仪器网(yiqi.com)欢迎您!

| 注册2 登录
网站首页-资讯-专题- 微头条-话题-产品- 品牌库-搜索-供应商- 展会-招标-采购- 社区-知识-技术-资料库-方案-产品库- 视频

资料库

仪器网> 资料库>pGAPZα A载体说明书

pGAPZα A载体说明书

本文由 上海起发实验试剂有限公司 整理汇编

2018-09-29 10:02 1939阅读次数

扫    码    分   享
文档仅可预览首页内容,请下载后查看全文信息!
立即下载
pGAPZαA型号载体名称出品公司载体用途VJI0293pGAPZαAInvitrogen酵母表达载体Theglyceraldehyde-3-phosphatedehydrogenase(GAPDH)enzymeisconstitutivelyexpressedathighlevelsinmanyorganisms,includingPichiapastoris.Thepromoterofthegene(GAP)encodingtheGAPDHproteinhasrecentlybeencharacterizedandshowntoexpressrecombinantproteinstohighlevelsinPichiapastoris,dependingonthecarbonsourceused(Waterhametal.,1997).ThelevelofexpressionseenwiththeGAPpromoter(PGAP)canbeslightlyhigherthanthatobtainedwiththeAOX1promoter.ThepGAPZA,B,andCvectors(2.9kb)andpGAPZαA,B,andC(3.1kb)vectorsusetheGAPpromotertoconstitutivelyexpressrecombinantproteinsinPichiapastoris.ProteinscanbeexpressedasfusionstoaC-terminalpeptidecontainingthemycepitopefordetectionandapolyhistidinetagforpurificationonmetal-chelatingresin(i.e.ProBond).Inaddition,pGAPZαproducesproteinsfusedtoanN-terminalpeptideencodingtheSaccharomycescerevisiaeα-factorsecretionsignal.BothvectorsaresuppliedinthreereadingframestofacilitateinframecloningwiththeC-terminaltagand/ortheN-terminalsecretionsignal.Selectionofthesevectorsisbasedonthedominantselectablemarker,Zeocin,whichisbifunctionalinbothPichiaandE.coli.质粒图谱:上海起福生物科技有限公司联系人:杨建辉400电话:4006551678办公电话:021-50724187传真:021-50724961*8006手机:15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址:上海市浦东新区绣川路561号1102室

参与评论

全部评论(0条)

更多资料

该会员其他资料
D12492说明书
CYGNUS F050说明书
HIV P24 ELISA KIT 说明书
research diets D12451说明书
research diets d12450b说明书
omicronbio产品
Smartox-biotech产品
phytotechlab产品
美天旎macs代理
polysciences产品目录

在线留言

换一张?
取消