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Functionalization of polyamide 6 nanofibers by elect
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2008-09-24 00:00 663阅读次数
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Functionalization of polyamide 6 nanofibers by elect
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Functionalization of polyamide 6 nanofibers by elect
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Time-resolved ion flux measurements in pulsed, elect
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Basic and acidic surface oxides on carbon fiber and their influence on the expected adhesion to polyamide
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finesse 6(532 nm,6 W)
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激光粒度仪6
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人激肽释放酶6(KLK 6)ELISA试剂盒
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vilber 凝胶成像-6
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SFM300应用资料6
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Goat Anti-Pig Interleukin 6
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GoatAnti-PigInterleukin6Storage:2-8°CPackagesize:96determinationsPRINCIPLEOFTHEMETHODTheIL-6kitisasolidphasephasesandwichenzymelinkedimmunosorbentassay(ELISA).Samples,includingstandardsofknownIL-6concentrationsandunknownsarepipettedintothesewells.Duringthefirstincubation,theIL-6antigenandabiotinylatedmonoclonalantibodyspecificforIL-6aresimultaneouslyincubated.Afterwashing,theenzyme(streptavidin-peroxydase)isadded.Afterincubationandwashingtoremovealltheunboundenzyme,asubstratesolutionwhichisactingontheboundenzymeisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofIL-6presentinthesamples.REAGENTSPROVIDEDANDRECONSTTTUTIONREAGENTS(Storeat2-8℃)1×96WELLS0.5×96WELLSRECONSTTTUTION96/48-wellsmicrotiterplates10.5Ready-to-usePlastivcover21Ready-to-useStandard:800pg/ml1Vials(0.6ml)0.5Vials(0.3ml)Seereagentspreparationonpage3Blankcontrol1Vials(1.0ml)1Vials(0.5ml)Ready-to-useStandardDiluent1Vials(4.0ml)1Vials(2.0ml)Ready-to-useBiotinylatedanti-IL-61Vials(6.0ml)1Vials(3.0ml)Ready-to-useStreptavidin-HRP1Vials(8.0ml)1Vials(4.0ml)Ready-to-useWashingBuffer1Vials(20ml)1Vials(10ml)50×concentrateSubstrateA1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSubstrateB1Vials(6.0ml)1Vials(3.0ml)Ready-to-useStoppingSolution1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSampleDiluent1Vials(12ml)1Vials(6.0ml)Ready-to-useMATERIALREQUIREDBUTNOTPROVIDED?Distilledwater?Pipettes:10ul、50ul、100ul、200ul、1000ul。?Vortexmixerandmagneticstirrer.SAFETY?Forresearchuseonly?AvoidanyskincontactwithH2SO4andTMB.Incaseofcontact,washthoroughlywater.?Donoteat,drink,smokeorapplycosmeticswherekitreagentsareused.?Donotpipettebymouth.PROCEDURALNOTES/LAB.QUALITYCONTROL?Whennotinuse,kitcomponents首ldbestoredrefrigeratedorfrozenasindicatedonvialsorbottles.Allreagents首ldbewarmedtoroomtemperaturebeforeuse.Lyophilizedstandards首ldbediscardedafteruse.?Oncethedesirednumberofstripshasbeenremoved,immediatelyresealthebagtoprotecttheremainingstripsfromedterioration.?Coverorcapallreagentswhennotinuse.?Donotmisorinterchangereagentsbetweendifferentlots.?Donotusereagentsbeyondtheexpirationdateofthekit.?Useacleandisposableplasticpipettetipforeachreagent,standard,orspecimenadditioninordertoavoidcross-contamination,forthedispensingofH2SO4andsubstratesolution,avoidpipetteswithmetalparts.?Useacleanplasticcontainertopreparethewashingsolution.?Thoroughlymixthereagentsandsamplesbeforeusebyagitationorswir领.?Allresidualwashingliquidmustbedrainedfromthewellsbyefficientaspirationorbydecantationfollowedbytappingtheplateforcefullyonabsorbentpaper.Neverinsertabsorbentpaperdirectlyintothewells.?TheTMBsolutionislightsensitive.Avoidprolongedexposuretolight,also,avoidcontactoftheTMBsolutionwithmetaltopreventcolourdevelopment.WarningTMBistoxicavoiddirectcontactwithhands.Disposeoffproperly.Ifadarkbluecolourdevelopswithinafewminutesafterpreparation,thisindicatesthattheTMBsolutionhasbeencontaminatedandmustbediscarede.Readabsorbanceswithin1houraftercompletionoftheassay.?Whenpipettingreagents,maintainaconsistentorderofadditionfromwell-to-well.Thiswillensureequalincubationtimesforallwells.?Respectincubationtimesdescribedintheassayprocedure.SPECIMENCOLLECTION\PROCESSINGANDSTORAGE?Serum---Avoidanyinintentionalstimulationofthecellsbytheprocedure.Usepyrogen\endotoxinfreecollectingtubes.Serum首ldberemovedrapidlyandcarefullyfromtheredcellsafterclothing.Forthat,afterclothing,centrifugeatapproximately1000×gfor10minandremoveserum.?Plasma---EDTA\citrateandheparinplasmacanbeassayed.Spinsamplesat1000×gfor30minremoveparticulates.Harvestplasma.?Cellculturesupernatants---Removeparticulatesandaggregatesbyspinningatapproximately1000×gfor10min.?Storage---Ifnotanalyzedshortlyaftercollection,samples首ldbealiquoted(250-500ul)toavoidfreeze-thawcyclesandstoredfrozenat-70℃.Avoidmultiplefreeze-thawcyclesoffrozenspecimens.Whenpossible,avoiduseofbadlyhemolyzedorlipemicsera.Iflargeamountsofparticlesarepresent,this首ldberemovedpriortoassaybycentrifugationorfiltration.?Recommendation---Donotthawbyheatingat37℃or56℃.Thawatroomtemperatureandmakesurethatsampleiscompletelythawedandhomogenousbeforeassaying.PREPARATIONOFREAGENTS?Standards:Standardhavetobereconstituledwiththevolumeofstandardbufferdiluentindicatedonthevial.Thisreconstitutionproducesastocksolutionof800pg/mlIL-6.Allowstandardtostandfor5?minuteswithgentleswir领priortomakingdilutions.Serialdilutionsofstandardmustbemadebeforeeachassysandcannotbestored.800pg/ml(6Standard)Originaldensity50ul。400pg/ml(5Standard)100ul6Standard+100uldiludent200pg/ml(4Standard)100ul5Standard+100uldiludent100pg/ml(3Standard)100ul4Standard+100uldiludent50pg/ml(2Standard)100ul3Standard+100uldiludent25pg/ml(1Standard)100ul2Standard+100uldiludent0pg/mlBlankControl50ul。?Washingbuffer50×concentrate:Dilute50timesindistilledwater.ASSAYMETHOD?Beforeuse,mixallreagentsthoroughlywithoutmakingfoam.?Determinethenumberofmicrowellstripsrequiredtotestthedesirednumberofsamples,plusappropriatenumberofwellsneededforrunningblanksstandards.Eachsample,standardandblank首ldbeassayedinduplicate.Removesufficientmicrowellstripsfromthepouch.?Add50ulofstandarddiluenttostandardwellsB1,B2,C1,C2,D1,D2,E1,E2,F1,F2.Reconstitutestandardvialwiththeappropriatevolumeasdescribedinthechapterreagentspreparation.Preparation.Pipet100ulofstandardintowellsA1andA2(seeplateschemebelow).Transfer50ulfromA1andA2toB1andB2wells.Mixthecontentsbyrepeatedaspirationsandejections.Takecarenottoscratchtheinnersurfaceofmicrowells.RepatthisprocedurefromthewellsB1,B2towellsC1,C2andfromwellsC1,C2toD1,D2andsooncreatingtwoparallelrowsofIL-6standarddilutionsranging,Add50ulofstandarddiluenttotheblandwells.?Dilutesamples1:1distribing50ulofsampleinto50ulofdilluent,Add50ulofdilutedsampletowells..?Add50ulofdilutedbiotinylatedanti-IL-6toallwells.?Coverwithaplatevoverandincubatefor1hourat37℃.?Removethecoverandwashtheplateasfollows:⑴aspiratetheliquidfromeachwell,⑵dispensse0.3mlofwashingsolutionintoeachwell.⑶Aspirateagainthecontetofeachwellafter0.5minute.⑷Repeatsteps⑵and⑶threetimes.?Distribute60ulofstreptavidin-HRPsolutiontoallwells,includingblankwells.?Coverandincubate30minat37℃.?Removethecoverandemptywells,Washmicrowellstripsaccordingtostep,Proceedimmediatelytothenextstep.?Add50ulSubstrateAandSubstrateBtoeachwell。Incubatefor10minat37℃。?Theenzyme-substratereactionisstoppedbyquicklypipetting50ulofH2SO4.stopreagentintoeachwell,includingtheblankwells,tocompletelyanduniformlyinactivatetheenzyme.ResultsmustberedimmediatelyaftertheadditionofH2SO4.?Readabsorbanceofeachwellonaspectrophotometerusing450nmastheprimarywavelengthandoptionally620nm(610nmto650nmisacceptable)asthereferencewavelength.SUGGESTEDPLATESCHEMEStandardconcentrations(pg/ml)A800800samplesamplesamplesamplesamplesamplesamplesamplesamplesampleB400400samplesamplesamplesamplesamplesamplesamplesamplesamplesampleC200200samplesamplesamplesamplesamplesamplesamplesamplesamplesampleD100100samplesamplesamplesamplesamplesamplesamplesamplesamplesampleE5050samplesamplesamplesamplesamplesamplesamplesamplesamplesampleF2525samplesamplesamplesamplesamplesamplesamplesamplesamplesampleG00samplesamplesamplesamplesamplesamplesamplesamplesamplesampleHsamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesampleLIMITATIONSOFTHEPROCEDUREDonotextrapolatethestandardcurvebeyondthemaxstandardcurvepoint.Thedose-responseisnon-linearinthisregionandgoodaccruacyisdifficulttoobtain.CALCULATIONOFRESULTSTheminimumdetectableconcentrationinthisassayisestimatedtobe1.0pg/ml[详细]
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KK8230KAPALTP文库构建(含文库扩增)试剂盒-Illumina10个文库2500KK8231KAPALTP文库构建(不含文库扩增)试剂盒-Illumina10个文库2400KK8232KAPALTP文库构建(含文库扩增)试剂盒-Illumina50个文库11000KK8233KAPALTP文库构建(不含文库扩增)试剂盒-Illumina50个文库10500KK8234KAPAHTP文库构建(含文库扩增)试剂盒-Illumina96个文库18000KK8235KAPAHTP文库构建(不含文库扩增)试剂盒-Illumina96个文库17000KK8260KAPATruSeqAdapterKit,(Illumina,20μleach)144rxn14000KK2620KAPALibraryAmplificationKitwithPrimers(50x50lreactions)50rxn1800KK2621KAPALibraryAmplificationKitwithPrimers(250x50lreactions)250rxn8000KK2623KAPALibraryAmplificationPrimerKit(250x50μlrxns)250rxn1500KK8400KAPAStrandedRNA-SeqLibraryPreparationKit(Illumina,24reactions)24rxn12200KK8401KAPAStrandedRNA-SeqLibraryPreparationKit(96reactions)96rxn38000KK8420KAPAStrandedmRNA-SeqLibraryPreparationKit(24reactions)24rxn14000KK8421KAPAStrandedmRNA-SeqLibraryPreparationKit(96reactions)96rxn44000KK8500KAPAHyperPrepKit(8reactions)8rxn3100KK8501KAPAHyperPrepKit,noamplification(8reactions)8rxn2970KK8502KAPAHyperPrepKit(24reactions)24rxn8100KK8503KAPAHyperPrepKit,noamplification(24reactions)24rxn7830KK8504KAPAHyperPrepKit(96reactions)96rxn28100KK8505KAPAHyperPrepKit,noamplification(96reactions)96rxn27000KK8300IonTorrentLibraryPreparationKit(8reactions)8rxn2520KK8301IonTorrentLibraryPreparationKit(48reactions)48rxn13860KK8310IonTorrentLibraryPreparationKit,noamplification(8reactions)8rxn2280KK8311IonTorrentLibraryPreparationKit,noamplification(48reactions)48rxn12420KK8330AdapterKit,non-Barcoded(8reactionsIonTorrent8rxn1200KK8331AdapterKit,Barcodes1-8(48reactions)IonTorrent48rxn7500KK8332AdapterKit,Barcodes9-16(48reactions)IonTorrent48rxn7500KK8333AdapterKit,Barcodes17-24(48reactions)IonTorrent48rxn7500二代测序文库定量:标准品+引物预混物KK4800DNAQuantKitRoche454FLXEach6000KK4801PrimerPremixRoche454FLXEach3000KK4802DNAQuantKitRoche454TitaniumEach6000KK4803PrimerPremixRoche454TitaniumEach3000KK4804DNAQuantKitIlluminaGenomeAnalyzerEach6000KK4805PrimerPremixIlluminaGenomeAnalyzerEach3000KK4806DNAQuantKitABISOLiDEach6000KK4807PrimerPremixABISOLiDEach3000KK4808DNAQuantKitIlluminaGARevisedPrimersEach6000KK4809PrimerPremixIlluminaGARevisedPrimersEach3000KK4812DNAQuantKit-IonTorrentEach6000KK4813DNAQuantificationPrimerPremix-IonTorrentEach3000二代测序文库定量:标准品KK4900LibraryQuantificationDNAStandards(454FLX/Lib-A)6x80l4000KK4902LibraryQuantificationDNAStandards(SOLiD)6x80l4000KK4903LibraryQuantificationDNAStandards(Illumina)6x80l4000KK4904LibraryQuantificationDNAStandards(IonTorrent)6x80l4000二代测序Z终文库定量产品KK4820LibraryQuantKit,Roche454FLX-SYBRFastUniversalEach7000KK4821LibraryQuantKit,Roche454Titanium-SYBRFastUniversalEach7000KK4824LibraryQuantKit,IlluminaGArevisedprimers-SYBRFastUniversalEach7000KK4823LibraryQuantKit,ABISOLiD-SYBRFastUniversalEach7000KK4830LibraryQuantKit,Roche454FLX-SYBRFastABIPrismEach7000KK4831LibraryQuantKit,Roche454Titanium-SYBRFastABIPrismEach7000KK4835LibraryQuantKit,IlluminaGArevisedprimers-SYBRFastABIPrismEach7000KK4833LibraryQuantKit,ABISOLiD-SYBRFastABIPrismEach7000KK4840LibraryQuantKit,Roche454FLX-SYBRFastBio-RadiCyclerEach7000KK4841LibraryQuantKit,Roche454Titanium-SYBRFastBio-RadiCyclerEach7000KK4844LibraryQuantKit,IlluminaGArevisedprimers-SYBRFastBio-RadiCyclerEach7000KK4843LibraryQuantKit,ABISOLiD-SYBRFastBio-RadiCyclerEach7000KK4850LibraryQuantKit,Roche454FLX-SYBRFastRocheLightCycler480Each7000KK4851LibraryQuantKit,Roche454Titanium-SYBRFastRocheLightCycler480Each7000KK4854LibraryQuantKit,IlluminaGArevisedprimers-SYBRFastRocheLightCycler480Each7000KK4853LibraryQuantKit,ABISOLiD-SYBRFastRocheLightCycler480Each7000KK4834LibraryQuantKit,IlluminaGAABIPrismCustomKit-SangerEach7000KK4827LibraryQuantKit,IonTorrentPGMuniversalEach7000KK4838LibraryQuantKit,IonTorrentPGMABIPrismEach7000KK4847LibraryQuantKit,IonTorrentPGMBio-RadiCyclerEach7000KK4857LibraryQuantKit,IonTorrentPGMRocheLightCycler480Each7000人类基因组专用定量Kit(文库构建之前用,主要检测提取的DNA量)KK4960hgDNAQuant&QCkitwithSYBRUniversalqPCRMastermixEach8000KK4961hgDNAQuant&QCkitwithSYBRABIPrismqPCRMastermixEach8000KK4962hgDNAQuant&QCkitwithSYBRBio-RadqPCRMastermixEach8000KK4963hgDNAQuant&QCkitwithSYBRLightCycler480qPCRMastermixEach8000KK4964hgDNAQuant&QCDNAStandardsKitEach6000KK4965hgDNAQuant&QCand41bpPrimerKitEach1500KK4966hgDNAQuant&QCand129bpPrimerKitEach1500KK4967hgDNAQuant&QCand305bpPrimerKitEach1500 