线，**做复孔。如标本中待测物质含量过高（样本OD值大于标准品孔**孔的OD值），请先用样品稀释液稀释一定倍数（n倍）后再测定，计算时请Z后乘以总稀释倍数（×n×5）。5．封板膜只限一次性使用，以避免交叉污染。6．底物请避光保存。7．严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准.8．所有样品，洗涤液和各种废弃物都应按传染物处理。9．本试剂不同批号组分不得混用。10.如与英文说明书有异，以英文说明书为准。计算：以标准物的浓度为横坐标，OD值为纵坐标，在坐标纸上绘出标准曲线，根据样品的OD值由标准曲线查出相应的浓度；再乘以稀释倍数；或用标准物的浓度与OD值计算出标准曲线的直线回归方程式，将样品的OD值代入方程式，计算出样品浓度，再乘以稀释倍数，即为样品的实际浓度。（此图仅供参考）试剂盒性能：1.样品线性回归与预期浓度相关系数R值为0.92以上。2.批内与批见应分别小于9%和15%检测范围：2pg/ml-80pg/ml保存条件及有效期：1.试剂盒保存：；2-8℃。2．有效期：6个月[详细]
2018-09-22 10:00
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Promotion of In Vitro Chondrogenesis of Mesenchymal Stem Cells Using In Situ Hyaluronic Hydrogel Functionalized with Rod-Like Viral Nanoparticles
马病毒性流产抗体（Viral Abortion-Ab）说明书
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