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Design, synthesis and anti-tuberculosis activity of
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Design, synthesis and anti-tuberculosis activity of
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Design, synthesis and anti-tuberculosis activity of- Design, synthesis and anti-tuberculosis activity of[详细]
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2024-09-29 03:25
专利
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- Synthesis and xanthine oxidase inhibitory activity o
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2024-09-28 01:18
应用文章
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- Cytochrome Oxidase Activity
- CytochromeOxidaseActivityColorimetricAssayKitrev.04/13(Catalog#K287-100;100assays;Storeat-20°C)I.Introduction:CytochromecOxidase(EC1.9.3.1)orComplexIVisthefourthcomplexoftheElectronTransportChainlocatedinthemitochondrial(orbacterial)membrane.Itprovidesenergytothecellbycoup领electrontransportthroughtheCytochromecchainwiththeprocessofoxidativephosphorylation.ComplexIVcontains13differentsubunitsencodedbybothmitochondrialDNAandnuclearDNA.ItreceivesanelectronfromeachofthefourCytochromecmolecules,andtransfersittooneoxygenmolecule,convertingitintotwomoleculesofwater.Inthisprocess,italsobindstofourprotonmoleculesandtranslocatesthemacrossthemembranetoestablishelectrochemicalgradient,whichisutilizedforthesynthesisofATP.CytochromeOxidaseActivityAssayKitissimple,fastandhigh-throughputadaptable.Thisassaykitcanbeusedforpurifiedmitochondriaortissueextractscontainingmitochondria.TheactivityoftheenzymeisdeterminedcolorimetricallybyfollowingtheoxidationofreducedCytochromecasanabsorbancedecreaseat550nm.Theoverallreactionisasfollows:II.Application:FastandsimplemeasurementofCytochromeOxidaseenzymaticactivityin96-wellplateformat.Mitochondrialrespirationstudies,assemblyofthecomplexes,Mitochondriaoutermembraneintegrity.III.SampleType:PurifiedmitochondriaCells/TissueextractsIV.KitContents:V.UserSuppliedReagentsandEquipment:Multi-wellspectrophotometercapableofreadingabsorbance.Multi-channelpipetVI.StorageandHand领:Storekitat-20°C,protectedfromlight.WarmAssayBuffertoroomtemperaturebeforeuse.KeepEnzymeDilutionBufferonice.Readtheentireprotocolbeforeperformingtheassay.VII.ReagentPreparationandStorageConditions:DTT:Aliquotandstoreat-20°C.Thawjustbeforeuse.Cytochromec:Reconstituteeachvialwith1mlofCytochromeOxidaseAssayBuffer.Mixbyvortexingtodissolvecompletely.Add5<lofDTTsolution.Mixwellandwaitfor15min.atroomtemperature.Keepthisworkingsolutionatroomtemperature.Afterassayiscompleted,aliquotandsaverestoftheCytochromecsolutionat-20°C.ThisisnowreducedformofCytochromec.VIII.ComplexIVActivityAssayProtocol:1.EfficiencyofReductionofCytochromec:Ina96-wellplate,mix20<lofreducedCytochromecwith100<lofCytochromeOxidaseAssayBuffer.PrepareaparallelwellasblankwithonlyAssayBuffer.ReadODat550nm.TheODat550nmofreducedCytochromecisbetween0.2-0.6.Ifnot,add5<lofDTT/mlofreconstitutedCytochromecandwaitfor15min.toreadagaintheOD.2.SamplePreparation:Isolatemitochondriafromculturedcells,yeastortissuesbyusingMitochondria/CytosolFractionationKit(BVcat.#K256)orYeastMitochondriaIsolationKit(K259-50)orusecellortissuelysate(BVcat.#1067).Therecommendedrangeofpurifiedmitochondriais0.5-5<gandtissueextractis1-60<gperreaction.Dilutethetestsamples,ifneededbyEnzymeDilutionBuffer.3.Cytochromecpreparation:Prepare1:6dilutionofCytochromecbyusingpre-warmedCytochromeOxidaseAssayBuffer(onepartofCytochromecto5partsofbuffer)inaseparatetubedependingonthenumberofassaysamplesandcontrols.Prepare120<lofdilutedCytochromecperreaction.4.ComplexIVActivityAssay:Beforethereaction,setthespectrophotometerat550nmonkineticprogramfor30-45minutesat30secinterval.Addthetestsamples(approx.volume5-10<l)toeachwellofa96-wellplate.Fornegativecontrol(Blank),addequalvolumeofEnzymeDilutionBuffer.Add120<lofthedilutedCytochromecfromStep3toeachsampleandcontrolusingamultichannelpipette.ShakeandimmediatelyreadandrecorddecreaseinODoveraperiodof30-45min.Note:Therateofthereactionisrelativetoacontrolornormalsample.Therateiscalculatedinlinearrange.5.Calculations:CalculaterateofthereactionbycalculatingchangeinOD:OD/minbyusingthemaximumlinearrate.Theoxidation[详细]
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2018-11-08 10:00
产品样册
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报价单
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- Amphiphilic Molecular Design as a Rational Strategy
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2008-11-14 00:00
应用文章
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2008-02-15 00:00
报价单
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2024-09-20 13:34
报价单
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- Glass sample holders - improved design
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2024-09-29 11:09
操作手册
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- Respiration Activity MOnitoring System
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2008-05-05 00:00
产品样册
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- PR-water activity and bakery products
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2024-09-28 01:07
其它
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- Microwave Synthesis for Everyone
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2006-01-05 00:00
报价单
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- Microwave-Assisted Polymer Synthesis
- Microwave-Assisted Polymer Synthesis[详细]
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2006-04-30 00:00
应用文章
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- Microwave-assisted synthesis of allylic
- hehydrozirconationofalkyneswithzirconocenehydrochlorideandthedimethylzinc-mediatedadditionofalkenylzirconocenestodiphenylphosphinoyliminescanbegreatlyacceleratedwithmicrowaveirradiation,resultinginaconvenientandrapidone-potprocessforthepreparationofsyntheticallyusefulallylicamines.[详细]
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2018-08-17 10:00
产品样册
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- Microwave-Assisted Stepwise synthesis
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2024-09-29 14:37
实验操作
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- Foods vs. aw (water activity )
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2024-09-27 23:46
选购指南
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- Respiratory burst activity of Atlantic c
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2024-09-14 19:12
标准
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- Structure and biological activity of pathogen-like s
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2024-09-20 13:30
产品样册
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- Electrochemical synthesis and characterization of Ti
- Electrochemical synthesis and characterization of Ti[详细]
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2008-09-24 00:00
实验操作
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- Synthesis and piezoelectric properties of nanocrysta
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2007-08-29 00:00
报价单
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- Synthesis and electro-polymerisation of a novel hete
- Synthesis and electro-polymerisation of a novel hete[详细]
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2015-02-27 00:00
操作手册
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