KK4969hgDNAQuant&QCKitwithROXLowqPCRMastermixEach8000普通Taq酶及T4连接酶产品归类ProductCodeProductCategoryQuantity Taq相关产品KK1014Taq酶250units380KK1015Taq酶500units700BK1000Taq酶2500units2500BK1002Taq酶5000units3800KK1008Taq酶(含dNTPs)250units450KK1016Taq酶(含dNTPs)500units800BK1001Taq酶(含dNTPs)2500units3500BK1003Taq酶(含dNTPs)5000units5500KK1006Taq酶ReadyMix250×50l反应900KK1020Taq酶Bufferw/loadingdye250units380KK1022Taq酶Bufferw/loadingdye500units700BK1004Taq酶Bufferw/loadingdye2500units2500BK1006Taq酶Bufferw/loadingdye5000units3800KK1021Taq酶Bufferw/loadingdye(含dNTPs)250units450KK1023Taq酶Bufferw/loadingdye(含dNTPs)500units800BK1005Taq酶Bufferw/loadingdye(含dNTPs)2500units3500BK1007Taq酶Bufferw/loadingdye(含dNTPs)5000units5500KK1024Taq酶ReadyMixw/loadingdye250×50l反应900KK1508热启动Taq酶250units800KK1510热启动Taq酶500units1200KK1513热启动Taq酶2500units5000KK1509热启动Taq酶(含dNTPs)250units900KK1511热启动Taq酶(含dNTPs)500units1500KK1512热启动Taq酶withoutbuffers2500units5000T4DNA连接酶KK6010T4DNALigase,5units/L(WeissUnits)250units600KK6011T4DNALigase,5units/L(WeissUnits)1250units2500KK6110T4DNALigase,RapidLigationKit50rxn1400KK6111T4DNALigase,RapidLigationKit150rxn3800上海起发实验试剂有限公司实验试剂一站式采购服务商1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。2:产品种类齐全,经营超过700多个品牌,基本涵盖所有生物实验试剂耗材。3:提供加急服务,货品一般1-2周到货。4:富有竞争力的价格优势,绝大部分价格有优势。5:多年积累良好的信誉,大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校,ROCHE,阿斯利康、国药、fisher等知名药企。6:我们还是Santa,AdvancedBiotechnologiesInc,AthensResearch&Technology,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.FormuMaxScientific,Inc,Genebridege,GlycotopeBiotechnologyGmbH;iduron,InnovativeResearchofAmerica,Ludger,neuroprobe,omicronbio,Polysciences,prospecbi,QA-BIO,quickzyme,RESEARCHDIETS,INC,sterlitech;sysy,TriLinkBioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司授权代理。7:我们还是invitrogen,qiagen,RNDSYSTEMSam,sigma;neb,roche,merck,rnd,BD,GE,pierce,BioLegend等知名批发,欢迎合作。[详细]
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2024-09-29 15:05
产品样册
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豚鼠白细胞介素-6 说明书
- 豚鼠白细胞介素-6说明书本试剂仅供研究使用标本:血清一、试剂组成1、精密度微孔板MicrotestPlates96wells2、酶标偶合液EnzymeConjugate12.0ml3、标准品Standard5x1.0ml4、呈色剂A、SubstrateA6.0ml5、呈色剂B、SubstrateB6.0ml6、终止液StopSolution6.0ml7、浓缩洗涤液(1:20)RinsingBuffer60.0ml8、5倍样品稀释液dilution15.0m二、注意事项1.此试剂为体外检测试剂,效期内使用,试剂应视为传染物,不同总批号的试剂不能混用。2.使用前应将盒内各试剂取出,室温放置至少30分钟。3.保存于2-8℃,请勿冷冻,有效期请见盒内标示。4.浓缩洗涤液出现结晶后,请于37℃孵育15分钟豚鼠白细胞介素-6说明书三、操作步骤1.每孔分别加入已稀释的样品、标准品各100ul。2.将板置于37℃温育30分钟。3.甩尽板中液体,用应用洗涤液进行洗涤,每次停留30秒,在纸上拍干。重复操作5次。4.每孔加入酶标偶合液100ul。5.将板置于37℃温育30分钟。6.甩尽板中液体,用应用洗涤液进行洗涤,每次停留30秒,在纸上拍干。重复操作5次。7.每孔加底物A、底物B各50ul,置37℃恒温箱反应15分钟。8.每孔加终止液50ul,于450nm波长读O.D.值。豚鼠白细胞介素-6说明书四、结果判断仪器值:于波长450nm的酶标仪上读取各孔的OD值。检测值范围:0400pg/ml敏感度:1.0pg/ml[详细]
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2024-09-29 10:54
产品样册
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DPS气相色谱仪应用6
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2024-09-29 05:37
期刊论文
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药典实验(6)-桔梗
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2024-09-29 04:10
选购指南
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有机氯农药解决方案 6
- 有机氯农药解决方案 6[详细]
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2014-12-26 00:00
实验操作
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