Effect of Glutaraldehyde on the Conformational Propertiesof Kluyveromyceslactis β-Galactosidase and

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2018-08-16 10:00 1046阅读次数

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  TheeffectofglutaraldehydeontheconformationalpropertiesofKluyveromyceslactisβ-galactosidasewasdetermined.Theoptimumsubstrateoftheenzymeinterchangedfromlactosetosucrosewhenitreactedwithacertainconcentrationofglutaraldehyde.Itsmechanismwasstudiedbydetectingthechangesinfluorescenceabsorption.Thisisthefirstdiscoverythatβ-galactosidasecanbeconvertedtoanotherkindofenzymewhichcancatalyzesucrosetoproduceglucoseratherthanlactose.Onthebasisofthisphenomenon,anamperometricsucrosebiosensorusingβ-galactosidasemodifiedbyglutaraldehydeastheimmobilizedenzymeandpoly-β-cyclodextrinasthecarrierwasprepared.Theresponsecharacteristicofthesucrosebiosensorwasalsostudied.Underoptimumconditions,thecurrentresponsehadalinearrelationshipwiththesucroseconcentrationintherangeof02g/dLwithacorrelationcoefficientof0.9996andaresponsetimeof<30s.Thesucrosebiosensorexhibitedexcellentdiffusionandstability,andretaineditssensitivityafter7dofcontinuoususe.Thissucrosebiosensorisexpectedtoprovideaneffectiveandeconomicalalternativeforthequickandsensitivedetectionofsucroseinthefoodandbeverageindustriesinthefuture.[详细]

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