pEF1/myc-His C载体说明书

pEF1/myc-His C载体说明书

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型号载体名称出品公司载体用途价格VPI0028pEF1/myc-HisCInvitrogen哺乳动物表达载体800产品参数:KeyFunction：ConstitutiveExpressionMammalianSelection：Neo，G418Promoter：EF-1αVectorType：pEFCloningMethod：RestrictionEnzyme/MCSProteinTagorFusion：c-MycEpitopeTag，HisTag（6xHis）ConstitutiveorInducibleSystem：Constitutive载体抗性:氨苄青霉素（Ampicillin）载体描述:pEF1/myc-HisA,B,andCare6.2kbvectorsderivedfrompcDNA3.1/myc-Hisanddesignedforoverproductionofrecombinantproteinsinmammaliancelllines.Featuresofthevectorsallowpurificationanddetectionofexpressedproteins.High-levelstableandtransientexpressioncanbecarriedoutinmostmammaliancells.Thevectorscontainthefollowingelements:Humanelongationfactor1?-subunit（hEF-1α）promoterforhigh-levelexpressionacrossabroadrangeofspeciesandcelltypes（Goldmanetal.,1996;MizushimaandNagata,1990）.Threereadingframestofacilitatein-framecloningwithaC-terminaltagencodingthemyc（c-myc）epitopeandapolyhistidinemetal-bindingpeptide.Neomycinresistancegeneforselectionofstablecelllines.EpisomalreplicationincelllinesthatarelatentlyinfectedwithSV40orthatexpresstheSV40largeTantigen（e.g.,COS7）.Thecontrolplasmid,pEF1/myc-His/lacZ,isincludedforuseasapositivecontrolfortransfection,expression,anddetectioninthecelllineofchoice.质粒图谱:

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pEF1/myc-His C载体说明书

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pVAX1载体说明书

pVAX1型号载体名称出品公司载体用途VPI0038pVAX1Invitrogen哺乳动物表达载体产品参数:MammalianSelection：-SequencingPrimer：T7FwdSequencingPrimerSequence：5'd[TAATACGACTCACTATAGGG]3'载体抗性:卡那霉素（Kanamycin）载体描述:pVAX1是在载体pcDNA3.1的基础上改建而成的一种新型真核表达载体，大小为3.0kb。该质粒用卡那霉素抗性筛选基因替代pcDNA3.1中的氨卞霉素抗性筛选基因，Z*程度地减少抗性筛选基因和人类基因组发生重组的可能性。pVAX1含有多个克隆酶切位点允许同时克隆多个大片段的目的基因，强启动子pCMV和BGHpolyA信号可以在哺乳动物细胞中高水平表达重组蛋白。pVAX1又是一种非融合载体，要求插入序列中时必须包含一段KozaK的翻译起始序列和一个起始密码子（ATG）以引导正确的翻译，典型的KozaK共有序列为“ANNATGG”，其-3位的A和+4位的G对正确插入甚为关键，在插入序列中还需包括一个终止密码子以正确终止基因表达。pVAX1是由美国食品和药品管理委员会（FDA）推荐的**可以应用于人体实验的载体质粒。迄今为止，关于pVAX1的研究已涉及动物和临床试验。质粒图谱:上海起福生物科技有限公司联系人：杨建辉400电话：4006551678办公电话：021-50724187传真：021-50724961*8006手机：15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址：上海市浦东新区绣川路561号1102室[详细]
2018-09-29 10:02
产品样册
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pBC1载体说明书

pBC1型号载体名称出品公司载体用途VCI0066pBC1Invitrogen重组蛋白乳汁表达系统GeneralDescriptionDNApBC1Entiremoleculelength:21628bp质粒图谱:[详细]
2018-09-29 10:02
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pDisplay载体说明书

pDisplay型号载体名称出品公司载体用途VBI0471pDisplayInvitrogen哺乳动物表面展示系统产品参数:MammalianSelection：Neomycin，G418Host：mammalianN/CTerm：CterminusMCS：SfiI-BglII-XmaI-SmaI-SacII-PstI-SalI-AccI载体抗性:氨苄青霉素（Ampicillin）载体描述:pDisplayisamammalianexpressionvectordesignedtotargetrecombinantproteinstothesurfaceofmammaliancells.Displayedproteinscanbeanalyzedfortheirabilitytointeractwithknownorputativeligands.ProteinsofinterestaretargetedandanchoredtothecellsurfacebycloningthegeneofinterestinframewiththevectorsuniqueN-terminalsecretionsignalandtheC-terminaltransmembraneanchoringdomainofplatelet-derivedgrowthfactorreceptor（PDGFR）.Incontrasttophagedisplayvectorswhichoperateexclusivelyinprokaryoticcells,thepDisplayVectorofferstheadvantageofhavingthedisplayedproteinofinterestprocessedinmammaliancells.Therefore,recombinantproteinsofeukaryoticoriginthatareexpressedfrompDisplaymorecloselyresembletheirnativeform.Thismayfavormoreaccurateligandbindinginteractions.InadditiontotheN-terminalcellsurfacetargetingsignalandC-terminaltransmembraneanchoringdomain,thepDisplayVectorcontainsthefollowingkeyfeatures:T7promoter/primingsiteforinvitrotranscriptionofsenseRNAandforsequencingofinsertsNeomycinresistancemarkerforstableselectioninmammaliancellsusingGeneticinSV40originforreplicationandsimplevectorrescueincelllinesexpressingthelargeTantigen（e.g.,COS-1andCOS-7）AmpicillinresistancegeneforselectioninE.coli质粒图谱:上海起福生物科技有限公司联系人：杨建辉400电话：4006551678办公电话：021-50724187传真：021-50724961*8006手机：15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址：上海市浦东新区绣川路561号1102室[详细]
2018-09-29 10:02
产品样册
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EBY100载体说明书

EBY100型号载体名称出品公司载体用途VNI0473EBY100Invitrogen配套酿酒酵母Contents20gofpYD1,lyophilizedinTE,pH8EBY100glycerolstock,0.5ml2gpYD1Forwardsequencingprimer,lyophilized2gpYD1Reversesequencingprimer,lyophilizedShipping/StorageThepYD1VectorKitisshippedondryice.Uponreceipt:Storetheglycerolstockat-80°CStorethelyophilizedplasmidandprimersat-20°C上海起福生物科技有限公司联系人：杨建辉400电话：4006551678办公电话：021-50724187传真：021-50724961*8006手机：15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址：上海市浦东新区绣川路561号1102室[详细]
2018-09-29 10:02
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该会员其他资料: D12492说明书; CYGNUS F050说明书; HIV P24 ELISA KIT 说明书; research diets D12451说明书; research diets d12450b说明书; omicronbio产品; Smartox-biotech产品; phytotechlab产品; 美天旎macs代理; polysciences产品目录

pEF1/myc-His C载体说明书

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